Restriction map for
pFN21AB8811
(Linear) (Six-base) MAPSORT of: KIBB8811.seq Check: 8800 from: 1 to: 1086
KIBB8811.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 161 1086
Size: 161 925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1086
Size: 1084 2
AflIII A'CryG_T
Cuts at: 0 11 1086
Size: 11 1075
AvaI C'yCGr_G
Cuts at: 0 259 1086
Size: 259 827
BamHI G'GATC_C
Cuts at: 0 248 1086
Size: 248 838
BanI G'GyrC_C
Cuts at: 0 439 450 874 1086
Size: 439 11 424 212
Fragments arranged by size:
439 424 212 11
BanII G_rGCy'C
Cuts at: 0 415 473 1086
Size: 415 58 613
Fragments arranged by size:
613 415 58
BbeI G_GCGC'C
Cuts at: 0 443 454 878 1086
Size: 443 11 424 208
Fragments arranged by size:
443 424 208 11
BbvCI CC'TCA_GC
Cuts at: 0 175 679 1086
Size: 175 504 407
Fragments arranged by size:
504 407 175
BglI GCCn_nnn'nGGC
Cuts at: 0 448 1086
Size: 448 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1086
Size: 988 98
Bpu10I CC'TnA_GC
Cuts at: 0 175 679 1086
Size: 175 504 407
Fragments arranged by size:
504 407 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 473 964 1086
Size: 473 491 122
Fragments arranged by size:
491 473 122
BsaAI yAC'GTr
Cuts at: 0 14 30 723 1086
Size: 14 16 693 363
Fragments arranged by size:
693 363 16 14
BsaHI Gr'CG_yC
Cuts at: 0 158 440 451 875 1086
Size: 158 282 11 424 211
Fragments arranged by size:
424 282 211 158 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 796 1086
Size: 796 290
BseYI C'CCAG_C
Cuts at: 0 153 435 1022 1086
Size: 153 282 587 64
Fragments arranged by size:
587 282 153 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 217 886 1078 1086
Size: 186 31 669 192 8
Fragments arranged by size:
669 192 186 31 8
BsiHKAI G_wGCw'C
Cuts at: 0 415 473 728 1086
Size: 415 58 255 358
Fragments arranged by size:
415 358 255 58
Bsp1286I G_dGCh'C
Cuts at: 0 415 473 728 1086
Size: 415 58 255 358
Fragments arranged by size:
415 358 255 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 917 1086
Size: 197 720 169
Fragments arranged by size:
720 197 169
BsrGI T'GTAC_A
Cuts at: 0 194 1086
Size: 194 892
BstAPI GCAn_nnn'nTGC
Cuts at: 0 909 1086
Size: 909 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 435 699 753 1086
Size: 108 327 264 54 333
Fragments arranged by size:
333 327 264 108 54
BstYI r'GATC_y
Cuts at: 0 248 1086
Size: 248 838
BtgI C'CryG_G
Cuts at: 0 252 401 416 428 518 577 1086
Size: 252 149 15 12 90 59 509
Fragments arranged by size:
509 252 149 90 59 15 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 165 297 672 899 1086
Size: 165 132 375 227 187
Fragments arranged by size:
375 227 187 165 132
EaeI y'GGCC_r
Cuts at: 0 404 890 923 947 1028 1086
Size: 404 486 33 24 81 58
Fragments arranged by size:
486 404 81 58 33 24
EarI CTCTTCn'nnn_
Cuts at: 0 478 577 1086
Size: 478 99 509
Fragments arranged by size:
509 478 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 47 856 1025 1086
Size: 47 809 169 61
Fragments arranged by size:
809 169 61 47
EcoICRI GAG'CTC
Cuts at: 0 413 471 1086
Size: 413 58 615
Fragments arranged by size:
615 413 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1084 1086
Size: 988 96 2
Fragments arranged by size:
988 96 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 987 1086
Size: 987 99
EcoO109I rG'GnC_Cy
Cuts at: 0 263 897 1086
Size: 263 634 189
Fragments arranged by size:
634 263 189
HaeII r_GCGC'y
Cuts at: 0 443 454 586 648 746 878 1086
Size: 443 11 132 62 98 132 208
Fragments arranged by size:
443 208 132 132 98 62 11
HincII GTy'rAC
Cuts at: 0 867 1086
Size: 867 219
KasI G'GCGC_C
Cuts at: 0 439 450 874 1086
Size: 439 11 424 212
Fragments arranged by size:
439 424 212 11
MscI TGG'CCA
Cuts at: 0 892 925 949 1086
Size: 892 33 24 137
Fragments arranged by size:
892 137 33 24
MslI CAynn'nnrTG
Cuts at: 0 85 334 1086
Size: 85 249 752
Fragments arranged by size:
752 249 85
MspA1I CmG'CkG
Cuts at: 0 213 1022 1062 1086
Size: 213 809 40 24
Fragments arranged by size:
809 213 40 24
NarI GG'CG_CC
Cuts at: 0 440 451 875 1086
Size: 440 11 424 211
Fragments arranged by size:
440 424 211 11
NcoI C'CATG_G
Cuts at: 0 518 1086
Size: 518 568
PasI CC'CwG_GG
Cuts at: 0 764 1044 1086
Size: 764 280 42
Fragments arranged by size:
764 280 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1086
Size: 945 141
PmlI CAC'GTG
Cuts at: 0 14 30 723 1086
Size: 14 16 693 363
Fragments arranged by size:
693 363 16 14
PpuMI rG'GwC_Cy
Cuts at: 0 263 1086
Size: 263 823
PvuII CAG'CTG
Cuts at: 0 1022 1062 1086
Size: 1022 40 24
Fragments arranged by size:
1022 40 24
SacI G_AGCT'C
Cuts at: 0 415 473 1086
Size: 415 58 613
Fragments arranged by size:
613 415 58
SapI GCTCTTCn'nnn_
Cuts at: 0 478 1086
Size: 478 608
SexAI A'CCwGG_T
Cuts at: 0 1052 1086
Size: 1052 34
SfoI GGC'GCC
Cuts at: 0 441 452 876 1086
Size: 441 11 424 210
Fragments arranged by size:
441 424 210 11
SmaI CCC'GGG
Cuts at: 0 261 1086
Size: 261 825
StyI C'CwwG_G
Cuts at: 0 518 893 1086
Size: 518 375 193
Fragments arranged by size:
518 375 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 207 377 519 1086
Size: 207 170 142 567
Fragments arranged by size:
567 207 170 142
TatI w'GTAC_w
Cuts at: 0 194 1086
Size: 194 892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 105 1086
Size: 105 981
XmaI C'CCGG_G
Cuts at: 0 259 1086
Size: 259 827
Enzymes that do cut:
AccI AcuI AflIII AvaI BamHI BanI BanII BbeI
BbvCI BglI BpmI Bpu10I BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrGI BstAPI BstXI
BstYI BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII HincII KasI MscI MslI MspA1I
NarI NcoI PasI PflMI PmlI PpuMI PvuII SacI
SapI SexAI SfoI SmaI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI