(Circular) (Six-base) MAPSORT of: pFN21AB8820.seq Check: 7086 from: 1 to: 5857 pFN21AB8820 5857 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 3920 5304 5572 1632 Size: 1361 927 1384 268 1917 Fragments arranged by size: 1917 1384 1361 927 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2971 3600 5172 2971 Size: 629 1572 3656 Fragments arranged by size: 3656 1572 629 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2475 3182 1870 Size: 73 532 707 4545 Fragments arranged by size: 4545 707 532 73 AvrII C'CTAG_G Cuts at: 2653 2653 Size: 5857 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3003 3010 3178 4633 618 Size: 325 217 627 1216 7 168 1455 1842 Fragments arranged by size: 1842 1455 1216 627 325 217 168 7 BanII G_rGCy'C Cuts at: 1391 3176 1391 Size: 1785 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 2149 2329 928 Size: 971 238 12 180 4456 Fragments arranged by size: 4456 971 238 180 12 BbvCI CC'TCA_GC Cuts at: 2186 2468 2723 2186 Size: 282 255 5320 Fragments arranged by size: 5320 282 255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2236 4468 136 Size: 107 122 71 631 605 564 2232 1525 Fragments arranged by size: 2232 1525 631 605 564 122 107 71 BglII A'GATC_T Cuts at: 2250 5852 2250 Size: 3602 2255 BlpI GC'TnA_GC Cuts at: 2451 3274 2451 Size: 823 5034 Bme1580I G_kGCm'C Cuts at: 1790 3918 5074 1790 Size: 2128 1156 2573 Fragments arranged by size: 2573 2128 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2147 3588 4546 Size: 416 53 83 399 24 730 1441 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1441 958 730 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2870 2932 4517 1239 Size: 1217 414 62 1585 2579 Fragments arranged by size: 2579 1585 1217 414 62 Bpu10I CC'TnA_GC Cuts at: 2186 2468 2723 2186 Size: 282 255 5320 Fragments arranged by size: 5320 282 255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2227 3648 493 Size: 711 1023 1421 2702 Fragments arranged by size: 2702 1421 1023 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2005 2035 1178 Size: 30 165 30 602 30 5000 Fragments arranged by size: 5000 602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5857 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2369 3918 4003 5074 2369 Size: 1549 85 1071 3152 Fragments arranged by size: 3152 1549 1071 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 BsmBI CGTCTCn'nnnn_ Cuts at: 2533 2533 Size: 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 3176 3918 4003 5074 1391 Size: 399 579 807 742 85 1071 2174 Fragments arranged by size: 2174 1071 807 742 579 399 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2844 3212 844 Size: 2000 368 3489 Fragments arranged by size: 3489 2000 368 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2551 96 Size: 2455 3402 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2250 3187 3554 3939 3956 5408 5494 5506 Size: 278 937 367 385 17 1452 86 12 Cuts at: 5506 5852 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 937 385 367 346 278 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2319 513 Size: 552 939 315 4051 Fragments arranged by size: 4051 939 552 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2887 487 Size: 683 56 1661 3457 Fragments arranged by size: 3457 1661 683 56 BtsI GCAGTG_nn' Cuts at: 2013 2130 2297 2914 3018 3373 4167 4187 2013 Size: 117 167 617 104 355 794 20 3683 Fragments arranged by size: 3683 794 617 355 167 117 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 2751 3161 2751 Size: 410 5447 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2268 2523 Size: 54 1425 50 275 18 177 261 255 Cuts at: 2523 3304 4193 5822 8 Size: 781 889 1629 43 Fragments arranged by size: 1629 1425 889 781 275 261 255 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2633 3787 1353 Size: 1280 1154 3423 Fragments arranged by size: 3423 1280 1154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2254 2951 4444 4818 4964 387 Size: 1064 138 665 697 1493 374 146 1280 Fragments arranged by size: 1493 1280 1064 697 665 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2870 2932 2993 3920 4517 5304 Size: 393 824 414 62 61 927 597 787 Cuts at: 5304 5572 1239 Size: 268 1524 Fragments arranged by size: 1524 927 824 787 597 414 393 268 62 61 EcoNI CCTnn'n_nnAGG Cuts at: 2461 2461 Size: 5857 EcoO109I rG'GnC_Cy Cuts at: 2472 2472 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 5004 1678 Size: 3326 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 4498 4530 4572 Size: 32 135 32 1523 32 1371 32 42 Cuts at: 4572 4604 1373 Size: 32 2626 Fragments arranged by size: 2626 1523 1371 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2811 4971 5155 1305 Size: 996 510 2160 184 2007 Fragments arranged by size: 2160 2007 996 510 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 3597 Fragments arranged by size: 3597 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 3815 4174 4333 518 Size: 648 1152 1497 359 159 2042 Fragments arranged by size: 2042 1497 1152 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2190 3042 3273 3950 5098 5343 1606 Size: 584 852 231 677 1148 245 2120 Fragments arranged by size: 2120 1148 852 677 584 245 231 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2508 1108 Size: 1400 4457 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2472 2472 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PstI C_TGCA'G Cuts at: 830 2114 2764 3209 830 Size: 1284 650 445 3478 Fragments arranged by size: 3478 1284 650 445 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 3042 3042 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SapI GCTCTTCn'nnn_ Cuts at: 2633 2633 Size: 5857 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2110 2161 2760 3205 4340 5021 5212 Size: 219 1065 51 599 445 1135 681 191 Cuts at: 5212 826 Size: 1471 Fragments arranged by size: 1471 1135 1065 681 599 445 219 191 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2477 3184 1872 Size: 605 707 4545 Fragments arranged by size: 4545 707 605 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 3029 1517 Size: 1512 4345 StyI C'CwwG_G Cuts at: 513 1065 2004 2571 2653 2766 3097 3296 513 Size: 552 939 567 82 113 331 199 3074 Fragments arranged by size: 3074 939 567 552 331 199 113 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2551 4103 5815 Size: 274 80 33 51 1443 574 1552 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1552 1443 574 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2005 2037 5817 5849 2005 Size: 32 3780 32 2013 Fragments arranged by size: 3780 2013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 2872 3193 2872 Size: 321 5536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2539 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2475 3182 1870 Size: 605 707 4545 Fragments arranged by size: 4545 707 605 XmnI GAAnn'nnTTC Cuts at: 2627 2978 3986 2627 Size: 351 1008 4498 Fragments arranged by size: 4498 1008 351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI