(Circular) (Six-base) MAPSORT of: pFN21AB8914.seq Check: 1045 from: 1 to: 5470 pFN21AB8914 5470 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3280 278 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2403 2813 1937 Size: 466 410 4594 Fragments arranged by size: 4594 466 410 Acc65I G'GTAC_C Cuts at: 2791 2791 Size: 5470 AclI AA'CG_TT Cuts at: 1563 3597 3970 1563 Size: 2034 373 3063 Fragments arranged by size: 3063 2034 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3533 4917 5185 1632 Size: 1901 1384 268 1917 Fragments arranged by size: 1917 1901 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5273 AflIII A'CryG_T Cuts at: 1203 4369 5346 1203 Size: 3166 977 1327 Fragments arranged by size: 3166 1327 977 AgeI A'CCGG_T Cuts at: 2453 4301 2453 Size: 1848 3622 AhdI GACnn_n'nnGTC Cuts at: 4199 4199 Size: 5470 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5470 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5470 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2036 Size: 32 5438 AlwNI CAG_nnn'CTG Cuts at: 3213 4785 3213 Size: 1572 3898 ApaLI G'TGCA_C Cuts at: 2423 3527 4683 2423 Size: 1104 1156 3210 Fragments arranged by size: 3210 1156 1104 ApoI r'AATT_y Cuts at: 1493 2431 2519 2779 3005 3157 5266 1493 Size: 938 88 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 260 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4024 160 Size: 872 2992 1606 Fragments arranged by size: 2992 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 AvaI C'yCGr_G Cuts at: 1870 1943 2149 2204 2334 2372 2795 1870 Size: 73 206 55 130 38 423 4545 Fragments arranged by size: 4545 423 206 130 73 55 38 AvrII C'CTAG_G Cuts at: 2089 2089 Size: 5470 BamHI G'GATC_C Cuts at: 2800 3167 2800 Size: 367 5103 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2305 2317 2330 2547 Size: 325 217 627 315 203 12 13 217 Cuts at: 2547 2791 4246 618 Size: 244 1455 1842 Fragments arranged by size: 1842 1455 627 325 315 244 217 217 203 13 12 BanII G_rGCy'C Cuts at: 1391 2157 2789 1391 Size: 766 632 4072 Fragments arranged by size: 4072 766 632 BbeI G_GCGC'C Cuts at: 2106 2309 2321 2334 2551 2106 Size: 203 12 13 217 5025 Fragments arranged by size: 5025 217 203 13 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4499 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3661 3695 1903 Size: 34 1724 34 3678 Fragments arranged by size: 3678 1724 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3362 4572 3362 Size: 1210 4260 BclI T'GATC_A Cuts at: 1548 1548 Size: 5470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2152 4081 136 Size: 107 122 71 631 605 480 1929 1525 Fragments arranged by size: 1929 1525 631 605 480 122 107 71 BglII A'GATC_T Cuts at: 2601 5465 2601 Size: 2864 2606 BlpI GC'TnA_GC Cuts at: 2887 2887 Size: 5470 Bme1580I G_kGCm'C Cuts at: 1790 2427 3531 4687 1790 Size: 637 1104 1156 2573 Fragments arranged by size: 2573 1156 1104 637 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3201 4159 442 Size: 416 53 83 399 24 1784 958 1753 Fragments arranged by size: 1784 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 4130 1239 Size: 1023 1868 2579 Fragments arranged by size: 2579 1868 1023 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3595 4460 4758 2369 Size: 1226 865 298 3081 Fragments arranged by size: 3081 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4133 882 Size: 617 2634 2219 Fragments arranged by size: 2634 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2428 3261 493 Size: 711 1224 833 2702 Fragments arranged by size: 2702 1224 833 711 BsaBI GATnn'nnATC Cuts at: 2561 2600 5464 2561 Size: 39 2864 2567 Fragments arranged by size: 2864 2567 39 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2306 2318 2331 Size: 53 83 186 936 570 203 12 13 Cuts at: 2331 2548 3277 3659 4294 275 Size: 217 729 382 635 1451 Fragments arranged by size: 1451 936 729 635 570 382 217 203 186 83 53 13 12 BsaWI w'CCGG_w Cuts at: 2453 2616 3903 4301 4575 4722 5133 2453 Size: 163 1287 398 274 147 411 2790 Fragments arranged by size: 2790 1287 411 398 274 163 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5245 Fragments arranged by size: 5245 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2677 2689 2692 1186 Size: 895 596 12 3 3964 Fragments arranged by size: 3964 895 596 12 3 BseYI C'CCAG_C Cuts at: 2293 2714 4673 5169 2293 Size: 421 1959 496 2594 Fragments arranged by size: 2594 1959 496 421 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4897 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2397 2920 3681 4709 1540 Size: 275 186 396 523 761 1028 2301 Fragments arranged by size: 2301 1028 761 523 396 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3531 3616 4687 2427 Size: 1104 85 1071 3210 Fragments arranged by size: 3210 1104 1071 85 BsmI GAATG_Cn' Cuts at: 2981 3074 2981 Size: 93 5377 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2427 2789 3531 3616 4687 1391 Size: 399 367 270 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 362 270 85 BspEI T'CCGG_A Cuts at: 5133 5133 Size: 5470 BspHI T'CATG_A Cuts at: 2010 3359 5089 2010 Size: 1349 1730 2391 Fragments arranged by size: 2391 1730 1349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 2825 844 Size: 1811 170 3489 Fragments arranged by size: 3489 1811 170 BsrBI CCG'CTC Cuts at: 1394 1823 2276 3357 1394 Size: 429 453 1081 3507 Fragments arranged by size: 3507 1081 453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3965 4139 65 Size: 1183 2717 174 1396 Fragments arranged by size: 2717 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2314 2392 2453 4114 4301 1700 Size: 110 504 78 61 1661 187 2869 Fragments arranged by size: 2869 1661 504 187 110 78 61 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5470 BssHII G'CGCG_C Cuts at: 1925 2124 1925 Size: 199 5271 BssSI C'ACGA_G Cuts at: 3530 4542 3530 Size: 1012 4458 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5470 BstBI TT'CG_AA Cuts at: 4274 4274 Size: 5470 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5470 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2041 Size: 5470 BstYI r'GATC_y Cuts at: 1972 2601 2800 3167 3552 3569 5021 5107 5119 Size: 629 199 367 385 17 1452 86 12 Cuts at: 5119 5465 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 629 385 367 346 199 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2162 2397 513 Size: 552 939 158 235 3586 Fragments arranged by size: 3586 939 552 235 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4731 Fragments arranged by size: 4731 683 56 BtsI GCAGTG_nn' Cuts at: 2986 3780 3800 2986 Size: 794 20 4656 Fragments arranged by size: 4656 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5435 DraI TTT'AAA Cuts at: 2774 2774 Size: 5470 DraIII CAC_nnn'GTG Cuts at: 2577 2577 Size: 5470 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4477 809 Size: 507 3161 1802 Fragments arranged by size: 3161 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2736 2917 Size: 54 1425 50 275 18 564 342 181 Cuts at: 2917 3806 5435 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 564 342 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2394 2917 1537 Size: 275 582 523 4090 Fragments arranged by size: 4090 582 523 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3400 1353 Size: 2047 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2301 4057 4431 4577 387 Size: 1064 138 712 1756 374 146 1280 Fragments arranged by size: 1756 1280 1064 712 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 3533 4130 4917 5185 1239 Size: 393 630 1271 597 787 268 1524 Fragments arranged by size: 1524 1271 787 630 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2190 2190 Size: 5470 EcoRI G'AATT_C Cuts at: 2431 2779 3157 2431 Size: 348 378 4744 Fragments arranged by size: 4744 378 348 FspI TGC'GCA Cuts at: 3976 3976 Size: 5470 HaeII r_GCGC'y Cuts at: 1678 2106 2222 2309 2321 2334 2551 2593 4617 Size: 428 116 87 12 13 217 42 2024 Cuts at: 4617 1678 Size: 2531 Fragments arranged by size: 2531 2024 428 217 116 87 42 13 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4111 4143 4185 4217 1373 Size: 32 135 32 2539 32 42 32 2626 Fragments arranged by size: 2626 2539 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2814 3060 669 Size: 988 281 876 246 3079 Fragments arranged by size: 3079 988 876 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5470 HpaI GTT'AAC Cuts at: 3060 3060 Size: 5470 KasI G'GCGC_C Cuts at: 2102 2305 2317 2330 2547 2102 Size: 203 12 13 217 5025 Fragments arranged by size: 5025 217 203 13 12 KpnI G_GTAC'C Cuts at: 2795 2795 Size: 5470 MfeI C'AATT_G Cuts at: 3069 3069 Size: 5470 MluI A'CGCG_T Cuts at: 5346 5346 Size: 5470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2534 2565 4584 4768 1305 Size: 1071 158 31 2019 184 2007 Fragments arranged by size: 2019 2007 1071 184 158 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 10 Size: 54 1425 343 906 2742 Fragments arranged by size: 2742 1425 906 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3428 3787 3946 518 Size: 648 843 1419 359 159 2042 Fragments arranged by size: 2042 1419 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2199 2886 3563 4711 4956 1606 Size: 593 687 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 593 245 NaeI GCC'GGC Cuts at: 2316 2394 2316 Size: 78 5392 NarI GG'CG_CC Cuts at: 2103 2306 2318 2331 2548 2103 Size: 203 12 13 217 5025 Fragments arranged by size: 5025 217 203 13 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3979 Fragments arranged by size: 3979 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5470 NgoMIV G'CCGG_C Cuts at: 2314 2392 2314 Size: 78 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5470 NotI GC'GGCC_GC Cuts at: 2917 2917 Size: 5470 NspI r_CATG'y Cuts at: 1132 1229 2828 4373 1132 Size: 97 1599 1545 2229 Fragments arranged by size: 2229 1599 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5470 PciI A'CATG_T Cuts at: 4369 4369 Size: 5470 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5470 PfoI T'CCnGG_A Cuts at: 2278 2278 Size: 5470 PmeI GTTT'AAAC Cuts at: 2774 2774 Size: 5470 PmlI CAC'GTG Cuts at: 2428 2428 Size: 5470 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3539 3571 5052 5084 3539 Size: 32 1481 32 3925 Fragments arranged by size: 3925 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5470 PsiI TTA'TAA Cuts at: 3040 3040 Size: 5470 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2432 2464 2432 Size: 32 5438 PstI C_TGCA'G Cuts at: 830 2822 830 Size: 1992 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5470 SalI G'TCGA_C Cuts at: 1936 2812 1936 Size: 876 4594 SbfI CC_TGCA'GG Cuts at: 2822 2822 Size: 5470 ScaI AGT'ACT Cuts at: 3718 5430 3718 Size: 1712 3758 SfcI C'TryA_G Cuts at: 826 1045 2341 2818 3953 4634 4825 826 Size: 219 1296 477 1135 681 191 1471 Fragments arranged by size: 1471 1296 1135 681 477 219 191 SfoI GGC'GCC Cuts at: 2104 2307 2319 2332 2549 2104 Size: 203 12 13 217 5025 Fragments arranged by size: 5025 217 203 13 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 SgrAI Cr'CCGG_yG Cuts at: 1700 2314 2453 1700 Size: 614 139 4717 Fragments arranged by size: 4717 614 139 SmaI CCC'GGG Cuts at: 1872 2151 2206 2336 2374 2797 1872 Size: 279 55 130 38 423 4545 Fragments arranged by size: 4545 423 279 130 55 38 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3574 4475 4737 820 Size: 197 926 441 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 441 262 197 SnaBI TAC'GTA Cuts at: 493 3261 493 Size: 2768 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5470 SphI G_CATG'C Cuts at: 1132 2828 1132 Size: 1696 3774 SrfI GCCC'GGGC Cuts at: 2336 2336 Size: 5470 SspI AAT'ATT Cuts at: 5 52 3394 5 Size: 47 3342 2081 Fragments arranged by size: 3342 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5470 StyI C'CwwG_G Cuts at: 513 1065 2004 2089 2909 513 Size: 552 939 85 820 3074 Fragments arranged by size: 3074 939 820 552 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3497 3514 3667 3852 4297 325 Size: 1255 1917 17 153 185 445 1498 Fragments arranged by size: 1917 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3716 5428 96 Size: 274 80 33 51 1443 1739 1712 138 Fragments arranged by size: 1739 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5430 5462 5430 Size: 32 5438 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5470 XbaI T'CTAG_A Cuts at: 2806 2806 Size: 5470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5470 XmaI C'CCGG_G Cuts at: 1870 2149 2204 2334 2372 2795 1870 Size: 279 55 130 38 423 4545 Fragments arranged by size: 4545 423 279 130 55 38 XmnI GAAnn'nnTTC Cuts at: 3599 3599 Size: 5470 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3278 276 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI