(Circular) (Six-base) MAPSORT of: pFN21AB8911.seq Check: 3571 from: 1 to: 5455 pFN21AB8911 5455 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2235 2798 1937 Size: 103 195 563 4594 Fragments arranged by size: 4594 563 195 103 Acc65I G'GTAC_C Cuts at: 2283 2776 2283 Size: 493 4962 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AfeI AGC'GCT Cuts at: 2570 2570 Size: 5455 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 2211 4354 5331 1203 Size: 1008 2143 977 1327 Fragments arranged by size: 2143 1327 1008 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2061 1166 Size: 895 4560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2603 2635 2603 Size: 32 5423 AlwNI CAG_nnn'CTG Cuts at: 2596 3198 4770 2596 Size: 602 1572 3281 Fragments arranged by size: 3281 1572 602 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2481 2764 2990 3142 5251 1493 Size: 988 283 226 152 2109 1697 Fragments arranged by size: 2109 1697 988 283 226 152 AscI GG'CGCG_CC Cuts at: 2373 2373 Size: 5455 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2329 2405 2780 1870 Size: 73 386 76 375 4545 Fragments arranged by size: 4545 386 375 76 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2194 2283 2308 2776 Size: 325 217 627 396 11 89 25 468 Cuts at: 2776 4231 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 468 396 325 217 89 25 11 BanII G_rGCy'C Cuts at: 1391 2111 2207 2774 1391 Size: 720 96 567 4072 Fragments arranged by size: 4072 720 567 96 BbeI G_GCGC'C Cuts at: 2187 2198 2312 2187 Size: 11 114 5330 Fragments arranged by size: 5330 114 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 4066 136 Size: 107 122 71 631 605 520 1874 1525 Fragments arranged by size: 1874 1525 631 605 520 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2024 2201 2872 2024 Size: 177 671 4607 Fragments arranged by size: 4607 671 177 Bme1580I G_kGCm'C Cuts at: 1790 2402 3516 4672 1790 Size: 612 1114 1156 2573 Fragments arranged by size: 2573 1156 1114 612 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4144 442 Size: 416 53 83 399 24 1769 958 1753 Fragments arranged by size: 1769 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2159 2321 2618 4115 1239 Size: 822 98 162 297 1497 2579 Fragments arranged by size: 2579 1497 822 297 162 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3580 4445 4743 3580 Size: 865 298 4292 Fragments arranged by size: 4292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4118 882 Size: 617 2619 2219 Fragments arranged by size: 2619 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2195 2262 2309 Size: 53 83 186 936 651 11 67 47 Cuts at: 2309 3262 3644 4279 275 Size: 953 382 635 1451 Fragments arranged by size: 1451 953 936 651 635 382 186 83 67 53 47 11 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2603 2633 1178 Size: 30 165 30 1200 30 4000 Fragments arranged by size: 4000 1200 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2657 1186 Size: 1471 3984 BseYI C'CCAG_C Cuts at: 2368 4658 5154 2368 Size: 2290 496 2669 Fragments arranged by size: 2669 2290 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2173 2528 1163 Size: 573 437 355 4090 Fragments arranged by size: 4090 573 437 355 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2274 2905 3666 4694 1540 Size: 275 186 273 631 761 1028 2301 Fragments arranged by size: 2301 1028 761 631 275 273 186 BsiHKAI G_wGCw'C Cuts at: 2111 2136 3516 3601 4672 2111 Size: 25 1380 85 1071 2894 Fragments arranged by size: 2894 1380 1071 85 25 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2136 2207 2402 2774 3516 3601 Size: 399 321 25 71 195 372 742 85 Cuts at: 3601 4672 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 321 195 85 71 25 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2810 844 Size: 1427 539 3489 Fragments arranged by size: 3489 1427 539 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3950 4124 65 Size: 1183 2702 174 1396 Fragments arranged by size: 2702 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2173 2269 2305 2709 4099 4286 1700 Size: 110 363 96 36 404 1390 187 2869 Fragments arranged by size: 2869 1390 404 363 187 110 96 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 2373 1925 Size: 448 5007 BssSI C'ACGA_G Cuts at: 2057 2149 3515 4527 2057 Size: 92 1366 1012 2985 Fragments arranged by size: 2985 1366 1012 92 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450 Size: 813 367 385 17 1452 86 12 346 Cuts at: 5450 1972 Size: 1977 Fragments arranged by size: 1977 1452 813 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4903 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3765 3785 2971 Size: 794 20 4641 Fragments arranged by size: 4641 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DraIII CAC_nnn'GTG Cuts at: 2656 2656 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2271 2658 Size: 54 1425 50 275 18 265 176 387 Cuts at: 2658 2902 3791 5420 8 Size: 244 889 1629 43 Fragments arranged by size: 1629 1425 889 387 275 265 244 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2271 2902 1537 Size: 275 459 631 4090 Fragments arranged by size: 4090 631 459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2560 3385 1353 Size: 1207 825 3423 Fragments arranged by size: 3423 1207 825 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 2521 2728 4042 4416 4562 Size: 1064 138 783 149 207 1314 374 146 Cuts at: 4562 387 Size: 1280 Fragments arranged by size: 1314 1280 1064 783 374 207 149 146 138 EcoICRI GAG'CTC Cuts at: 2109 2109 Size: 5455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2159 2321 2618 3518 4115 4902 Size: 393 429 98 162 297 900 597 787 Cuts at: 4902 5170 1239 Size: 268 1524 Fragments arranged by size: 1524 900 787 597 429 393 297 268 162 98 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2174 2187 2198 2312 2572 4602 1678 Size: 496 13 11 114 260 2030 2531 Fragments arranged by size: 2531 2030 496 260 114 13 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 2500 2532 4096 Size: 32 135 32 466 32 430 32 1564 Cuts at: 4096 4128 4170 4202 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1564 466 430 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2799 3045 669 Size: 988 281 103 758 246 3079 Fragments arranged by size: 3079 988 758 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KasI G'GCGC_C Cuts at: 2183 2194 2308 2183 Size: 11 114 5330 Fragments arranged by size: 5330 114 11 KpnI G_GTAC'C Cuts at: 2287 2780 2287 Size: 493 4962 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 2211 5331 2211 Size: 3120 2335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2097 2660 10 Size: 54 1425 343 265 563 2805 Fragments arranged by size: 2805 1425 563 343 265 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 3413 3772 3931 518 Size: 648 895 1352 359 159 2042 Fragments arranged by size: 2042 1352 895 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2365 2871 3548 4696 4941 1606 Size: 594 165 506 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 506 245 165 NaeI GCC'GGC Cuts at: 2271 2307 2271 Size: 36 5419 NarI GG'CG_CC Cuts at: 2184 2195 2309 2184 Size: 11 114 5330 Fragments arranged by size: 5330 114 11 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4903 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NgoMIV G'CCGG_C Cuts at: 2269 2305 2269 Size: 36 5419 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2813 4358 1132 Size: 97 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 97 PasI CC'CwG_GG Cuts at: 1348 2257 1348 Size: 909 4546 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 1108 Size: 1488 3967 PfoI T'CCnGG_A Cuts at: 2216 2558 2216 Size: 342 5113 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2603 2635 3524 3556 5037 5069 2603 Size: 32 889 32 1481 32 2989 Fragments arranged by size: 2989 1481 889 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 SacI G_AGCT'C Cuts at: 2111 2111 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2039 2797 1936 Size: 103 758 4594 Fragments arranged by size: 4594 758 103 SapI GCTCTTCn'nnn_ Cuts at: 2560 2560 Size: 5455 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2803 3938 4619 4810 826 Size: 219 1758 1135 681 191 1471 Fragments arranged by size: 1758 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2185 2196 2310 2185 Size: 11 114 5330 Fragments arranged by size: 5330 114 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 2173 1700 Size: 473 4982 SmaI CCC'GGG Cuts at: 1872 2331 2782 1872 Size: 459 451 4545 Fragments arranged by size: 4545 459 451 SmlI C'TyrA_G Cuts at: 820 1017 1943 3559 4460 4722 820 Size: 197 926 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2894 513 Size: 552 1829 3074 Fragments arranged by size: 3074 1829 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2409 3701 5413 Size: 274 80 33 51 1443 432 1292 1712 Cuts at: 5413 96 Size: 138 Fragments arranged by size: 1712 1443 1292 432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2329 2780 1870 Size: 459 451 4545 Fragments arranged by size: 4545 459 451 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI