Restriction map for
pFN21AB8810
(Linear) (Six-base) MAPSORT of: KIBB8810.seq Check: 8879 from: 1 to: 1083
KIBB8810.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 340 1083
Size: 340 743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1037 1083
Size: 654 383 46
Fragments arranged by size:
654 383 46
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1083
Size: 1015 68
ApaLI G'TGCA_C
Cuts at: 0 400 1083
Size: 400 683
ApoI r'AATT_y
Cuts at: 0 213 1023 1083
Size: 213 810 60
Fragments arranged by size:
810 213 60
BanII G_rGCy'C
Cuts at: 0 137 1083
Size: 137 946
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 1061 1083
Size: 114 947 22
Fragments arranged by size:
947 114 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 560 1083
Size: 560 523
BfrBI ATG'CAT
Cuts at: 0 542 1083
Size: 542 541
BglII A'GATC_T
Cuts at: 0 653 1083
Size: 653 430
Bme1580I G_kGCm'C
Cuts at: 0 404 1083
Size: 404 679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 1083
Size: 309 774
BsaAI yAC'GTr
Cuts at: 0 1030 1083
Size: 1030 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 479 509 1083
Size: 479 30 574
Fragments arranged by size:
574 479 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 487 1083
Size: 35 452 596
Fragments arranged by size:
596 452 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1083
Size: 853 230
BsiHKAI G_wGCw'C
Cuts at: 0 404 1083
Size: 404 679
Bsp1286I G_dGCh'C
Cuts at: 0 137 404 1083
Size: 137 267 679
Fragments arranged by size:
679 267 137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 376 1083
Size: 340 36 707
Fragments arranged by size:
707 340 36
BsrDI GCAATG_nn'
Cuts at: 0 281 389 1083
Size: 281 108 694
Fragments arranged by size:
694 281 108
BssSI C'ACGA_G
Cuts at: 0 136 1083
Size: 136 947
BstYI r'GATC_y
Cuts at: 0 653 1083
Size: 653 430
Bsu36I CC'TnA_GG
Cuts at: 0 47 1083
Size: 47 1036
ClaI AT'CG_AT
Cuts at: 0 988 1083
Size: 988 95
EaeI y'GGCC_r
Cuts at: 0 86 1083
Size: 86 997
EarI CTCTTCn'nnn_
Cuts at: 0 581 673 1083
Size: 581 92 410
Fragments arranged by size:
581 410 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 20 1083
Size: 20 1063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 654 1037 1083
Size: 309 345 383 46
Fragments arranged by size:
383 345 309 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 309 1083
Size: 309 774
HindIII A'AGCT_T
Cuts at: 0 811 1083
Size: 811 272
MfeI C'AATT_G
Cuts at: 0 897 1083
Size: 897 186
MslI CAynn'nnrTG
Cuts at: 0 394 1083
Size: 394 689
NdeI CA'TA_TG
Cuts at: 0 345 800 1083
Size: 345 455 283
Fragments arranged by size:
455 345 283
NsiI A_TGCA'T
Cuts at: 0 544 1083
Size: 544 539
NspI r_CATG'y
Cuts at: 0 442 542 602 935 1083
Size: 442 100 60 333 148
Fragments arranged by size:
442 333 148 100 60
PfoI T'CCnGG_A
Cuts at: 0 830 1083
Size: 830 253
PmlI CAC'GTG
Cuts at: 0 1030 1083
Size: 1030 53
PsiI TTA'TAA
Cuts at: 0 628 1083
Size: 628 455
StyI C'CwwG_G
Cuts at: 0 155 1083
Size: 155 928
TatI w'GTAC_w
Cuts at: 0 615 1083
Size: 615 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 1083
Size: 479 32 572
Fragments arranged by size:
572 479 32
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI BanII BbsI BciVI
BfrBI BglII Bme1580I BpmI BsaAI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstYI Bsu36I ClaI
EaeI EarI EciI Eco57MI EcoNI HindIII MfeI MslI
NdeI NsiI NspI PfoI PmlI PsiI StyI TatI
TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EagI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI