(Circular) (Six-base) MAPSORT of: pFN21AB8817.seq Check: 5473 from: 1 to: 5848 pFN21AB8817 5848 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2314 2463 3191 1937 Size: 377 149 728 4594 Fragments arranged by size: 4594 728 377 149 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 3911 5295 5563 1632 Size: 1292 987 1384 268 1917 Fragments arranged by size: 1917 1384 1292 987 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 2027 2398 2896 3591 5163 2027 Size: 371 498 695 1572 2712 Fragments arranged by size: 2712 1572 695 498 371 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2234 3157 3383 3535 5644 1493 Size: 741 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 923 741 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2380 3169 4624 618 Size: 325 217 627 593 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 593 325 217 BanII G_rGCy'C Cuts at: 1391 3167 1391 Size: 1776 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2422 3740 4950 2422 Size: 1318 1210 3320 Fragments arranged by size: 3320 1318 1210 BclI T'GATC_A Cuts at: 1548 2229 1548 Size: 681 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2279 2540 4459 Size: 107 122 71 631 605 607 261 1919 Cuts at: 4459 136 Size: 1525 Fragments arranged by size: 1919 1525 631 607 605 261 122 107 71 BglII A'GATC_T Cuts at: 2290 2481 5843 2290 Size: 191 3362 2295 Fragments arranged by size: 3362 2295 191 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2385 2536 3909 5065 1790 Size: 595 151 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 595 151 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2781 3113 4508 1239 Size: 1542 332 1395 2579 Fragments arranged by size: 2579 1542 1395 332 Bpu10I CC'TnA_GC Cuts at: 2112 2112 Size: 5848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 2582 5842 2582 Size: 3260 2588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 1186 Size: 1462 4386 BseYI C'CCAG_C Cuts at: 5051 5547 5051 Size: 496 5352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 1163 Size: 573 304 4971 Fragments arranged by size: 4971 573 304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2954 3909 3994 5065 2954 Size: 955 85 1071 3737 Fragments arranged by size: 3737 1071 955 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2536 2954 3167 3909 3994 5065 Size: 399 595 151 418 213 742 85 1071 Cuts at: 5065 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 595 418 399 213 151 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2268 4343 4517 65 Size: 1183 1020 2075 174 1396 Fragments arranged by size: 2075 1396 1183 1020 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 4492 4679 1700 Size: 110 635 2047 187 2869 Fragments arranged by size: 2869 2047 635 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2812 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2290 2481 3178 3545 3930 3947 5399 5485 Size: 318 191 697 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 697 385 367 346 318 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3364 4158 4178 3364 Size: 794 20 5034 Fragments arranged by size: 5034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DraIII CAC_nnn'GTG Cuts at: 2310 2380 2310 Size: 70 5778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 4855 809 Size: 507 1575 1964 1802 Fragments arranged by size: 1964 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 3295 4184 Size: 54 1425 50 275 18 723 742 889 Cuts at: 4184 5813 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 742 723 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2633 3778 1353 Size: 1280 1145 3423 Fragments arranged by size: 3423 1280 1145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2781 2924 3113 3911 4508 5295 5563 Size: 393 1149 143 189 798 597 787 268 Cuts at: 5563 1239 Size: 1524 Fragments arranged by size: 1524 1149 798 787 597 393 268 189 143 EcoO109I rG'GnC_Cy Cuts at: 2360 2395 2893 2360 Size: 35 498 5315 Fragments arranged by size: 5315 498 35 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 2249 4354 2249 Size: 2105 3743 FspAI rTGC'GCAy Cuts at: 2249 2249 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 4995 1678 Size: 3317 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 3192 3438 669 Size: 988 281 940 314 246 3079 Fragments arranged by size: 3079 988 940 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3021 4962 5146 1305 Size: 1716 1941 184 2007 Fragments arranged by size: 2007 1941 1716 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 10 Size: 54 1425 343 723 3303 Fragments arranged by size: 3303 1425 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2807 3806 4165 4324 518 Size: 648 1641 999 359 159 2042 Fragments arranged by size: 2042 1641 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2844 3027 3037 3264 3941 5089 5334 1606 Size: 1238 183 10 227 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 677 245 227 183 10 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2248 3206 4751 1132 Size: 97 1019 958 1545 2229 Fragments arranged by size: 2229 1545 1019 958 97 PasI CC'CwG_GG Cuts at: 1348 2392 1348 Size: 1044 4804 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 2044 2398 2896 1108 Size: 936 354 498 4060 Fragments arranged by size: 4060 936 498 354 PfoI T'CCnGG_A Cuts at: 2562 2589 2758 2895 2562 Size: 27 169 137 5515 Fragments arranged by size: 5515 169 137 27 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2360 2893 2360 Size: 533 5315 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2142 2174 2981 3013 2142 Size: 32 807 32 4977 Fragments arranged by size: 4977 807 32 32 PstI C_TGCA'G Cuts at: 830 3200 830 Size: 2370 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2844 2844 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2633 2633 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SexAI A'CCwGG_T Cuts at: 2376 2376 Size: 5848 SfcI C'TryA_G Cuts at: 826 1045 2659 2998 3196 4331 5012 5203 826 Size: 219 1614 339 198 1135 681 191 1471 Fragments arranged by size: 1614 1471 1135 681 339 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3952 4853 5115 820 Size: 197 926 2009 901 262 1553 Fragments arranged by size: 2009 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 2248 3206 1132 Size: 1116 958 3774 Fragments arranged by size: 3774 1116 958 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 3287 513 Size: 552 939 207 1076 3074 Fragments arranged by size: 3074 1076 939 552 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2404 2612 2910 Size: 274 80 33 51 1443 427 208 298 Cuts at: 2910 4094 5806 96 Size: 1184 1712 138 Fragments arranged by size: 1712 1443 1184 427 298 274 208 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2756 2809 2281 Size: 475 53 5320 Fragments arranged by size: 5320 475 53 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI