Restriction map for
pFN21AB8919
(Linear) (Six-base) MAPSORT of: KIBB8919.seq Check: 5740 from: 1 to: 792
KIBB8919.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 404 792
Size: 404 388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 792
Size: 749 43
AfeI AGC'GCT
Cuts at: 0 472 792
Size: 472 320
AlwNI CAG_nnn'CTG
Cuts at: 0 153 792
Size: 153 639
ApaI G_GGCC'C
Cuts at: 0 224 792
Size: 224 568
ApoI r'AATT_y
Cuts at: 0 46 422 792
Size: 46 376 370
Fragments arranged by size:
376 370 46
AvaI C'yCGr_G
Cuts at: 0 271 474 717 792
Size: 271 203 243 75
Fragments arranged by size:
271 243 203 75
BanI G'GyrC_C
Cuts at: 0 300 313 792
Size: 300 13 479
Fragments arranged by size:
479 300 13
BanII G_rGCy'C
Cuts at: 0 13 224 792
Size: 13 211 568
Fragments arranged by size:
568 211 13
BbeI G_GCGC'C
Cuts at: 0 304 317 792
Size: 304 13 475
Fragments arranged by size:
475 304 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 737 792
Size: 737 55
BclI T'GATC_A
Cuts at: 0 2 792
Size: 2 790
Bme1580I G_kGCm'C
Cuts at: 0 224 792
Size: 224 568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 360 792
Size: 199 161 432
Fragments arranged by size:
432 199 161
Bpu10I CC'TnA_GC
Cuts at: 0 188 792
Size: 188 604
BsaBI GATnn'nnATC
Cuts at: 0 562 601 792
Size: 562 39 191
Fragments arranged by size:
562 191 39
BsaHI Gr'CG_yC
Cuts at: 0 301 314 792
Size: 301 13 478
Fragments arranged by size:
478 301 13
BsaWI w'CCGG_w
Cuts at: 0 99 792
Size: 99 693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 792
Size: 358 30 404
Fragments arranged by size:
404 358 30
BseYI C'CCAG_C
Cuts at: 0 333 694 792
Size: 333 361 98
Fragments arranged by size:
361 333 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 792
Size: 189 603
BsiEI CG_ry'CG
Cuts at: 0 114 683 792
Size: 114 569 109
Fragments arranged by size:
569 114 109
BsiHKAI G_wGCw'C
Cuts at: 0 13 792
Size: 13 779
Bsp1286I G_dGCh'C
Cuts at: 0 13 224 792
Size: 13 211 568
Fragments arranged by size:
568 211 13
BspEI T'CCGG_A
Cuts at: 0 99 792
Size: 99 693
BspHI T'CATG_A
Cuts at: 0 5 792
Size: 5 787
BsrFI r'CCGG_y
Cuts at: 0 246 297 682 792
Size: 246 51 385 110
Fragments arranged by size:
385 246 110 51
BtgI C'CryG_G
Cuts at: 0 240 409 792
Size: 240 169 383
Fragments arranged by size:
383 240 169
ClaI AT'CG_AT
Cuts at: 0 38 792
Size: 38 754
EaeI y'GGCC_r
Cuts at: 0 111 680 792
Size: 111 569 112
Fragments arranged by size:
569 112 111
EagI C'GGCC_G
Cuts at: 0 111 680 792
Size: 111 569 112
Fragments arranged by size:
569 112 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 309 792
Size: 309 483
EcoICRI GAG'CTC
Cuts at: 0 11 792
Size: 11 781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 360 749 792
Size: 199 161 389 43
Fragments arranged by size:
389 199 161 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 308 792
Size: 308 484
EcoO109I rG'GnC_Cy
Cuts at: 0 220 792
Size: 220 572
FseI GG_CCGG'CC
Cuts at: 0 250 792
Size: 250 542
HaeII r_GCGC'y
Cuts at: 0 195 304 317 383 474 689 749 792
Size: 195 109 13 66 91 215 60 43
Fragments arranged by size:
215 195 109 91 66 60 43 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 792
Size: 358 32 402
Fragments arranged by size:
402 358 32
KasI G'GCGC_C
Cuts at: 0 300 313 792
Size: 300 13 479
Fragments arranged by size:
479 300 13
NaeI GCC'GGC
Cuts at: 0 248 299 684 792
Size: 248 51 385 108
Fragments arranged by size:
385 248 108 51
NarI GG'CG_CC
Cuts at: 0 301 314 792
Size: 301 13 478
Fragments arranged by size:
478 301 13
NcoI C'CATG_G
Cuts at: 0 409 792
Size: 409 383
NgoMIV G'CCGG_C
Cuts at: 0 246 297 682 792
Size: 246 51 385 110
Fragments arranged by size:
385 246 110 51
NruI TCG'CGA
Cuts at: 0 234 792
Size: 234 558
PfoI T'CCnGG_A
Cuts at: 0 503 792
Size: 503 289
PsiI TTA'TAA
Cuts at: 0 452 792
Size: 452 340
PspOMI G'GGCC_C
Cuts at: 0 220 792
Size: 220 572
PspXI vC'TCGA_Gb
Cuts at: 0 474 792
Size: 474 318
PstI C_TGCA'G
Cuts at: 0 352 792
Size: 352 440
SacI G_AGCT'C
Cuts at: 0 13 792
Size: 13 779
SexAI A'CCwGG_T
Cuts at: 0 784 792
Size: 784 8
SfcI C'TryA_G
Cuts at: 0 348 792
Size: 348 444
SfoI GGC'GCC
Cuts at: 0 302 315 792
Size: 302 13 477
Fragments arranged by size:
477 302 13
SmaI CCC'GGG
Cuts at: 0 719 792
Size: 719 73
SmlI C'TyrA_G
Cuts at: 0 474 792
Size: 474 318
SspI AAT'ATT
Cuts at: 0 67 106 792
Size: 67 39 686
Fragments arranged by size:
686 67 39
StyI C'CwwG_G
Cuts at: 0 409 792
Size: 409 383
XhoI C'TCGA_G
Cuts at: 0 474 792
Size: 474 318
XmaI C'CCGG_G
Cuts at: 0 717 792
Size: 717 75
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BclI Bme1580I BpmI Bpu10I BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BsrFI BtgI ClaI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI
NaeI NarI NcoI NgoMIV NruI PfoI PsiI PspOMI
PspXI PstI SacI SexAI SfcI SfoI SmaI SmlI
SspI StyI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BseRI BsiWI BsmI BsmBI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EarI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI