(Circular) (Six-base) MAPSORT of: pFN21AB8811.seq Check: 3649 from: 1 to: 5791 pFN21AB8811 5791 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 3134 1937 Size: 229 968 4594 Fragments arranged by size: 4594 968 229 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3089 3854 5238 5506 1632 Size: 1457 765 1384 268 1917 Fragments arranged by size: 1917 1457 1384 765 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 2016 4690 5667 1203 Size: 813 2674 977 1327 Fragments arranged by size: 2674 1327 977 813 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 3534 5106 3534 Size: 1572 4219 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2264 3116 1870 Size: 73 321 852 4545 Fragments arranged by size: 4545 852 321 73 BamHI G'GATC_C Cuts at: 2253 3121 3488 2253 Size: 868 367 4556 Fragments arranged by size: 4556 868 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 2455 2879 3112 4567 Size: 325 217 627 657 11 424 233 1455 Cuts at: 4567 618 Size: 1842 Fragments arranged by size: 1842 1455 657 627 424 325 233 217 11 BanII G_rGCy'C Cuts at: 1391 2420 2478 3110 1391 Size: 1029 58 632 4072 Fragments arranged by size: 4072 1029 632 58 BbeI G_GCGC'C Cuts at: 2448 2459 2883 2448 Size: 11 424 5356 Fragments arranged by size: 5356 424 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BbvCI CC'TCA_GC Cuts at: 2180 2684 2180 Size: 504 5287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 4402 136 Size: 107 122 71 631 605 781 1949 1525 Fragments arranged by size: 1949 1525 781 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 3852 5008 1790 Size: 2062 1156 2573 Fragments arranged by size: 2573 2062 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3522 4480 442 Size: 416 53 83 399 24 2105 958 1753 Fragments arranged by size: 2105 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2993 4451 1239 Size: 1754 1458 2579 Fragments arranged by size: 2579 1754 1458 Bpu10I CC'TnA_GC Cuts at: 2180 2684 2180 Size: 504 5287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2478 2969 4454 882 Size: 617 979 491 1485 2219 Fragments arranged by size: 2219 1485 979 617 491 BsaAI yAC'GTr Cuts at: 493 1204 2019 2035 2728 3582 493 Size: 711 815 16 693 854 2702 Fragments arranged by size: 2702 854 815 711 693 16 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2445 2456 2880 Size: 53 83 186 936 630 282 11 424 Cuts at: 2880 3598 3980 4615 275 Size: 718 382 635 1451 Fragments arranged by size: 1451 936 718 635 630 424 382 282 186 83 53 11 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5566 Fragments arranged by size: 5566 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2801 1186 Size: 1615 4176 BseYI C'CCAG_C Cuts at: 2158 2440 3027 4994 5490 2158 Size: 282 587 1967 496 2459 Fragments arranged by size: 2459 1967 587 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2191 2222 2891 3083 1163 Size: 573 455 31 669 192 3871 Fragments arranged by size: 3871 669 573 455 192 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 2478 2733 3852 3937 5008 2420 Size: 58 255 1119 85 1071 3203 Fragments arranged by size: 3203 1119 1071 255 85 58 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 2478 2733 3110 3852 3937 5008 Size: 399 630 58 255 377 742 85 1071 Cuts at: 5008 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 399 377 255 85 58 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2922 3146 844 Size: 1358 720 224 3489 Fragments arranged by size: 3489 1358 720 224 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4435 4622 1700 Size: 110 2625 187 2869 Fragments arranged by size: 2869 2625 187 110 BsrGI T'GTAC_A Cuts at: 96 2199 96 Size: 2103 3688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 2914 794 Size: 2120 3671 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2440 2704 2758 2113 Size: 327 264 54 5146 Fragments arranged by size: 5146 327 264 54 BstYI r'GATC_y Cuts at: 1972 2253 3121 3488 3873 3890 5342 5428 5440 Size: 281 868 367 385 17 1452 86 12 Cuts at: 5440 5786 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 868 385 367 346 281 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2257 2406 2421 2433 2523 2582 Size: 552 939 253 149 15 12 90 59 Cuts at: 2582 513 Size: 3722 Fragments arranged by size: 3722 939 552 253 149 90 59 15 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2170 2302 2677 2904 487 Size: 683 56 944 132 375 227 3374 Fragments arranged by size: 3374 944 683 375 227 132 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 2895 2928 Size: 54 1425 50 275 18 579 486 33 Cuts at: 2928 2952 3033 3238 4127 5756 8 Size: 24 81 205 889 1629 43 Fragments arranged by size: 1629 1425 889 579 486 275 205 81 54 50 43 33 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 2582 3721 1353 Size: 1130 99 1139 3423 Fragments arranged by size: 3423 1139 1130 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2052 2861 3030 4378 4752 4898 Size: 1064 138 463 809 169 1348 374 146 Cuts at: 4898 387 Size: 1280 Fragments arranged by size: 1348 1280 1064 809 463 374 169 146 138 EcoICRI GAG'CTC Cuts at: 2418 2476 2418 Size: 58 5733 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2993 3089 3854 4451 5238 5506 1239 Size: 393 1361 96 765 597 787 268 1524 Fragments arranged by size: 1524 1361 787 765 597 393 268 96 EcoNI CCTnn'n_nnAGG Cuts at: 2992 2992 Size: 5791 EcoO109I rG'GnC_Cy Cuts at: 2268 2902 2268 Size: 634 5157 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2448 2459 2591 2653 2751 2883 4938 1678 Size: 770 11 132 62 98 132 2055 2531 Fragments arranged by size: 2531 2055 770 132 132 98 62 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373 Size: 32 135 32 2860 32 42 32 2626 Fragments arranged by size: 2860 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3135 3381 669 Size: 988 281 934 263 246 3079 Fragments arranged by size: 3079 988 934 281 263 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KasI G'GCGC_C Cuts at: 2444 2455 2879 2444 Size: 11 424 5356 Fragments arranged by size: 5356 424 11 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2897 2930 2954 10 Size: 54 1425 343 1065 33 24 2847 Fragments arranged by size: 2847 1425 1065 343 54 33 24 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2339 3749 4108 4267 518 Size: 648 924 249 1410 359 159 2042 Fragments arranged by size: 2042 1410 924 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2218 3027 3067 3207 3884 5032 5277 1606 Size: 612 809 40 140 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 612 245 140 40 NarI GG'CG_CC Cuts at: 2445 2456 2880 2445 Size: 11 424 5356 Fragments arranged by size: 5356 424 11 NcoI C'CATG_G Cuts at: 513 1065 2004 2523 513 Size: 552 939 519 3781 Fragments arranged by size: 3781 939 552 519 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 3149 4694 1132 Size: 97 1920 1545 2229 Fragments arranged by size: 2229 1920 1545 97 PasI CC'CwG_GG Cuts at: 1348 2769 3049 1348 Size: 1421 280 4090 Fragments arranged by size: 4090 1421 280 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2950 1108 Size: 1842 3949 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PmlI CAC'GTG Cuts at: 2019 2035 2728 2019 Size: 16 693 5082 Fragments arranged by size: 5082 693 16 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2268 2268 Size: 5791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 3027 3067 3027 Size: 40 5751 SacI G_AGCT'C Cuts at: 2420 2478 2420 Size: 58 5733 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SapI GCTCTTCn'nnn_ Cuts at: 2483 2483 Size: 5791 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SexAI A'CCwGG_T Cuts at: 3057 3057 Size: 5791 SfcI C'TryA_G Cuts at: 826 1045 3139 4274 4955 5146 826 Size: 219 2094 1135 681 191 1471 Fragments arranged by size: 2094 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2446 2457 2881 2446 Size: 11 424 5356 Fragments arranged by size: 5356 424 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 2266 3118 1872 Size: 394 852 4545 Fragments arranged by size: 4545 852 394 SmlI C'TyrA_G Cuts at: 820 1017 1943 3895 4796 5058 820 Size: 197 926 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5791 StyI C'CwwG_G Cuts at: 513 1065 2004 2523 2898 3230 513 Size: 552 939 519 375 332 3074 Fragments arranged by size: 3074 939 552 519 375 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2212 2382 2524 3818 3835 3988 4173 Size: 1255 632 170 142 1294 17 153 185 Cuts at: 4173 4618 325 Size: 445 1498 Fragments arranged by size: 1498 1294 1255 632 445 185 170 153 142 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 4037 5749 Size: 274 80 33 51 1443 222 1838 1712 Cuts at: 5749 96 Size: 138 Fragments arranged by size: 1838 1712 1443 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2110 2110 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 2264 3116 1870 Size: 394 852 4545 Fragments arranged by size: 4545 852 394 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI