(Circular) (Six-base) MAPSORT of: pFN21AB8908.seq Check: 8687 from: 1 to: 5431 pFN21AB8908 5431 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3494 4878 5146 1632 Size: 1862 1384 268 1917 Fragments arranged by size: 1917 1862 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 3174 4746 3174 Size: 1572 3859 ApaI G_GGCC'C Cuts at: 2130 2130 Size: 5431 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2264 2740 2966 3118 5227 1493 Size: 771 476 226 152 2109 1697 Fragments arranged by size: 2109 1697 771 476 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2652 2756 1870 Size: 73 709 104 4545 Fragments arranged by size: 4545 709 104 73 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 2192 2752 4207 618 Size: 325 217 627 353 52 560 1455 1842 Fragments arranged by size: 1842 1455 627 560 353 325 217 52 BanII G_rGCy'C Cuts at: 1391 2012 2130 2311 2653 2750 1391 Size: 621 118 181 342 97 4072 Fragments arranged by size: 4072 621 342 181 118 97 BbeI G_GCGC'C Cuts at: 2144 2144 Size: 5431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2311 2345 3622 3656 1903 Size: 34 374 34 1277 34 3678 Fragments arranged by size: 3678 1277 374 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2586 5426 2586 Size: 2840 2591 BlpI GC'TnA_GC Cuts at: 2240 2310 2848 2240 Size: 70 538 4823 Fragments arranged by size: 4823 538 70 Bme1580I G_kGCm'C Cuts at: 1790 2130 3492 4648 1790 Size: 340 1362 1156 2573 Fragments arranged by size: 2573 1362 1156 340 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 4120 442 Size: 416 53 83 399 24 1745 958 1753 Fragments arranged by size: 1753 1745 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4091 1239 Size: 2852 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4421 4719 3556 Size: 865 298 4268 Fragments arranged by size: 4268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2425 4094 882 Size: 617 926 1669 2219 Fragments arranged by size: 2219 1669 926 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2256 3238 3620 Size: 53 83 186 936 608 115 982 382 Cuts at: 3620 4255 275 Size: 635 1451 Fragments arranged by size: 1451 982 936 635 608 382 186 115 83 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2440 2470 1178 Size: 30 165 30 777 30 230 30 4139 Fragments arranged by size: 4139 777 230 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2675 2708 2720 1186 Size: 1489 33 12 3897 Fragments arranged by size: 3897 1489 33 12 BseYI C'CCAG_C Cuts at: 2408 4634 5130 2408 Size: 2226 496 2709 Fragments arranged by size: 2709 2226 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4858 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2237 3492 3577 4648 2012 Size: 225 1255 85 1071 2795 Fragments arranged by size: 2795 1255 1071 225 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 BsmBI CGTCTCn'nnnn_ Cuts at: 2247 2409 2247 Size: 162 5269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2130 2237 2311 2653 2750 3492 Size: 399 222 118 107 74 342 97 742 Cuts at: 3492 3577 4648 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 222 118 107 97 85 74 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 844 Size: 1942 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2065 2082 2475 4075 4262 1700 Size: 110 238 17 17 393 1600 187 2869 Fragments arranged by size: 2869 1600 393 238 187 110 17 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2586 2761 3128 3513 3530 4982 5068 5080 Size: 614 175 367 385 17 1452 86 12 Cuts at: 5080 5426 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 614 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2188 2248 2423 513 Size: 552 1123 60 175 3521 Fragments arranged by size: 3521 1123 552 175 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2461 2947 3741 3761 2461 Size: 486 794 20 4131 Fragments arranged by size: 4131 794 486 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396 Size: 54 1425 50 275 18 1048 889 1629 Cuts at: 5396 8 Size: 43 Fragments arranged by size: 1629 1425 1048 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2697 3361 1353 Size: 1332 12 664 3423 Fragments arranged by size: 3423 1332 664 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2344 4018 4392 4538 387 Size: 1064 138 755 1674 374 146 1280 Fragments arranged by size: 1674 1280 1064 755 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5431 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3494 4091 4878 5146 1239 Size: 393 1862 597 787 268 1524 Fragments arranged by size: 1862 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2144 2285 4578 1678 Size: 466 141 2293 2531 Fragments arranged by size: 2531 2293 466 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 4072 4104 4146 Size: 32 135 32 761 32 1707 32 42 Cuts at: 4146 4178 1373 Size: 32 2626 Fragments arranged by size: 2626 1707 761 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2023 2775 3021 669 Size: 988 281 85 752 246 3079 Fragments arranged by size: 3079 988 752 281 246 85 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2140 2140 Size: 5431 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 2519 4545 4729 1305 Size: 927 287 2026 184 2007 Fragments arranged by size: 2026 2007 927 287 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3748 3907 518 Size: 648 2223 359 159 2042 Fragments arranged by size: 2223 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2847 3524 4672 4917 1606 Size: 1241 677 1148 245 2120 Fragments arranged by size: 2120 1241 1148 677 245 NaeI GCC'GGC Cuts at: 2050 2050 Size: 5431 NarI GG'CG_CC Cuts at: 2141 2141 Size: 5431 NcoI C'CATG_G Cuts at: 513 1065 2423 513 Size: 552 1358 3521 Fragments arranged by size: 3521 1358 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NgoMIV G'CCGG_C Cuts at: 2048 2048 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2789 4334 1132 Size: 97 1560 1545 2229 Fragments arranged by size: 2229 1560 1545 97 PasI CC'CwG_GG Cuts at: 1348 2560 1348 Size: 1212 4219 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 2116 1108 Size: 1008 4423 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 2251 3500 3532 5013 5045 2219 Size: 32 1249 32 1481 32 2605 Fragments arranged by size: 2605 1481 1249 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PspOMI G'GGCC_C Cuts at: 2126 2126 Size: 5431 PstI C_TGCA'G Cuts at: 830 2783 830 Size: 1953 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2779 3914 4595 4786 826 Size: 219 1734 1135 681 191 1471 Fragments arranged by size: 1734 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2142 2142 Size: 5431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3535 4436 4698 820 Size: 197 926 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2423 2487 2870 513 Size: 552 1358 64 383 3074 Fragments arranged by size: 3074 1358 552 383 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 3458 3475 3628 3813 4258 325 Size: 1255 1005 873 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1005 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3677 5389 96 Size: 274 80 33 51 1443 1700 1712 138 Fragments arranged by size: 1712 1700 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2440 2472 5391 5423 2180 Size: 32 228 32 2919 32 2188 Fragments arranged by size: 2919 2188 228 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 3560 3560 Size: 5431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI