(Circular) (Six-base) MAPSORT of: pFN21AB8925.seq Check: 9610 from: 1 to: 5524 pFN21AB8925 5524 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2457 2845 2457 Size: 388 5136 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 2237 3587 4971 5239 1632 Size: 575 30 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 575 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 2179 2671 2728 3267 4839 2179 Size: 492 57 539 1572 2864 Fragments arranged by size: 2864 1572 539 492 57 ApaI G_GGCC'C Cuts at: 2121 2121 Size: 5524 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2359 2471 2562 2849 1870 Size: 73 177 239 112 91 287 4545 Fragments arranged by size: 4545 287 239 177 112 91 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2284 2457 2467 2845 Size: 325 217 627 369 128 173 10 378 Cuts at: 2845 4300 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 378 369 325 217 173 128 10 BanII G_rGCy'C Cuts at: 1391 2084 2121 2322 2843 1391 Size: 693 37 201 521 4072 Fragments arranged by size: 4072 693 521 201 37 BbeI G_GCGC'C Cuts at: 2160 2288 2471 2160 Size: 128 183 5213 Fragments arranged by size: 5213 183 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 2149 4135 Size: 107 122 71 631 605 463 14 1986 Cuts at: 4135 136 Size: 1525 Fragments arranged by size: 1986 1525 631 605 463 122 107 71 14 BglII A'GATC_T Cuts at: 2523 5519 2523 Size: 2996 2528 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 2121 2622 3585 4741 1790 Size: 331 501 963 1156 2573 Fragments arranged by size: 2573 1156 963 501 331 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2746 2770 3255 Size: 416 53 83 399 24 1329 24 485 Cuts at: 3255 4213 442 Size: 958 1753 Fragments arranged by size: 1753 1329 958 485 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 4184 1239 Size: 1214 1731 2579 Fragments arranged by size: 2579 1731 1214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2284 3649 4514 4812 2284 Size: 1365 865 298 2996 Fragments arranged by size: 2996 1365 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2348 4187 882 Size: 617 849 1839 2219 Fragments arranged by size: 2219 1839 849 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2285 2376 2468 Size: 53 83 186 936 624 128 91 92 Cuts at: 2468 2588 3331 3713 4348 275 Size: 120 743 382 635 1451 Fragments arranged by size: 1451 936 743 635 624 382 186 128 120 92 91 83 53 BsaWI w'CCGG_w Cuts at: 2098 2338 3957 4355 4629 4776 5187 2098 Size: 240 1619 398 274 147 411 2435 Fragments arranged by size: 2435 1619 411 398 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2288 2318 1178 Size: 30 165 30 885 30 4384 Fragments arranged by size: 4384 885 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 1186 Size: 904 4620 BseYI C'CCAG_C Cuts at: 2146 2808 4727 5223 2146 Size: 662 1919 496 2447 Fragments arranged by size: 2447 1919 662 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2249 1163 Size: 573 456 57 4438 Fragments arranged by size: 4438 573 456 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2328 2974 3735 4763 1540 Size: 275 186 327 646 761 1028 2301 Fragments arranged by size: 2301 1028 761 646 327 275 186 BsiHKAI G_wGCw'C Cuts at: 3585 3670 4741 3585 Size: 85 1071 4368 Fragments arranged by size: 4368 1071 85 BsmI GAATG_Cn' Cuts at: 2697 3035 3128 2697 Size: 338 93 5093 Fragments arranged by size: 5093 338 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2121 2322 2622 2843 3585 3670 Size: 399 294 37 201 300 221 742 85 Cuts at: 3670 4741 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 294 221 201 85 37 BspEI T'CCGG_A Cuts at: 2098 5187 2098 Size: 3089 2435 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 2506 2879 844 Size: 1220 442 373 3489 Fragments arranged by size: 3489 1220 442 373 BsrBI CCG'CTC Cuts at: 1394 1823 2437 3411 1394 Size: 429 614 974 3507 Fragments arranged by size: 3507 974 614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2378 4168 4355 1700 Size: 110 568 1790 187 2869 Fragments arranged by size: 2869 1790 568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 2557 2718 1925 Size: 632 161 4731 Fragments arranged by size: 4731 632 161 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 2179 2728 2737 794 Size: 1385 549 9 3581 Fragments arranged by size: 3581 1385 549 9 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2523 2854 3221 3606 3623 5075 5161 5173 Size: 551 331 367 385 17 1452 86 12 Cuts at: 5173 5519 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 551 385 367 346 331 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2292 2292 Size: 5524 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4972 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 2819 3040 3834 3854 2819 Size: 221 794 20 4489 Fragments arranged by size: 4489 794 221 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2235 2325 Size: 54 1425 50 275 18 258 147 90 Cuts at: 2325 2487 2613 2763 2971 3860 5489 8 Size: 162 126 150 208 889 1629 43 Fragments arranged by size: 1629 1425 889 275 258 208 162 150 147 126 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2325 2971 1537 Size: 275 513 646 4090 Fragments arranged by size: 4090 646 513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 3454 1353 Size: 747 1354 3423 Fragments arranged by size: 3423 1354 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 4111 4485 4631 387 Size: 1064 138 900 1622 374 146 1280 Fragments arranged by size: 1622 1280 1064 900 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2237 2453 3587 4184 4971 5239 Size: 393 575 30 216 1134 597 787 268 Cuts at: 5239 1239 Size: 1524 Fragments arranged by size: 1524 1134 787 597 575 393 268 216 30 EcoNI CCTnn'n_nnAGG Cuts at: 2801 2801 Size: 5524 EcoO109I rG'GnC_Cy Cuts at: 2117 2248 2668 2117 Size: 131 420 4973 Fragments arranged by size: 4973 420 131 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2065 2212 2065 Size: 147 5377 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2160 2288 2443 2471 4671 1678 Size: 482 128 155 28 2200 2531 Fragments arranged by size: 2531 2200 482 155 128 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 4165 4197 4239 Size: 32 135 32 716 32 1845 32 42 Cuts at: 4239 4271 1373 Size: 32 2626 Fragments arranged by size: 2626 1845 716 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2156 2284 2467 2156 Size: 128 183 5213 Fragments arranged by size: 5213 183 128 KpnI G_GTAC'C Cuts at: 2461 2849 2461 Size: 388 5136 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 2489 10 Size: 54 1425 343 405 252 3045 Fragments arranged by size: 3045 1425 405 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 3482 3841 4000 518 Size: 648 2316 359 159 2042 Fragments arranged by size: 2316 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2455 2940 3617 4765 5010 1606 Size: 540 309 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 485 309 245 NaeI GCC'GGC Cuts at: 2380 2380 Size: 5524 NarI GG'CG_CC Cuts at: 2157 2285 2468 2157 Size: 128 183 5213 Fragments arranged by size: 5213 183 128 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4972 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NgoMIV G'CCGG_C Cuts at: 2378 2378 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2740 2882 4427 1132 Size: 97 1511 142 1545 2229 Fragments arranged by size: 2229 1545 1511 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 1108 Size: 1563 3961 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2668 2668 Size: 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PspOMI G'GGCC_C Cuts at: 2117 2117 Size: 5524 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2183 2215 2528 2560 2183 Size: 32 313 32 5147 Fragments arranged by size: 5147 313 32 32 PstI C_TGCA'G Cuts at: 830 2733 2876 830 Size: 1903 143 3478 Fragments arranged by size: 3478 1903 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 2258 3772 5484 2258 Size: 1514 1712 2298 Fragments arranged by size: 2298 1712 1514 SfcI C'TryA_G Cuts at: 826 1045 2713 2729 2872 4007 4688 4879 826 Size: 219 1668 16 143 1135 681 191 1471 Fragments arranged by size: 1668 1471 1135 681 219 191 143 16 SfoI GGC'GCC Cuts at: 2158 2286 2469 2158 Size: 128 183 5213 Fragments arranged by size: 5213 183 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2361 2564 2851 1872 Size: 489 203 287 4545 Fragments arranged by size: 4545 489 287 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 2299 3628 4529 4791 820 Size: 197 926 356 1329 901 262 1553 Fragments arranged by size: 1553 1329 926 901 356 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SrfI GCCC'GGGC Cuts at: 2564 2564 Size: 5524 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2412 2963 513 Size: 552 1347 551 3074 Fragments arranged by size: 3074 1347 552 551 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 3551 3568 3721 3906 4351 325 Size: 1255 446 1525 17 153 185 445 1498 Fragments arranged by size: 1525 1498 1255 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2742 2754 Size: 274 80 33 51 1443 279 486 12 Cuts at: 2754 3770 5482 96 Size: 1016 1712 138 Fragments arranged by size: 1712 1443 1016 486 279 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2359 2562 2849 1870 Size: 489 203 287 4545 Fragments arranged by size: 4545 489 287 203 XmnI GAAnn'nnTTC Cuts at: 2195 3653 2195 Size: 1458 4066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI