(Circular) (Six-base) MAPSORT of: pFN21AB8810.seq Check: 5476 from: 1 to: 5788 pFN21AB8810 5788 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2345 2345 Size: 5788 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2659 3042 3851 5235 5503 1632 Size: 1027 383 809 1384 268 1917 Fragments arranged by size: 1917 1384 1027 809 383 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3020 3531 5103 3020 Size: 511 1572 3705 Fragments arranged by size: 3705 1572 511 ApaLI G'TGCA_C Cuts at: 2405 3845 5001 2405 Size: 1440 1156 3192 Fragments arranged by size: 3192 1440 1156 ApoI r'AATT_y Cuts at: 1493 2218 3028 3097 3323 3475 5584 1493 Size: 725 810 69 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 725 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 3113 1870 Size: 73 1170 4545 Fragments arranged by size: 4545 1170 73 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 4564 618 Size: 325 217 627 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 3107 1391 Size: 751 965 4072 Fragments arranged by size: 4072 965 751 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 3066 928 Size: 971 220 947 3650 Fragments arranged by size: 3650 971 947 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2565 3680 4890 2565 Size: 1115 1210 3463 Fragments arranged by size: 3463 1210 1115 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BfrBI ATG'CAT Cuts at: 2547 2547 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2658 5783 2658 Size: 3125 2663 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2409 3849 5005 1790 Size: 619 1440 1156 2573 Fragments arranged by size: 2573 1440 1156 619 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2314 4448 1239 Size: 1075 2134 2579 Fragments arranged by size: 2579 2134 1075 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3035 3579 493 Size: 711 1831 544 2702 Fragments arranged by size: 2702 1831 711 544 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2484 2514 1178 Size: 30 165 30 1081 30 4452 Fragments arranged by size: 4452 1081 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2492 1186 Size: 854 452 4482 Fragments arranged by size: 4482 854 452 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2858 1163 Size: 573 1122 4093 Fragments arranged by size: 4093 1122 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 3849 3934 5005 2409 Size: 1440 85 1071 3192 Fragments arranged by size: 3192 1440 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2409 3107 3849 3934 5005 1391 Size: 399 352 267 698 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 399 352 267 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 2381 3143 844 Size: 1501 36 762 3489 Fragments arranged by size: 3489 1501 762 36 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2286 2394 4283 4457 65 Size: 1183 1038 108 1889 174 1396 Fragments arranged by size: 1889 1396 1183 1038 174 108 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 2141 3848 4860 2141 Size: 1707 1012 3069 Fragments arranged by size: 3069 1707 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2658 3118 3485 3870 3887 5339 5425 5437 Size: 686 460 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 686 460 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2052 2052 Size: 5788 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 2993 1554 Size: 1439 4349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3235 4124 Size: 54 1425 50 275 18 261 1144 889 Cuts at: 4124 5753 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1144 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 2678 3718 1353 Size: 1233 92 1040 3423 Fragments arranged by size: 3423 1233 1040 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2025 4375 4749 4895 387 Size: 1064 138 436 2350 374 146 1280 Fragments arranged by size: 2350 1280 1064 436 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2314 2659 3042 3851 4448 5235 5503 Size: 393 682 345 383 809 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 809 787 682 597 393 383 345 268 EcoNI CCTnn'n_nnAGG Cuts at: 2314 2314 Size: 5788 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373 Size: 32 135 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 2816 748 Size: 2068 3720 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 2902 3387 2902 Size: 485 5303 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4902 5086 1305 Size: 3597 184 2007 Fragments arranged by size: 3597 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 3746 4105 4264 518 Size: 648 1233 1347 359 159 2042 Fragments arranged by size: 2042 1347 1233 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3204 3881 5029 5274 1606 Size: 1598 677 1148 245 2120 Fragments arranged by size: 2120 1598 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 NdeI CA'TA_TG Cuts at: 387 2350 2805 387 Size: 1963 455 3370 Fragments arranged by size: 3370 1963 455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NsiI A_TGCA'T Cuts at: 2549 2549 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2447 2547 2607 2940 3146 4691 1132 Size: 97 1218 100 60 333 206 1545 2229 Fragments arranged by size: 2229 1545 1218 333 206 100 97 60 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PfoI T'CCnGG_A Cuts at: 2835 2835 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PmlI CAC'GTG Cuts at: 3035 3035 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 2633 3358 2633 Size: 725 5063 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 3136 4271 4952 5143 826 Size: 219 2091 1135 681 191 1471 Fragments arranged by size: 2091 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 3227 513 Size: 552 939 156 1067 3074 Fragments arranged by size: 3074 1067 939 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 4034 5746 Size: 274 80 33 51 1443 643 1414 1712 Cuts at: 5746 96 Size: 138 Fragments arranged by size: 1712 1443 1414 643 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 5748 5780 2484 Size: 32 3232 32 2492 Fragments arranged by size: 3232 2492 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI