(Circular) (Six-base) MAPSORT of: pFN21AB8919.seq Check: 2897 from: 1 to: 5497 pFN21AB8919 5497 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 2840 1937 Size: 472 431 4594 Fragments arranged by size: 4594 472 431 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 3560 4944 5212 1632 Size: 1122 806 1384 268 1917 Fragments arranged by size: 1917 1384 1122 806 268 AfeI AGC'GCT Cuts at: 2477 2477 Size: 5497 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2158 3240 4812 2158 Size: 1082 1572 2843 Fragments arranged by size: 2843 1572 1082 ApaI G_GGCC'C Cuts at: 2229 2229 Size: 5497 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2051 2427 2806 3032 3184 5293 1493 Size: 558 376 379 226 152 2109 1697 Fragments arranged by size: 2109 1697 558 379 376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2276 2479 2722 2822 1870 Size: 73 333 203 243 100 4545 Fragments arranged by size: 4545 333 243 203 100 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2318 2818 4273 618 Size: 325 217 627 518 13 500 1455 1842 Fragments arranged by size: 1842 1455 627 518 500 325 217 13 BanII G_rGCy'C Cuts at: 1391 2018 2229 2816 1391 Size: 627 211 587 4072 Fragments arranged by size: 4072 627 587 211 BbeI G_GCGC'C Cuts at: 2309 2322 2309 Size: 13 5484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2742 928 Size: 971 843 3683 Fragments arranged by size: 3683 971 843 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 5038 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4108 136 Size: 107 122 71 631 605 2436 1525 Fragments arranged by size: 2436 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2229 3558 4714 1790 Size: 439 1329 1156 2573 Fragments arranged by size: 2573 1329 1156 439 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2365 4157 1239 Size: 965 161 1792 2579 Fragments arranged by size: 2579 1792 965 161 Bpu10I CC'TnA_GC Cuts at: 2193 2193 Size: 5497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3622 4487 4785 3622 Size: 865 298 4334 Fragments arranged by size: 4334 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 2567 2606 5491 2567 Size: 39 2885 2573 Fragments arranged by size: 2885 2573 39 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2306 2319 3304 3686 Size: 53 83 186 936 773 13 985 382 Cuts at: 3686 4321 275 Size: 635 1451 Fragments arranged by size: 1451 985 936 773 635 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 2104 3930 4328 4602 4749 5160 2104 Size: 1826 398 274 147 411 2441 Fragments arranged by size: 2441 1826 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 1178 Size: 30 165 30 960 30 4282 Fragments arranged by size: 4282 960 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5497 BseYI C'CCAG_C Cuts at: 2338 2699 4700 5196 2338 Size: 361 2001 496 2639 Fragments arranged by size: 2639 2001 496 361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2194 1163 Size: 573 458 4466 Fragments arranged by size: 4466 573 458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2119 2688 2947 3708 4736 1540 Size: 275 186 118 569 259 761 1028 2301 Fragments arranged by size: 2301 1028 761 569 275 259 186 118 BsiHKAI G_wGCw'C Cuts at: 2018 3558 3643 4714 2018 Size: 1540 85 1071 2801 Fragments arranged by size: 2801 1540 1071 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2229 2816 3558 3643 4714 1391 Size: 399 228 211 587 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 587 399 228 211 85 BspEI T'CCGG_A Cuts at: 2104 5160 2104 Size: 3056 2441 BspHI T'CATG_A Cuts at: 2010 3386 5116 2010 Size: 1376 1730 2391 Fragments arranged by size: 2391 1730 1376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 844 Size: 2008 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2251 2302 2687 4141 4328 1700 Size: 110 441 51 385 1454 187 2869 Fragments arranged by size: 2869 1454 441 385 187 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492 Size: 855 367 385 17 1452 86 12 346 Cuts at: 5492 1972 Size: 1977 Fragments arranged by size: 1977 1452 855 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2245 2414 513 Size: 552 1180 169 3596 Fragments arranged by size: 3596 1180 552 169 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 3013 3807 3827 3013 Size: 794 20 4683 Fragments arranged by size: 4683 794 20 ClaI AT'CG_AT Cuts at: 1554 2043 1554 Size: 489 5008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2685 2944 Size: 54 1425 50 275 18 286 569 259 Cuts at: 2944 3833 5462 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 569 286 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2116 2685 2944 1537 Size: 275 304 569 259 4090 Fragments arranged by size: 4090 569 304 275 259 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2314 4084 4458 4604 387 Size: 1064 138 725 1770 374 146 1280 Fragments arranged by size: 1770 1280 1064 725 374 146 138 EcoICRI GAG'CTC Cuts at: 2016 2016 Size: 5497 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2365 2754 3560 4157 4944 5212 Size: 393 572 161 389 806 597 787 268 Cuts at: 5212 1239 Size: 1524 Fragments arranged by size: 1524 806 787 597 572 393 389 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2313 2313 Size: 5497 EcoO109I rG'GnC_Cy Cuts at: 2225 2225 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FseI GG_CCGG'CC Cuts at: 2255 2255 Size: 5497 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2200 2309 2322 2388 2479 2694 2754 4644 Size: 522 109 13 66 91 215 60 1890 Cuts at: 4644 1678 Size: 2531 Fragments arranged by size: 2531 1890 522 215 109 91 66 60 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 4138 4170 4212 Size: 32 135 32 791 32 1743 32 42 Cuts at: 4212 4244 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 791 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KasI G'GCGC_C Cuts at: 2305 2318 2305 Size: 13 5484 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4611 4795 1305 Size: 3306 184 2007 Fragments arranged by size: 3306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3455 3814 3973 518 Size: 648 843 1446 359 159 2042 Fragments arranged by size: 2042 1446 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2913 3590 4738 4983 1606 Size: 1307 677 1148 245 2120 Fragments arranged by size: 2120 1307 1148 677 245 NaeI GCC'GGC Cuts at: 2253 2304 2689 2253 Size: 51 385 5061 Fragments arranged by size: 5061 385 51 NarI GG'CG_CC Cuts at: 2306 2319 2306 Size: 13 5484 NcoI C'CATG_G Cuts at: 513 1065 2414 513 Size: 552 1349 3596 Fragments arranged by size: 3596 1349 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NgoMIV G'CCGG_C Cuts at: 2251 2302 2687 2251 Size: 51 385 5061 Fragments arranged by size: 5061 385 51 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NruI TCG'CGA Cuts at: 2239 2239 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PfoI T'CCnGG_A Cuts at: 2508 2508 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 2457 3067 2457 Size: 610 4887 PspOMI G'GGCC_C Cuts at: 2225 2225 Size: 5497 PspXI vC'TCGA_Gb Cuts at: 2479 2479 Size: 5497 PstI C_TGCA'G Cuts at: 830 2357 2849 830 Size: 1527 492 3478 Fragments arranged by size: 3478 1527 492 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SacI G_AGCT'C Cuts at: 2018 2018 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SexAI A'CCwGG_T Cuts at: 2789 2789 Size: 5497 SfcI C'TryA_G Cuts at: 826 1045 2353 2845 3980 4661 4852 826 Size: 219 1308 492 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 681 492 219 191 SfoI GGC'GCC Cuts at: 2307 2320 2307 Size: 13 5484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2724 2824 1872 Size: 852 100 4545 Fragments arranged by size: 4545 852 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 2479 3601 4502 4764 820 Size: 197 926 536 1122 901 262 1553 Fragments arranged by size: 1553 1122 926 901 536 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 2072 2111 3421 5 Size: 47 2020 39 1310 2081 Fragments arranged by size: 2081 2020 1310 47 39 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2414 2936 513 Size: 552 1349 522 3074 Fragments arranged by size: 3074 1349 552 522 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3524 3541 3694 3879 4324 325 Size: 1255 1944 17 153 185 445 1498 Fragments arranged by size: 1944 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 2479 1943 Size: 536 4961 XmaI C'CCGG_G Cuts at: 1870 2722 2822 1870 Size: 852 100 4545 Fragments arranged by size: 4545 852 100 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI