Restriction map for pFN21AB8925
 (Linear) (Six-base) MAPSORT of: KIBB8925.seq  Check: 6989  from: 1  to: 819



KIBB8925.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

Acc65I G'GTAC_C

Cuts at:      0     452     819 
   Size:        452     367
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     232     819 
   Size:        202      30     587

  Fragments arranged by size: 

                 587     202      30
 
AlwNI CAG_nnn'CTG

Cuts at:      0     174     666     723     819 
   Size:        174     492      57      96

  Fragments arranged by size: 

                 492     174      96      57
 
ApaI G_GGCC'C

Cuts at:      0     116     819 
   Size:        116     703
 
AvaI C'yCGr_G

Cuts at:      0     115     354     466     557     819 
   Size:        115     239     112      91     262

  Fragments arranged by size: 

                 262     239     115     112      91
 
BanI G'GyrC_C

Cuts at:      0     151     279     452     462     819 
   Size:        151     128     173      10     357

  Fragments arranged by size: 

                 357     173     151     128      10
 
BanII G_rGCy'C

Cuts at:      0      79     116     317     819 
   Size:         79      37     201     502

  Fragments arranged by size: 

                 502     201      79      37
 
BbeI G_GCGC'C

Cuts at:      0     155     283     466     819 
   Size:        155     128     183     353

  Fragments arranged by size: 

                 353     183     155     128
 
BglI GCCn_nnn'nGGC

Cuts at:      0     130     144     819 
   Size:        130      14     675

  Fragments arranged by size: 

                 675     130      14
 
BglII A'GATC_T

Cuts at:      0     518     819 
   Size:        518     301
 
Bme1580I G_kGCm'C

Cuts at:      0     116     617     819 
   Size:        116     501     202

  Fragments arranged by size: 

                 501     202     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     741     765     819 
   Size:        741      24      54

  Fragments arranged by size: 

                 741      54      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      93     819 
   Size:         93     726
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     819 
   Size:        448     371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     819 
   Size:        279     540
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     343     819 
   Size:        343     476
 
BsaHI Gr'CG_yC

Cuts at:      0     152     280     371     463     583     819 
   Size:        152     128      91      92     120     236

  Fragments arranged by size: 

                 236     152     128     120      92      91
 
BsaWI w'CCGG_w

Cuts at:      0      93     333     819 
   Size:         93     240     486

  Fragments arranged by size: 

                 486     240      93
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     283     313     819 
   Size:        283      30     506

  Fragments arranged by size: 

                 506     283      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     819 
   Size:         85     734
 
BseYI C'CCAG_C

Cuts at:      0     141     803     819 
   Size:        141     662      16

  Fragments arranged by size: 

                 662     141      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     244     819 
   Size:        187      57     575

  Fragments arranged by size: 

                 575     187      57
 
BsiEI CG_ry'CG

Cuts at:      0     323     819 
   Size:        323     496
 
BsmI GAATG_Cn'

Cuts at:      0     692     819 
   Size:        692     127
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     116     317     617     819 
   Size:         79      37     201     300     202

  Fragments arranged by size: 

                 300     202     201      79      37
 
BspEI T'CCGG_A

Cuts at:      0      93     819 
   Size:         93     726
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      59     501     819 
   Size:         59     442     318

  Fragments arranged by size: 

                 442     318      59
 
BsrBI CCG'CTC

Cuts at:      0     432     819 
   Size:        432     387
 
BsrFI r'CCGG_y

Cuts at:      0     373     819 
   Size:        373     446
 
BssHII G'CGCG_C

Cuts at:      0     552     713     819 
   Size:        552     161     106

  Fragments arranged by size: 

                 552     161     106
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     174     723     732     819 
   Size:        174     549       9      87

  Fragments arranged by size: 

                 549     174      87       9
 
BstYI r'GATC_y

Cuts at:      0     518     819 
   Size:        518     301
 
Bsu36I CC'TnA_GG

Cuts at:      0     287     819 
   Size:        287     532
 
BtsI GCAGTG_nn'

Cuts at:      0     814     819 
   Size:        814       5
 
EaeI y'GGCC_r

Cuts at:      0      83     230     320     482     608     758     819 
   Size:         83     147      90     162     126     150      61

  Fragments arranged by size: 

                 162     150     147     126      90      83      61
 
EagI C'GGCC_G

Cuts at:      0     320     819 
   Size:        320     499
 
EarI CTCTTCn'nnn_

Cuts at:      0      95     819 
   Size:         95     724
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     484     819 
   Size:        484     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     232     448     819 
   Size:        202      30     216     371

  Fragments arranged by size: 

                 371     216     202      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     796     819 
   Size:        796      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     243     663     819 
   Size:        112     131     420     156

  Fragments arranged by size: 

                 420     156     131     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      60     207     819 
   Size:         60     147     612

  Fragments arranged by size: 

                 612     147      60
 
HaeII r_GCGC'y

Cuts at:      0     155     283     438     466     819 
   Size:        155     128     155      28     353

  Fragments arranged by size: 

                 353     155     155     128      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     819 
   Size:        283      32     504

  Fragments arranged by size: 

                 504     283      32
 
KasI G'GCGC_C

Cuts at:      0     151     279     462     819 
   Size:        151     128     183     357

  Fragments arranged by size: 

                 357     183     151     128
 
KpnI G_GTAC'C

Cuts at:      0     456     819 
   Size:        456     363
 
MscI TGG'CCA

Cuts at:      0     232     484     819 
   Size:        232     252     335

  Fragments arranged by size: 

                 335     252     232
 
MspA1I CmG'CkG

Cuts at:      0     141     450     819 
   Size:        141     309     369

  Fragments arranged by size: 

                 369     309     141
 
NaeI GCC'GGC

Cuts at:      0     375     819 
   Size:        375     444
 
NarI GG'CG_CC

Cuts at:      0     152     280     463     819 
   Size:        152     128     183     356

  Fragments arranged by size: 

                 356     183     152     128
 
NgoMIV G'CCGG_C

Cuts at:      0     373     819 
   Size:        373     446
 
NspI r_CATG'y

Cuts at:      0     735     819 
   Size:        735      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     666     819 
   Size:        666     153
 
PpuMI rG'GwC_Cy

Cuts at:      0     663     819 
   Size:        663     156
 
PspOMI G'GGCC_C

Cuts at:      0     112     819 
   Size:        112     707
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     178     210     523     555     819 
   Size:        178      32     313      32     264

  Fragments arranged by size: 

                 313     264     178      32      32
 
PstI C_TGCA'G

Cuts at:      0     728     819 
   Size:        728      91
 
ScaI AGT'ACT

Cuts at:      0     253     819 
   Size:        253     566
 
SfcI C'TryA_G

Cuts at:      0     708     724     819 
   Size:        708      16      95

  Fragments arranged by size: 

                 708      95      16
 
SfoI GGC'GCC

Cuts at:      0     153     281     464     819 
   Size:        153     128     183     355

  Fragments arranged by size: 

                 355     183     153     128
 
SmaI CCC'GGG

Cuts at:      0     356     559     819 
   Size:        356     203     260

  Fragments arranged by size: 

                 356     260     203
 
SmlI C'TyrA_G

Cuts at:      0     294     819 
   Size:        294     525
 
SrfI GCCC'GGGC

Cuts at:      0     559     819 
   Size:        559     260
 
StyI C'CwwG_G

Cuts at:      0     407     819 
   Size:        407     412
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     819 
   Size:         21     798
 
TatI w'GTAC_w

Cuts at:      0     251     737     749     819 
   Size:        251     486      12      70

  Fragments arranged by size: 

                 486     251      70      12
 
XmaI C'CCGG_G

Cuts at:      0     354     557     819 
   Size:        354     203     262

  Fragments arranged by size: 

                 354     262     203
 
XmnI GAAnn'nnTTC

Cuts at:      0     190     819 
   Size:        190     629
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BglI    BglII Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
   Bsu36I     BtsI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     KpnI     MscI   MspA1I
     NaeI     NarI   NgoMIV     NspI    PflMI    PpuMI   PspOMI     PsrI
     PstI     ScaI     SfcI     SfoI     SmaI     SmlI     SrfI     StyI
    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI
  BsiHKAI    BsiWI    BsmBI    BspHI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     MmeI     MslI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com