(Circular) (Six-base) MAPSORT of: pFN21AB8819.seq Check: 3598 from: 1 to: 5851 pFN21AB8819 5851 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3135 3135 Size: 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 2978 3914 5298 5566 1632 Size: 1037 309 936 1384 268 1917 Fragments arranged by size: 1917 1384 1037 936 309 268 AflII C'TTAA_G Cuts at: 820 1017 2302 820 Size: 197 1285 4369 Fragments arranged by size: 4369 1285 197 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 2387 Size: 32 5819 AlwNI CAG_nnn'CTG Cuts at: 3594 5166 3594 Size: 1572 4279 ApaI G_GGCC'C Cuts at: 2368 2368 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 2242 2546 2966 3160 3386 3538 5647 1493 Size: 749 304 420 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 749 420 304 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 2256 4405 160 Size: 872 1224 2149 1606 Fragments arranged by size: 2149 1606 1224 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 3176 1870 Size: 73 1233 4545 Fragments arranged by size: 4545 1233 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2908 3172 4627 618 Size: 325 217 627 1121 264 1455 1842 Fragments arranged by size: 1842 1455 1121 627 325 264 217 BanII G_rGCy'C Cuts at: 1391 2368 3107 3170 1391 Size: 977 739 63 4072 Fragments arranged by size: 4072 977 739 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BbvCI CC'TCA_GC Cuts at: 2568 2876 2568 Size: 308 5543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2181 2765 4462 Size: 107 122 71 631 605 509 584 1697 Cuts at: 4462 136 Size: 1525 Fragments arranged by size: 1697 1525 631 605 584 509 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 2368 3912 5068 1790 Size: 578 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 578 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2528 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 4511 1239 Size: 1095 2177 2579 Fragments arranged by size: 2579 2177 1095 Bpu10I CC'TnA_GC Cuts at: 2568 2876 2568 Size: 308 5543 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 3976 4841 5139 2036 Size: 1940 865 298 2748 Fragments arranged by size: 2748 1940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2289 4514 882 Size: 617 790 2225 2219 Fragments arranged by size: 2225 2219 790 617 BsaAI yAC'GTr Cuts at: 493 1204 2068 3642 493 Size: 711 864 1574 2702 Fragments arranged by size: 2702 1574 864 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2161 4284 4682 4956 5103 5514 2161 Size: 2123 398 274 147 411 2498 Fragments arranged by size: 2498 2123 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5626 Fragments arranged by size: 5626 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5851 BseYI C'CCAG_C Cuts at: 5054 5550 5054 Size: 496 5355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2827 3912 3997 5068 2827 Size: 1085 85 1071 3610 Fragments arranged by size: 3610 1085 1071 85 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 BsmBI CGTCTCn'nnnn_ Cuts at: 2619 2619 Size: 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 2827 3107 3170 3912 3997 5068 Size: 399 578 459 280 63 742 85 1071 Cuts at: 5068 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 578 459 399 280 85 63 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3135 3206 844 Size: 2291 71 3489 Fragments arranged by size: 3489 2291 71 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2060 2560 3738 1394 Size: 429 190 47 500 1178 3507 Fragments arranged by size: 3507 1178 500 429 190 47 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 2750 3911 4923 2750 Size: 1161 1012 3678 Fragments arranged by size: 3678 1161 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 2164 1184 Size: 980 4871 BstXI CCAn_nnnn'nTGG Cuts at: 3076 3076 Size: 5851 BstYI r'GATC_y Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846 Size: 1209 367 385 17 1452 86 12 346 Cuts at: 5846 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2211 513 Size: 552 939 207 4153 Fragments arranged by size: 4153 939 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 487 Size: 683 56 1210 3902 Fragments arranged by size: 3902 1210 683 56 BtsI GCAGTG_nn' Cuts at: 3081 3367 4161 4181 3081 Size: 286 794 20 4751 Fragments arranged by size: 4751 794 286 20 ClaI AT'CG_AT Cuts at: 1554 2796 1554 Size: 1242 4609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2087 4858 809 Size: 507 771 2771 1802 Fragments arranged by size: 2771 1802 771 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 3091 3298 Size: 54 1425 50 275 18 639 622 207 Cuts at: 3298 4187 5816 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 639 622 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3054 3781 1353 Size: 1197 504 727 3423 Fragments arranged by size: 3423 1197 727 504 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 2875 4438 4812 4958 387 Size: 1064 138 672 614 1563 374 146 1280 Fragments arranged by size: 1563 1280 1064 672 614 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2669 2978 3914 4511 5298 5566 Size: 393 702 335 309 936 597 787 268 Cuts at: 5566 1239 Size: 1524 Fragments arranged by size: 1524 936 787 702 597 393 335 309 268 EcoNI CCTnn'n_nnAGG Cuts at: 3131 3131 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2364 2365 2364 Size: 1 5850 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 4998 1678 Size: 3320 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 4492 4524 4566 Size: 32 135 32 845 32 2043 32 42 Cuts at: 4566 4598 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 845 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 3195 3441 669 Size: 988 281 259 998 246 3079 Fragments arranged by size: 3079 998 988 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 2197 3441 2197 Size: 1244 4607 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4965 5149 1305 Size: 3660 184 2007 Fragments arranged by size: 3660 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 3809 4168 4327 518 Size: 648 1333 1310 359 159 2042 Fragments arranged by size: 2042 1333 1310 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2213 2474 3267 3944 5092 5337 1606 Size: 607 261 793 677 1148 245 2120 Fragments arranged by size: 2120 1148 793 677 607 261 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4360 Fragments arranged by size: 4360 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2104 3122 3209 4754 1132 Size: 97 875 1018 87 1545 2229 Fragments arranged by size: 2229 1545 1018 875 97 87 PasI CC'CwG_GG Cuts at: 1348 2179 2903 3127 1348 Size: 831 724 224 4072 Fragments arranged by size: 4072 831 724 224 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2364 2364 Size: 5851 PstI C_TGCA'G Cuts at: 830 3203 830 Size: 2373 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 SacII CC_GC'GG Cuts at: 2214 2214 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SapI GCTCTTCn'nnn_ Cuts at: 2550 2550 Size: 5851 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 3199 4334 5015 5206 826 Size: 219 2154 1135 681 191 1471 Fragments arranged by size: 2154 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2051 2302 3955 4856 5118 820 Size: 197 926 108 251 1653 901 262 1553 Fragments arranged by size: 1653 1553 926 901 262 251 197 108 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 2122 1517 Size: 605 5246 StyI C'CwwG_G Cuts at: 513 1065 2004 3290 513 Size: 552 939 1286 3074 Fragments arranged by size: 3074 1286 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3878 3895 4048 4233 4678 325 Size: 1255 480 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 3021 3053 5811 5843 2207 Size: 32 782 32 2758 32 2215 Fragments arranged by size: 2758 2215 782 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2531 3004 2531 Size: 473 5378 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI