(Circular) (Six-base) MAPSORT of: pFN21AB8960.seq Check: 8115 from: 1 to: 5725 pFN21AB8960 5725 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2238 3068 1937 Size: 301 830 4594 Fragments arranged by size: 4594 830 301 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2126 1184 Size: 942 4783 AlwNI CAG_nnn'CTG Cuts at: 2067 2404 3468 5040 2067 Size: 337 1064 1572 2752 Fragments arranged by size: 2752 1572 1064 337 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2660 3050 1870 Size: 73 717 390 4545 Fragments arranged by size: 4545 717 390 73 BamHI G'GATC_C Cuts at: 2991 3055 3422 2991 Size: 64 367 5294 Fragments arranged by size: 5294 367 64 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2814 3046 4501 618 Size: 325 217 627 1027 232 1455 1842 Fragments arranged by size: 1842 1455 1027 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2616 2634 2660 3044 1391 Size: 1225 18 26 384 4072 Fragments arranged by size: 4072 1225 384 26 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BbvCI CC'TCA_GC Cuts at: 2703 2703 Size: 5725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 2058 1548 Size: 510 5215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2517 4336 136 Size: 107 122 71 631 605 845 1819 1525 Fragments arranged by size: 1819 1525 845 631 605 122 107 71 BglII A'GATC_T Cuts at: 2261 5720 2261 Size: 3459 2266 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2819 3786 4942 1790 Size: 1029 967 1156 2573 Fragments arranged by size: 2573 1156 1029 967 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4414 442 Size: 416 53 83 399 24 2039 958 1753 Fragments arranged by size: 2039 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 4385 1239 Size: 1283 1863 2579 Fragments arranged by size: 2579 1863 1283 Bpu10I CC'TnA_GC Cuts at: 2703 2703 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2217 2951 3850 4715 5013 2217 Size: 734 899 865 298 2929 Fragments arranged by size: 2929 899 865 734 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2713 4388 882 Size: 617 1214 1675 2219 Fragments arranged by size: 2219 1675 1214 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2988 4158 4556 4830 4977 5388 2988 Size: 1170 398 274 147 411 3325 Fragments arranged by size: 3325 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 1178 Size: 30 165 30 662 30 4808 Fragments arranged by size: 4808 662 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2704 1186 Size: 1518 4207 BseYI C'CCAG_C Cuts at: 2583 4928 5424 2583 Size: 2345 496 2884 Fragments arranged by size: 2884 2345 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 1163 Size: 573 1014 4138 Fragments arranged by size: 4138 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2382 3175 3936 4964 1540 Size: 275 186 381 793 761 1028 2301 Fragments arranged by size: 2301 1028 793 761 381 275 186 BsiHKAI G_wGCw'C Cuts at: 3786 3871 4942 3786 Size: 85 1071 4569 Fragments arranged by size: 4569 1071 85 BsmI GAATG_Cn' Cuts at: 2151 3236 3329 2151 Size: 1085 93 4547 Fragments arranged by size: 4547 1085 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2175 2175 Size: 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2616 2634 2660 2819 3044 3786 3871 Size: 399 826 18 26 159 225 742 85 Cuts at: 3871 4942 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 826 742 399 225 159 85 26 18 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2920 3080 844 Size: 2076 160 3489 Fragments arranged by size: 3489 2076 160 BsrBI CCG'CTC Cuts at: 1394 1823 2622 3612 1394 Size: 429 799 990 3507 Fragments arranged by size: 3507 990 799 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 2106 96 Size: 2010 3715 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2550 3785 4797 2550 Size: 1235 1012 3478 Fragments arranged by size: 3478 1235 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2251 2261 2991 3055 3422 3807 3824 5276 Size: 279 10 730 64 367 385 17 1452 Cuts at: 5276 5362 5374 5720 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 730 385 367 346 279 86 64 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2436 2436 Size: 5725 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3241 4035 4055 2787 Size: 454 794 20 4457 Fragments arranged by size: 4457 794 454 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2533 2568 529 Size: 35 1969 35 3686 Fragments arranged by size: 3686 1969 35 35 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2930 4732 809 Size: 507 1614 1802 1802 Fragments arranged by size: 1802 1802 1614 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2379 2559 Size: 54 1425 50 275 18 345 204 180 Cuts at: 2559 2623 3172 4061 5690 8 Size: 64 549 889 1629 43 Fragments arranged by size: 1629 1425 889 549 345 275 204 180 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2379 3172 1537 Size: 275 567 793 4090 Fragments arranged by size: 4090 793 567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 2804 3655 1353 Size: 1326 125 851 3423 Fragments arranged by size: 3423 1326 851 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3788 4385 5172 5440 1239 Size: 393 890 1266 597 787 268 1524 Fragments arranged by size: 1524 1266 890 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2702 2702 Size: 5725 EcoO109I rG'GnC_Cy Cuts at: 2136 2386 2136 Size: 250 5475 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2693 2693 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2100 2890 4872 1678 Size: 422 790 1982 2531 Fragments arranged by size: 2531 1982 790 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2914 3069 3315 669 Size: 988 281 976 155 246 3079 Fragments arranged by size: 3079 988 976 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 2914 3315 2914 Size: 401 5324 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 2338 2779 3324 2338 Size: 441 545 4739 Fragments arranged by size: 4739 545 441 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2225 4839 5023 1305 Size: 920 2614 184 2007 Fragments arranged by size: 2614 2007 920 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 3558 Fragments arranged by size: 3558 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2531 3683 4042 4201 518 Size: 648 942 423 1152 359 159 2042 Fragments arranged by size: 2042 1152 942 648 423 359 159 MspA1I CmG'CkG Cuts at: 1606 2579 2587 3141 3818 4966 5211 1606 Size: 973 8 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 973 677 554 245 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2107 2113 2350 3083 4628 1132 Size: 97 878 6 237 733 1545 2229 Fragments arranged by size: 2229 1545 878 733 237 97 6 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 2404 2781 2839 1108 Size: 1095 201 377 58 3994 Fragments arranged by size: 3994 1095 377 201 58 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2846 2878 3794 3826 5307 5339 2846 Size: 32 916 32 1481 32 3232 Fragments arranged by size: 3232 1481 916 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2386 2136 Size: 250 5475 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 2072 2592 3077 830 Size: 1242 520 485 3478 Fragments arranged by size: 3478 1242 520 485 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2587 2587 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 2072 3077 2072 Size: 1005 4720 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2068 2588 3073 4208 4889 5080 826 Size: 219 1023 520 485 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 681 520 485 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2232 2966 3829 4730 4992 820 Size: 197 926 289 734 863 901 262 1553 Fragments arranged by size: 1553 926 901 863 734 289 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 3164 513 Size: 552 2099 3074 Fragments arranged by size: 3074 2099 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2499 3971 Size: 274 80 33 51 1443 129 393 1472 Cuts at: 3971 5683 96 Size: 1712 138 Fragments arranged by size: 1712 1472 1443 393 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 5685 5717 2280 Size: 32 3373 32 2288 Fragments arranged by size: 3373 2288 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2466 1316 Size: 1150 4575 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2778 2778 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI