(Circular) (Six-base) MAPSORT of: pFN21AB8816.seq Check: 4181 from: 1 to: 5842 pFN21AB8816 5842 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 3185 1937 Size: 673 575 4594 Fragments arranged by size: 4594 673 575 Acc65I G'GTAC_C Cuts at: 2229 3163 2229 Size: 934 4908 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 2771 3905 5289 5557 1632 Size: 645 494 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 645 494 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2742 1184 Size: 1558 4284 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3108 3140 3108 Size: 32 5810 AlwNI CAG_nnn'CTG Cuts at: 2794 3585 5157 2794 Size: 791 1572 3479 Fragments arranged by size: 3479 1572 791 ApaI G_GGCC'C Cuts at: 2854 2854 Size: 5842 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 2809 3020 3095 3151 3377 3529 5638 1493 Size: 1316 211 75 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 211 152 75 56 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2301 2526 3167 1870 Size: 73 358 225 641 4545 Fragments arranged by size: 4545 641 358 225 73 BamHI G'GATC_C Cuts at: 2530 3172 3539 2530 Size: 642 367 4833 Fragments arranged by size: 4833 642 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2229 3163 4618 618 Size: 325 217 627 273 169 934 1455 1842 Fragments arranged by size: 1842 1455 934 627 325 273 217 169 BanII G_rGCy'C Cuts at: 1391 2245 2854 2876 3161 1391 Size: 854 609 22 285 4072 Fragments arranged by size: 4072 854 609 285 22 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 5842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4871 BbvCI CC'TCA_GC Cuts at: 2121 2121 Size: 5842 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BfrBI ATG'CAT Cuts at: 2462 2462 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4453 136 Size: 107 122 71 631 605 2781 1525 Fragments arranged by size: 2781 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5837 5837 Size: 5842 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2308 2854 3903 5059 1790 Size: 518 546 1049 1156 2573 Fragments arranged by size: 2573 1156 1049 546 518 BmgBI CAC'GTC Cuts at: 1318 2309 1318 Size: 991 4851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2879 3573 4531 Size: 416 53 83 399 24 1462 694 958 Cuts at: 4531 442 Size: 1753 Fragments arranged by size: 1753 1462 958 694 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4502 1239 Size: 3263 2579 Bpu10I CC'TnA_GC Cuts at: 2121 2846 2121 Size: 725 5117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2349 2593 3967 4832 5130 2349 Size: 244 1374 865 298 3061 Fragments arranged by size: 3061 1374 865 298 244 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 4505 882 Size: 617 1002 2004 2219 Fragments arranged by size: 2219 2004 1002 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 3649 4031 4666 Size: 53 83 186 936 528 1588 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1588 1451 936 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4275 4673 4947 5094 5505 4275 Size: 398 274 147 411 4612 Fragments arranged by size: 4612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5617 Fragments arranged by size: 5617 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5842 BseYI C'CCAG_C Cuts at: 2028 2150 5045 5541 2028 Size: 122 2895 496 2329 Fragments arranged by size: 2895 2329 496 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 4321 Fragments arranged by size: 4321 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3903 3988 5059 3903 Size: 85 1071 4686 Fragments arranged by size: 4686 1071 85 BsmI GAATG_Cn' Cuts at: 2587 3353 3446 2587 Size: 766 93 4983 Fragments arranged by size: 4983 766 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2245 2308 2854 2876 3161 3903 3988 Size: 399 455 63 546 22 285 742 85 Cuts at: 3988 5059 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 546 455 399 285 85 63 22 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 844 Size: 2353 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2212 4337 4511 65 Size: 1183 964 2125 174 1396 Fragments arranged by size: 2125 1396 1183 964 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 2032 1184 Size: 848 4994 BstYI r'GATC_y Cuts at: 1972 2129 2530 3172 3539 3924 3941 5393 5479 Size: 157 401 642 367 385 17 1452 86 Cuts at: 5479 5491 5837 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 401 385 367 346 157 86 17 12 BstZ17I GTA'TAC Cuts at: 2611 2611 Size: 5842 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 3358 4152 4172 3358 Size: 794 20 5028 Fragments arranged by size: 5028 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3015 3146 3015 Size: 131 5711 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3102 3289 4178 Size: 54 1425 50 275 18 1272 187 889 Cuts at: 4178 5807 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1272 889 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3772 1353 Size: 2419 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4429 4803 4949 387 Size: 1064 138 2840 374 146 1280 Fragments arranged by size: 2840 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 2771 3905 4502 5289 5557 1239 Size: 393 645 494 1134 597 787 268 1524 Fragments arranged by size: 1524 1134 787 645 597 494 393 268 EcoO109I rG'GnC_Cy Cuts at: 2850 2850 Size: 5842 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2927 3017 2927 Size: 90 5752 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2064 4989 1678 Size: 386 2925 2531 Fragments arranged by size: 2925 2531 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 4483 4515 4557 Size: 32 135 32 542 32 2337 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 2337 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KasI G'GCGC_C Cuts at: 2060 2060 Size: 5842 KpnI G_GTAC'C Cuts at: 2233 3167 2233 Size: 934 4908 MfeI C'AATT_G Cuts at: 2502 3441 2502 Size: 939 4903 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3070 4956 5140 1305 Size: 1765 1886 184 2007 Fragments arranged by size: 2007 1886 1765 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3104 10 Size: 54 1425 343 1272 2748 Fragments arranged by size: 2748 1425 1272 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2765 3258 3935 5083 5328 1606 Size: 1159 493 677 1148 245 2120 Fragments arranged by size: 2120 1159 1148 677 493 245 NarI GG'CG_CC Cuts at: 2061 2061 Size: 5842 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 NdeI CA'TA_TG Cuts at: 387 3120 387 Size: 2733 3109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NsiI A_TGCA'T Cuts at: 2464 2464 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2725 3200 4745 1132 Size: 97 1496 475 1545 2229 Fragments arranged by size: 2229 1545 1496 475 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PspOMI G'GGCC_C Cuts at: 2850 2850 Size: 5842 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 3190 4325 5006 5197 826 Size: 219 2145 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2062 2062 Size: 5842 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 2303 3169 1872 Size: 431 866 4545 Fragments arranged by size: 4545 866 431 SmlI C'TyrA_G Cuts at: 820 1017 1943 2364 2572 3946 4847 5109 820 Size: 197 926 421 208 1374 901 262 1553 Fragments arranged by size: 1553 1374 926 901 421 262 208 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 2043 1517 Size: 526 5316 StyI C'CwwG_G Cuts at: 513 1065 2004 2237 3281 513 Size: 552 939 233 1044 3074 Fragments arranged by size: 3074 1044 939 552 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2020 2480 3869 3886 4039 4224 4669 Size: 1255 440 460 1389 17 153 185 445 Cuts at: 4669 325 Size: 1498 Fragments arranged by size: 1498 1389 1255 460 445 440 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4088 5800 96 Size: 274 80 33 51 1443 2111 1712 138 Fragments arranged by size: 2111 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5802 5834 5802 Size: 32 5810 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 2301 3167 1870 Size: 431 866 4545 Fragments arranged by size: 4545 866 431 XmnI GAAnn'nnTTC Cuts at: 2907 2984 3971 2907 Size: 77 987 4778 Fragments arranged by size: 4778 987 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI