Restriction map for
pFN21AB8917
(Linear) (Six-base) MAPSORT of: KIBB8917.seq Check: 1060 from: 1 to: 780
KIBB8917.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AfeI AGC'GCT
Cuts at: 0 586 780
Size: 586 194
AflIII A'CryG_T
Cuts at: 0 422 780
Size: 422 358
AlwNI CAG_nnn'CTG
Cuts at: 0 468 612 780
Size: 468 144 168
Fragments arranged by size:
468 168 144
ApaI G_GGCC'C
Cuts at: 0 17 780
Size: 17 763
AvaI C'yCGr_G
Cuts at: 0 289 370 436 478 677 780
Size: 289 81 66 42 199 103
Fragments arranged by size:
289 199 103 81 66 42
BanI G'GyrC_C
Cuts at: 0 129 198 259 304 374 780
Size: 129 69 61 45 70 406
Fragments arranged by size:
406 129 70 69 61 45
BanII G_rGCy'C
Cuts at: 0 17 99 780
Size: 17 82 681
Fragments arranged by size:
681 82 17
BbeI G_GCGC'C
Cuts at: 0 202 263 308 378 780
Size: 202 61 45 70 402
Fragments arranged by size:
402 202 70 61 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 68 780
Size: 68 712
BglI GCCn_nnn'nGGC
Cuts at: 0 94 780
Size: 94 686
Bme1580I G_kGCm'C
Cuts at: 0 17 209 780
Size: 17 192 571
Fragments arranged by size:
571 192 17
BmgBI CAC'GTC
Cuts at: 0 191 780
Size: 191 589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 780
Size: 640 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 780
Size: 337 443
BsaAI yAC'GTr
Cuts at: 0 33 780
Size: 33 747
BsaHI Gr'CG_yC
Cuts at: 0 199 260 305 362 375 780
Size: 199 61 45 57 13 405
Fragments arranged by size:
405 199 61 57 45 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 613 643 780
Size: 553 30 30 30 137
Fragments arranged by size:
553 137 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 780
Size: 529 251
BseYI C'CCAG_C
Cuts at: 0 118 245 319 535 780
Size: 118 127 74 216 245
Fragments arranged by size:
245 216 127 118 74
BsiEI CG_ry'CG
Cuts at: 0 332 453 780
Size: 332 121 327
Fragments arranged by size:
332 327 121
BsiWI C'GTAC_G
Cuts at: 0 236 780
Size: 236 544
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 780
Size: 315 465
Bsp1286I G_dGCh'C
Cuts at: 0 17 99 209 780
Size: 17 82 110 571
Fragments arranged by size:
571 110 82 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 693 780
Size: 693 87
BsrBI CCG'CTC
Cuts at: 0 569 699 780
Size: 569 130 81
Fragments arranged by size:
569 130 81
BsrFI r'CCGG_y
Cuts at: 0 174 496 780
Size: 174 322 284
Fragments arranged by size:
322 284 174
BssSI C'ACGA_G
Cuts at: 0 573 780
Size: 573 207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 780
Size: 468 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 780
Size: 126 654
BtgI C'CryG_G
Cuts at: 0 221 545 780
Size: 221 324 235
Fragments arranged by size:
324 235 221
BtsI GCAGTG_nn'
Cuts at: 0 185 466 780
Size: 185 281 314
Fragments arranged by size:
314 281 185
DraIII CAC_nnn'GTG
Cuts at: 0 194 780
Size: 194 586
EaeI y'GGCC_r
Cuts at: 0 86 329 548 780
Size: 86 243 219 232
Fragments arranged by size:
243 232 219 86
EagI C'GGCC_G
Cuts at: 0 329 780
Size: 329 451
EarI CTCTTCn'nnn_
Cuts at: 0 640 780
Size: 640 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 499 780
Size: 499 281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 780
Size: 640 140
EcoO109I rG'GnC_Cy
Cuts at: 0 13 780
Size: 13 767
HaeII r_GCGC'y
Cuts at: 0 202 263 308 378 588 780
Size: 202 61 45 70 210 192
Fragments arranged by size:
210 202 192 70 61 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 283 315 780
Size: 136 32 115 32 465
Fragments arranged by size:
465 136 115 32 32
KasI G'GCGC_C
Cuts at: 0 198 259 304 374 780
Size: 198 61 45 70 406
Fragments arranged by size:
406 198 70 61 45
MscI TGG'CCA
Cuts at: 0 550 780
Size: 550 230
MslI CAynn'nnrTG
Cuts at: 0 56 780
Size: 56 724
MspA1I CmG'CkG
Cuts at: 0 122 297 780
Size: 122 175 483
Fragments arranged by size:
483 175 122
NaeI GCC'GGC
Cuts at: 0 176 498 780
Size: 176 322 282
Fragments arranged by size:
322 282 176
NarI GG'CG_CC
Cuts at: 0 199 260 305 375 780
Size: 199 61 45 70 405
Fragments arranged by size:
405 199 70 61 45
NgoMIV G'CCGG_C
Cuts at: 0 174 496 780
Size: 174 322 284
Fragments arranged by size:
322 284 174
NspI r_CATG'y
Cuts at: 0 279 426 780
Size: 279 147 354
Fragments arranged by size:
354 279 147
PasI CC'CwG_GG
Cuts at: 0 394 780
Size: 394 386
PciI A'CATG_T
Cuts at: 0 422 780
Size: 422 358
PflMI CCAn_nnn'nTGG
Cuts at: 0 612 780
Size: 612 168
PspOMI G'GGCC_C
Cuts at: 0 13 780
Size: 13 767
PspXI vC'TCGA_Gb
Cuts at: 0 289 780
Size: 289 491
SfcI C'TryA_G
Cuts at: 0 138 156 780
Size: 138 18 624
Fragments arranged by size:
624 138 18
SfoI GGC'GCC
Cuts at: 0 200 261 306 376 780
Size: 200 61 45 70 404
Fragments arranged by size:
404 200 70 61 45
SmaI CCC'GGG
Cuts at: 0 372 438 679 780
Size: 372 66 241 101
Fragments arranged by size:
372 241 101 66
SmlI C'TyrA_G
Cuts at: 0 289 352 780
Size: 289 63 428
Fragments arranged by size:
428 289 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 780
Size: 553 32 195
Fragments arranged by size:
553 195 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 780
Size: 543 237
XhoI C'TCGA_G
Cuts at: 0 289 780
Size: 289 491
XmaI C'CCGG_G
Cuts at: 0 370 436 677 780
Size: 370 66 241 103
Fragments arranged by size:
370 241 103 66
Enzymes that do cut:
AfeI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BpmI BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstXI BtgI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PspOMI PspXI SfcI SfoI SmaI
SmlI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BsgI BsiHKAI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XmnI ZraI