(Circular) (Six-base) MAPSORT of: pFN21AB8832.seq Check: 1796 from: 1 to: 6061 pFN21AB8832 6061 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2664 2664 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2779 2947 3115 3199 4124 5508 5776 1632 Size: 1147 168 168 84 925 1384 268 1917 Fragments arranged by size: 1917 1384 1147 925 268 168 168 84 AfeI AGC'GCT Cuts at: 2153 2888 2153 Size: 735 5326 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 2559 4960 5937 1203 Size: 1356 2401 977 1327 Fragments arranged by size: 2401 1356 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 2585 4790 2585 Size: 2205 3856 AleI CACnn'nnGTG Cuts at: 1166 2115 2604 2729 2854 3053 1166 Size: 949 489 125 125 199 4174 Fragments arranged by size: 4174 949 489 199 125 125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2371 3804 5376 2371 Size: 1433 1572 3056 Fragments arranged by size: 3056 1572 1433 ApaI G_GGCC'C Cuts at: 3253 3253 Size: 6061 ApaLI G'TGCA_C Cuts at: 2157 2903 4118 5274 2157 Size: 746 1215 1156 2944 Fragments arranged by size: 2944 1215 1156 746 ApoI r'AATT_y Cuts at: 1493 3370 3596 3748 5857 1493 Size: 1877 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2668 2945 3113 3386 1870 Size: 73 725 277 168 273 4545 Fragments arranged by size: 4545 725 277 273 168 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2358 2528 3382 4837 618 Size: 325 217 627 571 170 854 1455 1842 Fragments arranged by size: 1842 1455 854 627 571 325 217 170 BanII G_rGCy'C Cuts at: 1391 2110 2573 2639 3253 3380 1391 Size: 719 463 66 614 127 4072 Fragments arranged by size: 4072 719 614 463 127 66 BbeI G_GCGC'C Cuts at: 2362 2532 2362 Size: 170 5891 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5090 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2860 2894 3292 3326 4252 4286 1903 Size: 34 923 34 398 34 926 34 3678 Fragments arranged by size: 3678 926 923 398 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2250 2380 2838 Size: 107 122 71 631 605 578 130 458 Cuts at: 2838 4672 136 Size: 1834 1525 Fragments arranged by size: 1834 1525 631 605 578 458 130 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2161 2510 2693 2907 2945 3253 3305 4122 Size: 371 349 183 214 38 308 52 817 Cuts at: 4122 5278 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 817 371 349 308 214 183 52 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 4721 1239 Size: 1496 1986 2579 Fragments arranged by size: 2579 1986 1496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 3051 3852 493 Size: 711 1356 491 801 2702 Fragments arranged by size: 2702 1356 801 711 491 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2359 2529 2595 3868 Size: 53 83 186 936 826 170 66 1273 Cuts at: 3868 4250 4885 275 Size: 382 635 1451 Fragments arranged by size: 1451 1273 936 826 635 382 186 170 83 66 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6061 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 3139 3140 1163 Size: 573 335 1068 1 4084 Fragments arranged by size: 4084 1068 573 335 1 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 2254 2320 2956 2971 3511 Size: 275 186 124 129 66 636 15 540 Cuts at: 3511 4272 5300 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 636 540 275 186 129 124 66 15 BsiHKAI G_wGCw'C Cuts at: 2110 2161 2639 2907 4122 4207 5278 2110 Size: 51 478 268 1215 85 1071 2893 Fragments arranged by size: 2893 1215 1071 478 268 85 51 BsiWI C'GTAC_G Cuts at: 2766 2766 Size: 6061 BsmI GAATG_Cn' Cuts at: 2147 3572 3665 2147 Size: 1425 93 4543 Fragments arranged by size: 4543 1425 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2161 2510 2573 2639 2693 2907 Size: 399 320 51 349 63 66 54 214 Cuts at: 2907 2945 3253 3305 3380 4122 4207 5278 1391 Size: 38 308 52 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 320 308 214 85 75 66 63 54 52 51 38 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2664 3416 844 Size: 1820 752 3489 Fragments arranged by size: 3489 1820 752 BsrBI CCG'CTC Cuts at: 1394 1823 2380 2845 2973 3228 3948 1394 Size: 429 557 465 128 255 720 3507 Fragments arranged by size: 3507 720 557 465 429 255 128 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2280 2319 2355 2991 3089 3325 4705 Size: 110 470 39 36 636 98 236 1380 Cuts at: 4705 4892 1700 Size: 187 2869 Fragments arranged by size: 2869 1380 636 470 236 187 110 98 39 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 2424 2499 2549 3259 1925 Size: 499 75 50 710 4727 Fragments arranged by size: 4727 710 499 75 50 BssSI C'ACGA_G Cuts at: 2047 4121 5133 2047 Size: 2074 1012 2975 Fragments arranged by size: 2975 2074 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2165 2226 794 Size: 1371 61 4629 Fragments arranged by size: 4629 1371 61 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2606 2606 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056 Size: 1419 367 385 17 1452 86 12 346 Cuts at: 6056 1972 Size: 1977 Fragments arranged by size: 1977 1452 1419 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5509 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2302 487 Size: 683 56 1076 4246 Fragments arranged by size: 4246 1076 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2227 2510 3152 3303 3577 4371 4391 2031 Size: 196 283 642 151 274 794 20 3701 Fragments arranged by size: 3701 794 642 283 274 196 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2200 2251 Size: 54 1425 50 275 18 292 78 51 Cuts at: 2251 2317 2846 2953 3091 3182 3508 4397 6026 Size: 66 529 107 138 91 326 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1629 1425 889 529 326 292 275 138 107 91 78 66 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2122 2251 2317 2953 3508 1537 Size: 275 310 129 66 636 555 4090 Fragments arranged by size: 4090 636 555 310 275 129 66 EarI CTCTTCn'nnn_ Cuts at: 1353 2438 3991 1353 Size: 1085 1553 3423 Fragments arranged by size: 3423 1553 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2231 4648 5022 5168 387 Size: 1064 138 642 2417 374 146 1280 Fragments arranged by size: 2417 1280 1064 642 374 146 138 EcoICRI GAG'CTC Cuts at: 2108 2637 2108 Size: 529 5532 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2735 2779 2947 3115 3199 4124 4721 Size: 393 1103 44 168 168 84 925 597 Cuts at: 4721 5508 5776 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1103 925 787 597 393 268 168 168 84 44 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 3196 4567 3196 Size: 1371 4690 HaeII r_GCGC'y Cuts at: 1678 2155 2326 2345 2362 2532 2724 2890 5208 Size: 477 171 19 17 170 192 166 2318 Cuts at: 5208 1678 Size: 2531 Fragments arranged by size: 2531 2318 477 192 171 170 166 19 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2571 2603 4702 4734 4776 Size: 32 135 32 999 32 2099 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 2099 999 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 2236 3146 748 Size: 1488 910 3663 Fragments arranged by size: 3663 1488 910 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2358 2528 2358 Size: 170 5891 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2916 5175 5359 1305 Size: 1611 2259 184 2007 Fragments arranged by size: 2259 2007 1611 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2202 10 Size: 54 1425 343 370 3869 Fragments arranged by size: 3869 1425 370 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2115 2558 2604 2729 2854 3053 3106 Size: 648 949 443 46 125 125 199 53 Cuts at: 3106 4019 4378 4537 518 Size: 913 359 159 2042 Fragments arranged by size: 2042 949 913 648 443 359 199 159 125 125 53 46 MspA1I CmG'CkG Cuts at: 1606 2033 2250 2391 2398 2496 3024 3188 3247 Size: 427 217 141 7 98 528 164 59 Cuts at: 3247 3272 3477 4154 5302 5547 1606 Size: 25 205 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 528 427 245 217 205 164 141 98 59 25 7 NaeI GCC'GGC Cuts at: 2321 3091 3327 2321 Size: 770 236 5055 Fragments arranged by size: 5055 770 236 NarI GG'CG_CC Cuts at: 2359 2529 2359 Size: 170 5891 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5509 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2319 3089 3325 2319 Size: 770 236 5055 Fragments arranged by size: 5055 770 236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 2953 3508 2953 Size: 555 5506 NspI r_CATG'y Cuts at: 1132 1229 2198 2316 2775 2855 3419 4964 1132 Size: 97 969 118 459 80 564 1545 2229 Fragments arranged by size: 2229 1545 969 564 459 118 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PfoI T'CCnGG_A Cuts at: 2564 2712 2564 Size: 148 5913 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 2560 3051 2560 Size: 491 5570 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 3249 3249 Size: 6061 PstI C_TGCA'G Cuts at: 830 2222 2661 3413 830 Size: 1392 439 752 3478 Fragments arranged by size: 3478 1392 752 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 SacI G_AGCT'C Cuts at: 2110 2639 2110 Size: 529 5532 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 2438 2438 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SexAI A'CCwGG_T Cuts at: 2895 2895 Size: 6061 SfcI C'TryA_G Cuts at: 826 1045 2077 2218 2657 3409 4544 5225 5416 Size: 219 1032 141 439 752 1135 681 191 Cuts at: 5416 826 Size: 1471 Fragments arranged by size: 1471 1135 1032 752 681 439 219 191 141 SfoI GGC'GCC Cuts at: 2360 2530 2360 Size: 170 5891 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 2355 2991 1700 Size: 655 636 4770 Fragments arranged by size: 4770 655 636 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 2198 3419 1132 Size: 1066 1221 3774 Fragments arranged by size: 3774 1221 1066 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 3128 1517 Size: 1611 4450 StyI C'CwwG_G Cuts at: 513 1065 2457 3500 513 Size: 552 1392 1043 3074 Fragments arranged by size: 3074 1392 1043 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2681 2933 4190 2681 Size: 252 1257 4552 Fragments arranged by size: 4552 1257 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI