Restriction map for
pFN21AB8919
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BseRI BsiWI
BsmBI BsmI Bsp1407I BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeIII MfeI MflI MluI MluNI
MscI MseI MslI MspA1I MunI
NdeI NheI NotI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 403 GTCTAC GT^[AC][GT]AC
AccIII 99 TCCGGA T^CCGGA
AlwNI 148 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 470 AGCGCT AGC^GCT
ApaI 220 GGGCCC GGGCC^C
AspHI 9 GAGCTC G[AT]GC[AT]^C
BbsI 730 GAAGAC GAAGAC(2/6)
BclI 2 TGATCA T^GATCA
BfaI 179 CTAG C^TAG
BpuAI 730 GAAGAC GAAGAC(2/6)
BsaWI 99 TCCGGA [AT]^CCGG[AT]
BseAI 99 TCCGGA T^CCGGA
BsgI 204 CTGCAC GTGCAG(16/14)
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
BsmAI 212 GTCTC GTCTC(1/5)
BsmFI 330 GTCCC GGGAC(10/14)
BspDI 37 ATCGAT AT^CGAT
BspEI 99 TCCGGA T^CCGGA
BspHI 5 TCATGA T^CATGA
ClaI 37 ATCGAT AT^CGAT
DraII 219 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 470 AGCGCT AGC^GCT
EcoNI 304 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 219 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 409 CCATGG C^C[AT][AT]GG
FbaI 2 TGATCA T^GATCA
FseI 245 GGCCGGCC GGCCGG^CC
MaeI 179 CTAG C^TAG
MaeII 428 ACGT A^CGT
MroI 99 TCCGGA T^CCGGA
NcoI 409 CCATGG C^CATGG
NruI 232 TCGCGA TCG^CGA
PaeR7I 474 CTCGAG C^TCGAG
PstI 348 CTGCAG CTGCA^G
RcaI 5 TCATGA T^CATGA
SacI 9 GAGCTC GAGCT^C
SexAI 784 ACCAGGT A^CC[AT]GGT
SfaNI 138 GCATC GCATC(5/9)
SfcI 348 CTGCAG C^T[GA][CT]AG
SmaI 717 CCCGGG CCC^GGG
SmlI 474 CTCGAG C^T[CT][GA]AG
StyI 409 CCATGG C^C[AT][AT]GG
XhoI 474 CTCGAG C^TCGAG
XmaCI 717 CCCGGG C^CCGGG
XmaI 717 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 173 CGCG CG^CG
233 CGCG
AcsI 46 AAATTT [GA]^AATT[CT]
422 AAATTC
AcyI 300 GGCGCC G[GA]^CG[CT]C
313 GGCGCC
AfaI 156 GTAC GT^AC
722 GTAC
AlwI 40 GATCC GGATC(4/5)
557 GGATC
ApoI 46 AAATTT [GA]^AATT[CT]
422 AAATTC
AvaII 116 GGACC G^G[AT]CC
639 GGACC
BanI 300 GGCGCC G^G[CT][GA]CC
313 GGCGCC
BanII 9 GAGCTC G[GA]GC[CT]^C
220 GGGCCC
BbeI 300 GGCGCC GGCGC^C
313 GGCGCC
BmyI 9 GAGCTC G[AGT]GC[ACT]^C
220 GGGCCC
BpmI 214 CTCCAG CTGGAG(16/14)
375 CTCCAG
BsaBI 558 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
597 GATCAAAATC
BsaHI 300 GGCGCC G[GA]^CG[CT]C
313 GGCGCC
BsiEI 111 CGGCCG CG[GA][CT]^CG
680 CGGCCG
Bsp1286I 9 GAGCTC G[AGT]GC[ACT]^C
220 GGGCCC
BsrI 195 CCAGT ACTGG(1/-1)
209 CCAGT
BstUI 173 CGCG CG^CG
233 CGCG
DsaI 240 CCACGG C^C[GA][CT]GG
409 CCATGG
EaeI 111 CGGCCG [CT]^GGCC[GA]
680 CGGCCG
EagI 111 CGGCCG C^GGCCG
680 CGGCCG
EclXI 111 CGGCCG C^GGCCG
680 CGGCCG
Eco52I 111 CGGCCG C^GGCCG
680 CGGCCG
FauI 339 CCCGC CCCGC(4/6)
256 GCGGG
Hin1I 300 GGCGCC G[GA]^CG[CT]C
313 GGCGCC
HphI 438 GGTGA GGTGA(8/7)
141 TCACC
KasI 300 GGCGCC G^GCGCC
313 GGCGCC
MamI 558 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
597 GATCAAAATC
MvnI 173 CGCG CG^CG
233 CGCG
NarI 300 GGCGCC GG^CGCC
313 GGCGCC
PleI 25 GACTC GAGTC(4/5)
647 GACTC
RsaI 156 GTAC GT^AC
722 GTAC
SfoI 300 GGCGCC GGC^GCC
313 GGCGCC
SspI 65 AATATT AAT^ATT
104 AATATT
TfiI 124 GAATC G^A[AT]TC
131 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsiYI BslI BsoBI BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI Tsp509I TthHB8I