Restriction map for
pFN21AB8925
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MaeI MaeII
MamI MfeI MluI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 452 GGTACC G^GTACC
AccIII 93 TCCGGA T^CCGGA
AocI 286 CCTCAGG CC^T[ACGT]AGG
ApaI 112 GGGCCC GGGCC^C
Asp700I 186 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 452 GGTACC G^GTACC
BglII 518 AGATCT A^GATCT
BpmI 427 CTGGAG CTGGAG(16/14)
BsaI 349 GAGACC GGTCTC(1/5)
BseAI 93 TCCGGA T^CCGGA
BseRI 70 GAGGAG GAGGAG(10/8)
BsiEI 320 CGGCCG CG[GA][CT]^CG
BsmAI 349 GAGAC GTCTC(1/5)
BsmI 692 GCATTC GAATGC(1/-1)
BspEI 93 TCCGGA T^CCGGA
BsrBI 430 GAGCGG CCGCTC(-3/-3)
BsrFI 373 GCCGGC [GA]^CCGG[CT]
BstF5I 492 CATCC GGATG(2/0)
BstYI 518 AGATCT [GA]^GATC[CT]
Bsu36I 286 CCTCAGG CC^T[ACGT]AGG
Cfr10I 373 GCCGGC [GA]^CCGG[CT]
DpnII 519 GATC ^GATC
EagI 320 CGGCCG C^GGCCG
EarI 100 GAAGAG CTCTTC(1/4)
EclXI 320 CGGCCG C^GGCCG
Eco52I 320 CGGCCG C^GGCCG
Eco81I 286 CCTCAGG CC^T[ACGT]AGG
EcoNI 792 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 407 CCAAGG C^C[AT][AT]GG
FokI 492 CATCC GGATG(9/13)
KpnI 452 GGTACC GGTAC^C
Ksp632I 100 GAAGAG CTCTTC(1/4)
MboI 519 GATC ^GATC
MflI 518 AGATCT [GA]^GATC[CT]
MroI 93 TCCGGA T^CCGGA
NaeI 373 GCCGGC GCC^GGC
NdeII 519 GATC ^GATC
NgoMIV 373 GCCGGC G^CCGGC
NspI 731 ACATGC [GA]CATG^[CT]
PflMI 660 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 662 AGGACCT [GA]G^G[AT]CC[CT]
PstI 724 CTGCAG CTGCA^G
Sau3AI 519 GATC ^GATC
ScaI 251 AGTACT AGT^ACT
SmlI 294 CTCAAG C^T[CT][GA]AG
StyI 407 CCAAGG C^C[AT][AT]GG
TspRI 808 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 660 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 518 AGATCT [GA]^GATC[CT]
XmnI 186 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BalI 230 TGGCCA TGG^CCA
482 TGGCCA
BglI 124 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
138 GCCGCTGGGGC
BsaWI 93 TCCGGA [AT]^CCGG[AT]
333 ACCGGA
BsgI 259 CTGCAC GTGCAG(16/14)
166 GTGCAG
BspMI 50 ACCTGC ACCTGC(4/8)
510 GCAGGT
BsrI 747 CCAGT ACTGG(1/-1)
771 CCAGT
BssHII 552 GCGCGC G^CGCGC
713 GCGCGC
DdeI 45 CTTAG C^T[ACGT]AG
287 CTCAG
FauI 362 GCGGG CCCGC(4/6)
688 GCGGG
HinfI 292 GACTC G^A[ACGT]TC
328 GAGTC
HphI 579 GGTGA GGTGA(8/7)
331 TCACC
MboII 100 GAAGA GAAGA(8/7)
183 GAAGA
MluNI 230 TGGCCA TGG^CCA
482 TGGCCA
MscI 230 TGGCCA TGG^CCA
482 TGGCCA
MspA1I 139 CCGCTG C[AC]G^C[GT]G
448 CAGCGG
NlaIII 641 CATG CATG^
732 CATG
PleI 292 GACTC GAGTC(4/5)
328 GAGTC
SfcI 708 CTACAG C^T[GA][CT]AG
724 CTGCAG
SmaI 354 CCCGGG CCC^GGG
557 CCCGGG
XmaCI 354 CCCGGG C^CCGGG
557 CCCGGG
XmaI 354 CCCGGG C^CCGGG
557 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstAPI BstNI BstUI
Cac8I CfoI Cfr13I DraII EaeI
EcoO109I EcoRII Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MaeIII MnlI
MspI MvaI MvnI MwoI NarI
NciI NlaIV RsaI Sau96I ScrFI
SfoI TseI Tsp45I