Restriction map for
pFN21AB8822
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FspI HaeII
HincII HindII HpaI KasI KspI
MaeI MaeII MamI MfeI MflI
MluI MspA1I MunI MvnI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 483 GGTACC G^GTACC
AccIII 921 TCCGGA T^CCGGA
AcsI 937 GAATTT [GA]^AATT[CT]
AcyI 16 GACGCC G[GA]^CG[CT]C
AgeI 262 ACCGGT A^CCGGT
ApaI 1162 GGGCCC GGGCC^C
ApoI 937 GAATTT [GA]^AATT[CT]
Asp718I 483 GGTACC G^GTACC
AvaI 1043 CCCGGG C^[CT]CG[GA]G
BpmI 872 CTCCAG CTGGAG(16/14)
BsaHI 16 GACGCC G[GA]^CG[CT]C
BsaI 824 GAGACC GGTCTC(1/5)
BseAI 921 TCCGGA T^CCGGA
BsoBI 1043 CCCGGG C^[CT]CG[GA]G
BspEI 921 TCCGGA T^CCGGA
CfoI 258 GCGC GCG^C
DraI 1152 TTTAAA TTT^AAA
DsaI 104 CCGTGG C^C[GA][CT]GG
EarI 914 GAAGAG CTCTTC(1/4)
EcoNI 272 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 523 GGCCGGCC GGCCGG^CC
HhaI 258 GCGC GCG^C
Hin1I 16 GACGCC G[GA]^CG[CT]C
HinP1I 258 GCGC G^CGC
HindIII 376 AAGCTT A^AGCTT
KpnI 483 GGTACC GGTAC^C
Ksp632I 914 GAAGAG CTCTTC(1/4)
MroI 921 TCCGGA T^CCGGA
MslI 540 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 524 GCCGGC GCC^GGC
NdeI 1139 CATATG CA^TATG
NgoMIV 524 GCCGGC G^CCGGC
NspI 1055 ACATGC [GA]CATG^[CT]
PinAI 262 ACCGGT A^CCGGT
PpuMI 744 AGGACCC [GA]G^G[AT]CC[CT]
SacI 917 GAGCTC GAGCT^C
SapI 914 GAAGAGC GCTCTTC(1/4)
SgrAI 261 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1043 CCCGGG CCC^GGG
TaqI 954 TCGA T^CGA
TthHB8I 954 TCGA T^CGA
XmaCI 1043 CCCGGG C^CCGGG
XmaI 1043 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 155 GTAC GT^AC
484 GTAC
BlpI 570 GCTCAGC GC^T[ACGT]AGC
847 GCTAAGC
Bpu1102I 570 GCTCAGC GC^T[ACGT]AGC
847 GCTAAGC
BsaWI 262 ACCGGT [AT]^CCGG[AT]
921 TCCGGA
BsmAI 824 GAGAC GTCTC(1/5)
316 GTCTC
BsmFI 348 GGGAC GGGAC(10/14)
806 GGGAC
BsrDI 363 CATTGC GCAATG(2/0)
110 GCAATG
BsrFI 262 ACCGGT [GA]^CCGG[CT]
524 GCCGGC
CelII 570 GCTCAGC GC^T[ACGT]AGC
847 GCTAAGC
Cfr10I 262 ACCGGT [GA]^CCGG[CT]
524 GCCGGC
DpnII 196 GATC ^GATC
831 GATC
EcoT14I 131 CCTTGG C^C[AT][AT]GG
401 CCAAGG
HgaI 16 GACGC GACGC(5/10)
350 GACGC
MaeIII 94 GTGAC ^GT[ACGT]AC
949 GTGAC
MboI 196 GATC ^GATC
831 GATC
MseI 902 TTAA T^TAA
1153 TTAA
NdeII 196 GATC ^GATC
831 GATC
PflMI 714 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCAGACCCTGG
PstI 276 CTGCAG CTGCA^G
501 CTGCAG
RsaI 155 GTAC GT^AC
484 GTAC
Sau3AI 196 GATC ^GATC
831 GATC
StyI 131 CCTTGG C^C[AT][AT]GG
401 CCAAGG
TfiI 161 GATTC G^A[AT]TC
925 GATTC
Tru9I 902 TTAA T^TAA
1153 TTAA
Tsp45I 94 GTGAC ^GT[GC]AC
949 GTGAC
Van91I 714 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BalI BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboII
MluNI MnlI MscI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI SfcI TseI
Tsp509I TspRI XcmI