Restriction map for
pFN21AB8917
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiHKAI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MunI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp509I Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 422 ACATGT A^C[GA][CT]GT
Aor51HI 584 AGCGCT AGC^GCT
ApaI 13 GGGCCC GGGCC^C
BalI 548 TGGCCA TGG^CCA
BbsI 61 GAAGAC GAAGAC(2/6)
BfaI 70 CTAG C^TAG
BglI 88 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 619 CTGGAG CTGGAG(16/14)
BpuAI 61 GAAGAC GAAGAC(2/6)
BsaAI 31 TACGTG [CT]AC^GT[GA]
BseRI 514 GAGGAG GAGGAG(10/8)
BsiWI 236 CGTACG C^GTACG
BsmAI 310 GTCTC GTCTC(1/5)
BsmBI 309 CGTCTC CGTCTC(1/5)
BsmFI 656 GGGAC GGGAC(10/14)
BspLU11I 422 ACATGT A^CATGT
BspMI 702 GCAGGT ACCTGC(4/8)
BssSI 573 CACGAG CACGAG(-5/-1)
BstAPI 462 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 119 CCAGCGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 212 CTCAG C^T[ACGT]AG
DraII 12 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 189 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 329 CGGCCG C^GGCCG
EarI 634 CTCTTC CTCTTC(1/4)
EclXI 329 CGGCCG C^GGCCG
Eco47III 584 AGCGCT AGC^GCT
Eco52I 329 CGGCCG C^GGCCG
EcoO109I 12 GGGGCCC [GA]G^G[ACGT]CC[CT]
Ksp632I 634 CTCTTC CTCTTC(1/4)
MaeI 70 CTAG C^TAG
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MseI 649 TTAA T^TAA
MslI 52 CACGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 289 CTCGAG C^TCGAG
PflMI 606 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 37 GACTC GAGTC(4/5)
Tru9I 649 TTAA T^TAA
Tsp45I 341 GTCAC ^GT[GC]AC
Van91I 606 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 536 CCAGCAACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 289 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 463 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
607 CAGACGCTG
AvaII 531 GGACC G^G[AT]CC
657 GGACC
BanII 13 GGGCCC G[GA]GC[CT]^C
95 GGGCTC
BsiEI 329 CGGCCG CG[GA][CT]^CG
450 CGACCG
BsrBI 567 CCGCTC CCGCTC(-3/-3)
697 GAGCGG
BsrFI 174 GCCGGC [GA]^CCGG[CT]
496 GCCGGC
BsrI 554 ACTGG ACTGG(1/-1)
618 ACTGG
BstF5I 100 CATCC GGATG(2/0)
731 GGATG
Cfr10I 174 GCCGGC [GA]^CCGG[CT]
496 GCCGGC
DsaI 221 CCGTGG C^C[GA][CT]GG
545 CCGTGG
FokI 100 CATCC GGATG(9/13)
731 GGATG
HphI 231 TCACC GGTGA(8/7)
692 TCACC
MaeII 32 ACGT A^CGT
190 ACGT
MaeIII 23 GTAAC ^GT[ACGT]AC
341 GTCAC
MspA1I 120 CAGCGG C[AC]G^C[GT]G
295 CCGCTG
NaeI 174 GCCGGC GCC^GGC
496 GCCGGC
NgoMIV 174 GCCGGC G^CCGGC
496 GCCGGC
NlaIII 276 CATG CATG^
423 CATG
NspI 275 ACATGC [GA]CATG^[CT]
422 ACATGT
SfcI 138 CTGTAG C^T[GA][CT]AG
156 CTGTAG
SmlI 289 CTCGAG C^T[CT][GA]AG
352 CTCAAG
TaqI 290 TCGA T^CGA
726 TCGA
TfiI 558 GATTC G^A[AT]TC
723 GAATC
TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CACTG
TthHB8I 290 TCGA T^CGA
726 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MboII MnlI
MspI MvaI MvnI MwoI NarI
NciI NlaIV RsaI Sau96I ScrFI
SfoI SmaI TseI XmaCI XmaI