Restriction map for
pFN21AB8922
(Circular) (Six-base) MAPSORT of: pFN21AB8922.seq Check: 7027 from: 1 to: 5512
pFN21AB8922 5512 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 2855 1937
Size: 669 249 4594
Fragments arranged by size:
4594 669 249
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 3575 4959 5227 1632
Size: 610 1333 1384 268 1917
Fragments arranged by size:
1917 1384 1333 610 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2624 2656 2624
Size: 32 5480
AlwNI CAG_nnn'CTG
Cuts at: 2623 3255 4827 2623
Size: 632 1572 3308
Fragments arranged by size:
3308 1572 632
ApaI G_GGCC'C
Cuts at: 2596 2596
Size: 5512
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2294 2469 2510 2837 1870
Size: 73 89 262 175 41 327 4545
Fragments arranged by size:
4545 327 262 175 89 73 41
BamHI G'GATC_C
Cuts at: 2110 2842 3209 2110
Size: 732 367 4413
Fragments arranged by size:
4413 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2833 4288 618
Size: 325 217 627 543 503 1455 1842
Fragments arranged by size:
1842 1455 627 543 503 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2222 2253 2487 2596 2831 1391
Size: 621 210 31 234 109 235 4072
Fragments arranged by size:
4072 621 235 234 210 109 31
BbeI G_GCGC'C
Cuts at: 2334 2334
Size: 5512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4541
BbvCI CC'TCA_GC
Cuts at: 2165 2371 2165
Size: 206 5306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2665 2699 3703 3737 1903
Size: 34 728 34 1004 34 3678
Fragments arranged by size:
3678 1004 728 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2515 4123 136
Size: 107 122 71 631 605 843 1608 1525
Fragments arranged by size:
1608 1525 843 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2721 5507 2721
Size: 2786 2726
BlpI GC'TnA_GC
Cuts at: 2450 2929 2450
Size: 479 5033
Bme1580I G_kGCm'C
Cuts at: 1790 2596 3573 4729 1790
Size: 806 977 1156 2573
Fragments arranged by size:
2573 1156 977 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 4201 442
Size: 416 53 83 399 24 1826 958 1753
Fragments arranged by size:
1826 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2365 2645 4172 1239
Size: 937 189 280 1527 2579
Fragments arranged by size:
2579 1527 937 280 189
Bpu10I CC'TnA_GC
Cuts at: 2165 2371 2165
Size: 206 5306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 2720 5506 2720
Size: 2786 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 3319 3701 4336
Size: 53 83 186 936 798 988 382 635
Cuts at: 4336 275
Size: 1451
Fragments arranged by size:
1451 988 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2170 2179 1186
Size: 984 9 4519
Fragments arranged by size:
4519 984 9
BseYI C'CCAG_C
Cuts at: 2459 4715 5211 2459
Size: 2256 496 2760
Fragments arranged by size:
2760 2256 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2293 1163
Size: 573 557 4382
Fragments arranged by size:
4382 573 557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2222 3573 3658 4729 2012
Size: 210 1351 85 1071 2795
Fragments arranged by size:
2795 1351 1071 210 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2222 2253 2487 2596 2831 3573
Size: 399 222 210 31 234 109 235 742
Cuts at: 3573 3658 4729 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 235 234 222 210
109 85 31
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2867 844
Size: 2023 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 2178 3399 1394
Size: 429 274 81 1221 3507
Fragments arranged by size:
3507 1221 429 274 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2736 4156 4343 1700
Size: 110 319 607 1420 187 2869
Fragments arranged by size:
2869 1420 607 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 5213
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2110 2721 2842 3209 3594 3611 5063 5149
Size: 138 611 121 367 385 17 1452 86
Cuts at: 5149 5161 5507 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 611 385 367 346 138 121
86 17 12
BstZ17I GTA'TAC
Cuts at: 2607 2607
Size: 5512
Bsu36I CC'TnA_GG
Cuts at: 2430 2430
Size: 5512
BtgI C'CryG_G
Cuts at: 513 1065 2326 2487 513
Size: 552 1261 161 3538
Fragments arranged by size:
3538 1261 552 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 3028 3822 3842 2086
Size: 942 794 20 3756
Fragments arranged by size:
3756 942 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2168 4519 809
Size: 507 852 2351 1802
Fragments arranged by size:
2351 1802 852 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2959 3848
Size: 54 1425 50 275 18 660 469 889
Cuts at: 3848 5477 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 660 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3442 1353
Size: 2089 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2220 2010
Size: 210 5302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2242 2365 2645 3575 4172 4959
Size: 393 544 66 123 280 930 597 787
Cuts at: 4959 5227 1239
Size: 268 1524
Fragments arranged by size:
1524 930 787 597 544 393 280 268
123 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2542 2542
Size: 5512
EcoO109I rG'GnC_Cy
Cuts at: 2592 2592
Size: 5512
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 2334 2388 2713 2802 4659 1678
Size: 656 54 325 89 1857 2531
Fragments arranged by size:
2531 1857 656 325 89 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2661 2693 4153 4185 4227
Size: 32 135 32 1089 32 1460 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1460 1089 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 2856 3102 669
Size: 988 281 235 683 246 3079
Fragments arranged by size:
3079 988 683 281 246 235
HindIII A'AGCT_T
Cuts at: 748 2549 748
Size: 1801 3711
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KasI G'GCGC_C
Cuts at: 2330 2330
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 4626 4810 1305
Size: 1077 2244 184 2007
Fragments arranged by size:
2244 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 10
Size: 54 1425 343 660 3030
Fragments arranged by size:
3030 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3470 3829 3988 518
Size: 648 2304 359 159 2042
Fragments arranged by size:
2304 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2792 2928 3605 4753 4998 1606
Size: 511 675 136 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 511 245 136
NarI GG'CG_CC
Cuts at: 2331 2331
Size: 5512
NcoI C'CATG_G
Cuts at: 513 1065 2487 513
Size: 552 1422 3538
Fragments arranged by size:
3538 1422 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 1108
Size: 1515 3997
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PspOMI G'GGCC_C
Cuts at: 2592 2592
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
PvuII CAG'CTG
Cuts at: 2792 2792
Size: 5512
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 5512
SacI G_AGCT'C
Cuts at: 2012 2222 2012
Size: 210 5302
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2787 2860 3995 4676 4867 826
Size: 219 1742 73 1135 681 191 1471
Fragments arranged by size:
1742 1471 1135 681 219 191 73
SfoI GGC'GCC
Cuts at: 2332 2332
Size: 5512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2487 2951 513
Size: 552 1422 464 3074
Fragments arranged by size:
3074 1422 552 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI