Restriction map for
pFN21AB8810
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CelII Cfr10I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MluI
MluNI MroI MscI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AfaI 616 GTAC GT^AC
AluI 812 AGCT AG^CT
Alw44I 400 GTGCAC G^TGCAC
AlwNI 1010 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 46 CCTCAGG CC^T[ACGT]AGG
ApaLI 400 GTGCAC G^TGCAC
AspHI 400 GTGCAC G[AT]GC[AT]^C
AvaII 909 GGACC G^G[AT]CC
BanII 133 GGGCTC G[GA]GC[CT]^C
BbrPI 1028 CACGTG CAC^GTG
BciVI 549 GTATCC GTATCC(6/5)
BglII 653 AGATCT A^GATCT
BpmI 288 CTGGAG CTGGAG(16/14)
BsaAI 1028 CACGTG [CT]AC^GT[GA]
BsgI 868 CTGCAC GTGCAG(16/14)
BsiHKAI 400 GTGCAC G[AT]GC[AT]^C
BsiYI 305 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 305 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 987 ATCGAT AT^CGAT
BsrI 287 ACTGG ACTGG(1/-1)
BssSI 136 CTCGTG CACGAG(-5/-1)
BstF5I 962 GGATG GGATG(2/0)
BstYI 653 AGATCT [GA]^GATC[CT]
Bsu36I 46 CCTCAGG CC^T[ACGT]AGG
CfoI 322 GCGC GCG^C
Cfr13I 909 GGACC G^G[ACGT]CC
ClaI 987 ATCGAT AT^CGAT
DpnII 654 GATC ^GATC
EaeI 86 CGGCCA [CT]^GGCC[GA]
Eco81I 46 CCTCAGG CC^T[ACGT]AGG
EcoNI 305 CCTTGTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 155 CCTTGG C^C[AT][AT]GG
EcoT22I 540 ATGCAT ATGCA^T
FokI 962 GGATG GGATG(9/13)
HaeIII 87 GGCC GG^CC
HhaI 322 GCGC GCG^C
HinP1I 322 GCGC G^CGC
HindIII 811 AAGCTT A^AGCTT
HphI 219 GGTGA GGTGA(8/7)
MaeII 1029 ACGT A^CGT
MaeIII 62 GTGAC ^GT[ACGT]AC
MboI 654 GATC ^GATC
MfeI 897 CAATTG C^AATTG
MflI 653 AGATCT [GA]^GATC[CT]
MseI 806 TTAA T^TAA
MslI 390 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 897 CAATTG C^AATTG
NdeII 654 GATC ^GATC
NlaIV 909 GGACCC GG[ACGT]^[ACGT]CC
NsiI 540 ATGCAT ATGCA^T
PmaCI 1028 CACGTG CAC^GTG
PmlI 1028 CACGTG CAC^GTG
RsaI 616 GTAC GT^AC
Sau3AI 654 GATC ^GATC
Sau96I 909 GGACC G^G[ACGT]CC
StyI 155 CCTTGG C^C[AT][AT]GG
TaqI 988 TCGA T^CGA
Tru9I 806 TTAA T^TAA
Tsp45I 62 GTGAC ^GT[GC]AC
TthHB8I 988 TCGA T^CGA
XhoII 653 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 213 AAATTT [GA]^AATT[CT]
1023 AAATTC
ApoI 213 AAATTT [GA]^AATT[CT]
1023 AAATTC
BbsI 121 GTCTTC GAAGAC(2/6)
1068 GTCTTC
BbvI 659 GCTGC GCAGC(8/12)
902 GCTGC
BmyI 133 GGGCTC G[AGT]GC[ACT]^C
400 GTGCAC
BpuAI 121 GTCTTC GAAGAC(2/6)
1068 GTCTTC
BsaJI 155 CCTTGG C^C[ACGT][ACGT]GG
580 CCCTGG
BseRI 44 CTCCTC GAGGAG(10/8)
496 CTCCTC
Bsp1286I 133 GGGCTC G[AGT]GC[ACT]^C
400 GTGCAC
BspMI 367 ACCTGC ACCTGC(4/8)
349 GCAGGT
BsrDI 390 CATTGC GCAATG(2/0)
274 GCAATG
EarI 575 CTCTTC CTCTTC(1/4)
667 CTCTTC
Fnu4HI 659 GCTGC GC^[ACGT]GC
902 GCTGC
ItaI 659 GCTGC GC^[ACGT]GC
902 GCTGC
Ksp632I 575 CTCTTC CTCTTC(1/4)
667 CTCTTC
MwoI 265 GCCTACTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
966 GCTGAAACGGC
NdeI 344 CATATG CA^TATG
799 CATATG
SfaNI 301 GATGC GCATC(5/9)
963 GATGC
TseI 659 GCTGC G^C[AT]GC
902 GCTGC
TspRI 696 CACTG CA[GC]TG[ACGT][ACGT]^
981 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BfaI BssKI BstNI DdeI
EcoRII HinfI MaeI MboII MnlI
MvaI NlaIII NspI ScrFI TfiI
Tsp509I