(Circular) (Six-base) MAPSORT of: pFN21AB8955.seq Check: 6913 from: 1 to: 5701 pFN21AB8955 5701 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 3044 1937 Size: 370 737 4594 Fragments arranged by size: 4594 737 370 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2269 2627 3764 5148 5416 1632 Size: 637 358 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1137 637 358 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 3010 3236 3388 5497 1493 Size: 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 2364 2668 3026 1870 Size: 73 421 304 358 4545 Fragments arranged by size: 4545 421 358 304 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3022 4477 618 Size: 325 217 627 1235 1455 1842 Fragments arranged by size: 1842 1455 1235 627 325 217 BanII G_rGCy'C Cuts at: 1391 2178 2279 3020 1391 Size: 787 101 741 4072 Fragments arranged by size: 4072 787 741 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2098 2132 3892 3926 1903 Size: 34 50 34 77 34 1760 34 3678 Fragments arranged by size: 3678 1760 77 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 4312 136 Size: 107 122 71 631 605 493 2147 1525 Fragments arranged by size: 2147 1525 631 605 493 122 107 71 BglII A'GATC_T Cuts at: 2616 5696 2616 Size: 3080 2621 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2105 3762 4918 1790 Size: 315 1657 1156 2573 Fragments arranged by size: 2573 1657 1156 315 BmgBI CAC'GTC Cuts at: 1318 2164 1318 Size: 846 4855 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 4390 442 Size: 416 53 83 399 24 2015 958 1753 Fragments arranged by size: 2015 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2063 2666 2905 4361 1239 Size: 794 30 603 239 1456 2579 Fragments arranged by size: 2579 1456 794 603 239 30 Bpu10I CC'TnA_GC Cuts at: 2336 2790 2336 Size: 454 5247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 3492 493 Size: 711 831 1457 2702 Fragments arranged by size: 2702 1457 831 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 3508 3890 4525 Size: 53 83 186 936 590 1385 382 635 Cuts at: 4525 275 Size: 1451 Fragments arranged by size: 1451 1385 936 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 4134 4532 4806 4953 5364 2880 Size: 1254 398 274 147 411 3217 Fragments arranged by size: 3217 1254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5701 BseYI C'CCAG_C Cuts at: 2969 4904 5400 2969 Size: 1935 496 3270 Fragments arranged by size: 3270 1935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 2449 1163 Size: 573 467 246 4415 Fragments arranged by size: 4415 573 467 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2160 3151 3912 4940 1540 Size: 275 186 159 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 275 186 159 BsiHKAI G_wGCw'C Cuts at: 2268 2279 3762 3847 4918 2268 Size: 11 1483 85 1071 3051 Fragments arranged by size: 3051 1483 1071 85 11 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2178 2268 2279 3020 3762 3847 Size: 399 315 73 90 11 741 742 85 Cuts at: 3847 4918 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 741 399 315 90 85 73 11 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 844 Size: 2212 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2335 3588 1394 Size: 429 512 1253 3507 Fragments arranged by size: 3507 1253 512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2379 2823 4196 4370 65 Size: 1183 1131 444 1373 174 1396 Fragments arranged by size: 1396 1373 1183 1131 444 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 2745 1184 Size: 1561 4140 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2209 2710 2722 2011 Size: 198 501 12 4990 Fragments arranged by size: 4990 501 198 12 BstYI r'GATC_y Cuts at: 1972 2616 2691 3031 3398 3783 3800 5252 5338 Size: 644 75 340 367 385 17 1452 86 Cuts at: 5338 5350 5696 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 644 385 367 346 340 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2169 2298 513 Size: 552 1029 75 129 3916 Fragments arranged by size: 3916 1029 552 129 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 487 Size: 683 56 812 4150 Fragments arranged by size: 4150 812 683 56 BtsI GCAGTG_nn' Cuts at: 2585 3217 4011 4031 2585 Size: 632 794 20 4255 Fragments arranged by size: 4255 794 632 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2166 2295 Size: 54 1425 50 275 18 327 9 129 Cuts at: 2295 2319 2712 2826 3148 4037 5666 8 Size: 24 393 114 322 889 1629 43 Fragments arranged by size: 1629 1425 889 393 327 322 275 129 114 54 50 43 24 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2157 3148 1537 Size: 275 345 991 4090 Fragments arranged by size: 4090 991 345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 2246 3631 1353 Size: 830 63 1385 3423 Fragments arranged by size: 3423 1385 830 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2277 2277 Size: 5701 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2063 2269 2627 2666 2905 3764 Size: 393 401 30 206 358 39 239 859 Cuts at: 3764 4361 5148 5416 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 859 787 597 401 393 358 268 239 206 39 30 EcoO109I rG'GnC_Cy Cuts at: 2361 2395 2361 Size: 34 5667 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 5701 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 2042 4848 1678 Size: 364 2806 2531 Fragments arranged by size: 2806 2531 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4342 4374 4416 4448 1373 Size: 32 135 32 2770 32 42 32 2626 Fragments arranged by size: 2770 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 2764 3045 3291 669 Size: 988 281 370 456 281 246 3079 Fragments arranged by size: 3079 988 456 370 281 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 2764 3291 2764 Size: 527 5174 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2714 2828 10 Size: 54 1425 343 489 393 114 2883 Fragments arranged by size: 2883 1425 489 393 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3659 4018 4177 518 Size: 648 1554 939 359 159 2042 Fragments arranged by size: 2042 1554 939 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2686 3117 3794 4942 5187 1606 Size: 490 590 431 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 490 431 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 2759 2846 3059 4604 1132 Size: 97 1530 87 213 1545 2229 Fragments arranged by size: 2229 1545 1530 213 97 87 PasI CC'CwG_GG Cuts at: 1348 2475 1348 Size: 1127 4574 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2361 2395 2361 Size: 34 5667 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 2594 3271 2594 Size: 677 5024 PspXI vC'TCGA_Gb Cuts at: 2668 2668 Size: 5701 PstI C_TGCA'G Cuts at: 830 2121 2636 3053 830 Size: 1291 515 417 3478 Fragments arranged by size: 3478 1291 515 417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SacI G_AGCT'C Cuts at: 2279 2279 Size: 5701 SacII CC_GC'GG Cuts at: 2097 2097 Size: 5701 SalI G'TCGA_C Cuts at: 1936 2306 3043 1936 Size: 370 737 4594 Fragments arranged by size: 4594 737 370 SanDI GG'GwC_CC Cuts at: 2361 2361 Size: 5701 SapI GCTCTTCn'nnn_ Cuts at: 2183 2183 Size: 5701 SbfI CC_TGCA'GG Cuts at: 2636 3053 2636 Size: 417 5284 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2117 2632 3049 4184 4865 5056 826 Size: 219 1072 515 417 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 515 417 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2165 2165 Size: 5701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2668 3805 4706 4968 820 Size: 197 926 725 1137 901 262 1553 Fragments arranged by size: 1553 1137 926 901 725 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 2759 3059 1132 Size: 1627 300 3774 Fragments arranged by size: 3774 1627 300 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5701 StyI C'CwwG_G Cuts at: 513 1065 3140 513 Size: 552 2075 3074 Fragments arranged by size: 3074 2075 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2559 3728 3745 3898 4083 4528 325 Size: 1255 979 1169 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1169 979 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2137 2430 2545 2137 Size: 293 115 5293 Fragments arranged by size: 5293 293 115 XhoI C'TCGA_G Cuts at: 1943 2668 1943 Size: 725 4976 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SexAI SfoI SrfI SwaI