(Circular) (Six-base) MAPSORT of: pFN21AB8917.seq Check: 9577 from: 1 to: 5485 pFN21AB8917 5485 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4932 5200 1632 Size: 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 268 AfeI AGC'GCT Cuts at: 2591 2591 Size: 5485 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 2427 4384 5361 1203 Size: 1224 1957 977 1327 Fragments arranged by size: 1957 1327 1224 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 2473 2617 3228 4800 2473 Size: 144 611 1572 3158 Fragments arranged by size: 3158 1572 611 144 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 5485 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2794 3020 3172 5281 1493 Size: 1301 226 152 2109 1697 Fragments arranged by size: 2109 1697 1301 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2294 2375 2441 2483 2682 2810 1870 Size: 73 351 81 66 42 199 128 4545 Fragments arranged by size: 4545 351 199 128 81 73 66 42 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2203 2264 2309 2379 Size: 325 217 627 347 69 61 45 70 Cuts at: 2379 2806 4261 618 Size: 427 1455 1842 Fragments arranged by size: 1842 1455 627 427 347 325 217 70 69 61 45 BanII G_rGCy'C Cuts at: 1391 2022 2104 2804 1391 Size: 631 82 700 4072 Fragments arranged by size: 4072 700 631 82 BbeI G_GCGC'C Cuts at: 2207 2268 2313 2383 2207 Size: 61 45 70 5309 Fragments arranged by size: 5309 70 61 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 928 Size: 971 174 4340 Fragments arranged by size: 4340 971 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2099 4096 Size: 107 122 71 631 605 337 90 1997 Cuts at: 4096 136 Size: 1525 Fragments arranged by size: 1997 1525 631 605 337 122 107 90 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2022 2214 3546 4702 1790 Size: 232 192 1332 1156 2573 Fragments arranged by size: 2573 1332 1156 232 192 BmgBI CAC'GTC Cuts at: 1318 2196 1318 Size: 878 4607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4174 442 Size: 416 53 83 399 24 1799 958 1753 Fragments arranged by size: 1799 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2645 4145 1239 Size: 1406 1500 2579 Fragments arranged by size: 2579 1500 1406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 3610 4475 4773 2342 Size: 1268 865 298 3054 Fragments arranged by size: 3054 1268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 3276 493 Size: 711 834 1238 2702 Fragments arranged by size: 2702 1238 834 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 2265 2310 2367 Size: 53 83 186 936 671 61 45 57 Cuts at: 2367 2380 3292 3674 4309 275 Size: 13 912 382 635 1451 Fragments arranged by size: 1451 936 912 671 635 382 186 83 61 57 53 45 13 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2558 2588 2618 2648 1178 Size: 30 165 30 1155 30 30 30 4015 Fragments arranged by size: 4015 1155 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2534 1186 Size: 1348 4137 BseYI C'CCAG_C Cuts at: 2123 2250 2324 2540 4688 5184 2123 Size: 127 74 216 2148 496 2424 Fragments arranged by size: 2424 2148 496 216 127 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4912 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 2458 2935 3696 4724 1540 Size: 275 186 336 121 477 761 1028 2301 Fragments arranged by size: 2301 1028 761 477 336 275 186 121 BsiHKAI G_wGCw'C Cuts at: 3546 3631 4702 3546 Size: 85 1071 4329 Fragments arranged by size: 4329 1071 85 BsiWI C'GTAC_G Cuts at: 2241 2241 Size: 5485 BsmI GAATG_Cn' Cuts at: 2996 3089 2996 Size: 93 5392 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 2320 Size: 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2104 2214 2804 3546 3631 4702 Size: 399 232 82 110 590 742 85 1071 Cuts at: 4702 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 590 399 232 110 85 82 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2698 2840 844 Size: 1854 142 3489 Fragments arranged by size: 3489 1854 142 BsrBI CCG'CTC Cuts at: 1394 1823 2574 2704 3372 1394 Size: 429 751 130 668 3507 Fragments arranged by size: 3507 751 668 429 130 BsrDI GCAATG_nn' Cuts at: 65 1248 3980 4154 65 Size: 1183 2732 174 1396 Fragments arranged by size: 2732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2179 2501 4129 4316 1700 Size: 110 369 322 1628 187 2869 Fragments arranged by size: 2869 1628 369 322 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 2578 3545 4557 2578 Size: 967 1012 3506 Fragments arranged by size: 3506 1012 967 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 3806 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2131 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2226 2550 513 Size: 552 1161 324 3448 Fragments arranged by size: 3448 1161 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 2190 2471 3001 3795 3815 2190 Size: 281 530 794 20 3860 Fragments arranged by size: 3860 794 530 281 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DraIII CAC_nnn'GTG Cuts at: 2199 2199 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2334 2553 Size: 54 1425 50 275 18 261 243 219 Cuts at: 2553 2932 3821 5450 8 Size: 379 889 1629 43 Fragments arranged by size: 1629 1425 889 379 275 261 243 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2334 2932 1537 Size: 275 522 598 4090 Fragments arranged by size: 4090 598 522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 3415 1353 Size: 1292 770 3423 Fragments arranged by size: 3423 1292 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2504 4072 4446 4592 387 Size: 1064 138 915 1568 374 146 1280 Fragments arranged by size: 1568 1280 1064 915 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2645 3548 4145 4932 5200 1239 Size: 393 1013 903 597 787 268 1524 Fragments arranged by size: 1524 1013 903 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2018 2018 Size: 5485 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 2207 2268 2313 2383 2593 4632 1678 Size: 529 61 45 70 210 2039 2531 Fragments arranged by size: 2531 2039 529 210 70 61 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2288 2320 4126 Size: 32 135 32 569 32 115 32 1806 Cuts at: 4126 4158 4200 4232 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1806 569 135 115 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KasI G'GCGC_C Cuts at: 2203 2264 2309 2379 2203 Size: 61 45 70 5309 Fragments arranged by size: 5309 70 61 45 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 10 Size: 54 1425 343 723 2940 Fragments arranged by size: 2940 1425 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 3443 3802 3961 518 Size: 648 895 1382 359 159 2042 Fragments arranged by size: 2042 1382 895 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2127 2302 2901 3578 4726 4971 1606 Size: 521 175 599 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 599 521 245 175 NaeI GCC'GGC Cuts at: 2181 2503 2181 Size: 322 5163 NarI GG'CG_CC Cuts at: 2204 2265 2310 2380 2204 Size: 61 45 70 5309 Fragments arranged by size: 5309 70 61 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NgoMIV G'CCGG_C Cuts at: 2179 2501 2179 Size: 322 5163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2284 2431 2843 4388 1132 Size: 97 1055 147 412 1545 2229 Fragments arranged by size: 2229 1545 1055 412 147 97 PasI CC'CwG_GG Cuts at: 1348 2399 1348 Size: 1051 4434 PciI A'CATG_T Cuts at: 2427 4384 2427 Size: 1957 3528 PflMI CCAn_nnn'nTGG Cuts at: 1108 2617 1108 Size: 1509 3976 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 5485 PspXI vC'TCGA_Gb Cuts at: 2294 2294 Size: 5485 PstI C_TGCA'G Cuts at: 830 2837 830 Size: 2007 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2143 2161 2833 3968 4649 4840 826 Size: 219 1098 18 672 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 681 672 219 191 18 SfoI GGC'GCC Cuts at: 2205 2266 2311 2381 2205 Size: 61 45 70 5309 Fragments arranged by size: 5309 70 61 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2377 2443 2684 2812 1872 Size: 505 66 241 128 4545 Fragments arranged by size: 4545 505 241 128 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 2357 3589 4490 4752 820 Size: 197 926 351 63 1232 901 262 1553 Fragments arranged by size: 1553 1232 926 901 351 262 197 63 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2924 513 Size: 552 1859 3074 Fragments arranged by size: 3074 1859 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3731 5443 96 Size: 274 80 33 51 1443 1754 1712 138 Fragments arranged by size: 1754 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 5445 5477 2558 Size: 32 2855 32 2566 Fragments arranged by size: 2855 2566 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2548 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 2294 1943 Size: 351 5134 XmaI C'CCGG_G Cuts at: 1870 2375 2441 2682 2810 1870 Size: 505 66 241 128 4545 Fragments arranged by size: 4545 505 241 128 66 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI