Restriction map for
pFN21AB8935
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeII
MamI MluI MroI MslI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 561 AAATTT [GA]^AATT[CT]
AflIII 733 ACATGT A^C[GA][CT]GT
AlwI 454 GGATC GGATC(4/5)
AocI 250 CCTGAGG CC^T[ACGT]AGG
ApoI 561 AAATTT [GA]^AATT[CT]
BalI 698 TGGCCA TGG^CCA
BanI 345 GGCACC G^G[CT][GA]CC
BcgI 229 GCAATTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 344 GGGCAC G[AGT]GC[ACT]^C
BsgI 367 GTGCAG GTGCAG(16/14)
BsiYI 335 CCTTTCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 335 CCTTTCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 712 GGGAC GGGAC(10/14)
Bsp1286I 344 GGGCAC G[AGT]GC[ACT]^C
BspDI 836 ATCGAT AT^CGAT
BspLU11I 733 ACATGT A^CATGT
BspMI 225 ACCTGC ACCTGC(4/8)
BstBI 404 TTCGAA TT^CGAA
BstXI 26 CCAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 454 GGATCT [GA]^GATC[CT]
Bsu36I 250 CCTGAGG CC^T[ACGT]AGG
ClaI 836 ATCGAT AT^CGAT
DpnII 455 GATC ^GATC
DraII 712 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 7 GACGAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 698 TGGCCA [CT]^GGCC[GA]
EarI 401 CTCTTC CTCTTC(1/4)
Eco81I 250 CCTGAGG CC^T[ACGT]AGG
EcoO109I 712 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 531 GATATC GAT^ATC
Ksp632I 401 CTCTTC CTCTTC(1/4)
MboI 455 GATC ^GATC
MfeI 428 CAATTG C^AATTG
MflI 454 GGATCT [GA]^GATC[CT]
MluNI 698 TGGCCA TGG^CCA
MscI 698 TGGCCA TGG^CCA
MspA1I 276 CAGCGG C[AC]G^C[GT]G
MunI 428 CAATTG C^AATTG
NdeII 455 GATC ^GATC
NspI 733 ACATGT [GA]CATG^[CT]
NspV 404 TTCGAA TT^CGAA
PaeR7I 314 CTCGAG C^TCGAG
PpuMI 712 GGGACCC [GA]G^G[AT]CC[CT]
SapI 400 GCTCTTC GCTCTTC(1/4)
Sau3AI 455 GATC ^GATC
ScaI 268 AGTACT AGT^ACT
SfuI 404 TTCGAA TT^CGAA
SmlI 314 CTCGAG C^T[CT][GA]AG
Tsp45I 802 GTGAC ^GT[GC]AC
TspRI 800 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 314 CTCGAG C^TCGAG
XhoII 454 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 18 CCGC CCGC(-3/-1)
278 GCGG
AvaI 314 CTCGAG C^[CT]CG[GA]G
341 CTCGGG
AvaII 15 GGTCC G^G[AT]CC
713 GGACC
BbvI 397 GCAGC GCAGC(8/12)
394 GCTGC
BfaI 611 CTAG C^TAG
635 CTAG
BsaJI 59 CCCTGG C^C[ACGT][ACGT]GG
340 CCTCGG
BsmAI 304 GAGAC GTCTC(1/5)
150 GTCTC
BsoBI 314 CTCGAG C^[CT]CG[GA]G
341 CTCGGG
BsrDI 423 CATTGC GCAATG(2/0)
281 GCAATG
BsrI 706 ACTGG ACTGG(1/-1)
799 CCAGT
BssKI 60 CCTGG ^CC[ACGT]GG
164 CCTGG
BstNI 60 CCTGG CC^[AT]GG
164 CCTGG
Cac8I 110 GCAAGC GC[ACGT]^[ACGT]GC
390 GCTTGC
DdeI 194 CTGAG C^T[ACGT]AG
251 CTGAG
EcoRII 60 CCTGG ^CC[AT]GG
164 CCTGG
HaeIII 185 GGCC GG^CC
699 GGCC
HinfI 83 GAATC G^A[ACGT]TC
361 GATTC
MaeI 611 CTAG C^TAG
635 CTAG
MaeIII 133 GTTAC ^GT[ACGT]AC
802 GTGAC
MvaI 60 CCTGG CC^[AT]GG
164 CCTGG
MwoI 281 GCAATGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
427 GCAATTGGAGC
NlaIII 501 CATG CATG^
734 CATG
PstI 70 CTGCAG CTGCA^G
395 CTGCAG
ScrFI 60 CCTGG CC^[ACGT]GG
164 CCTGG
SfcI 70 CTGCAG C^T[GA][CT]AG
395 CTGCAG
SspI 46 AATATT AAT^ATT
589 AATATT
TfiI 83 GAATC G^A[AT]TC
361 GATTC
TseI 394 GCTGC G^C[AT]GC
397 GCAGC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI BstF5I Cfr13I Fnu4HI
FokI ItaI MboII MnlI MseI
NlaIV RsaI Sau96I SfaNI TaqI
Tru9I Tsp509I TthHB8I