Restriction map for
pFN21AB8808
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaI AviII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI HpaII KasI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 392 GGTACC G^GTACC
AciI 479 CCGC CCGC(-3/-1)
AfaI 393 GTAC GT^AC
AlwI 624 GATCC GGATC(4/5)
Asp718I 392 GGTACC G^GTACC
AspHI 718 GTGCTC G[AT]GC[AT]^C
BanI 392 GGTACC G^G[CT][GA]CC
BbvCI 195 GCTGAGG CCTCAGC(-5/-2)
BglI 481 GCCATTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 584 AGATCT A^GATCT
BpmI 1044 CTCCAG CTGGAG(16/14)
BsgI 664 CTGCAC GTGCAG(16/14)
BsiHKAI 718 GTGCTC G[AT]GC[AT]^C
BspHI 377 TCATGA T^CATGA
BspMI 242 ACCTGC ACCTGC(4/8)
BsrI 291 CCAGT ACTGG(1/-1)
Bst1107I 214 GTATAC GTA^TAC
BstAPI 789 GCACAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 707 CCATCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 584 AGATCT [GA]^GATC[CT]
BstZ17I 214 GTATAC GTA^TAC
Cac8I 1022 GCCAGC GC[ACGT]^[ACGT]GC
DraII 752 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 675 GAAGAG CTCTTC(1/4)
EcoO109I 752 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 55 GATATC GAT^ATC
KpnI 392 GGTACC GGTAC^C
Ksp632I 675 GAAGAG CTCTTC(1/4)
MaeII 764 ACGT A^CGT
MflI 584 AGATCT [GA]^GATC[CT]
MseI 656 TTAA T^TAA
NspI 359 GCATGT [GA]CATG^[CT]
PpuMI 752 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 377 TCATGA T^CATGA
RsaI 393 GTAC GT^AC
SmlI 63 CTCAAG C^T[CT][GA]AG
SspI 939 AATATT AAT^ATT
Tru9I 656 TTAA T^TAA
XcmI 291 CCAGTGGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 584 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 214 GTATAC GT^[AC][GT]AC
1051 GTAGAC
AvrII 975 CCTAGG C^CTAGG
991 CCTAGG
BlnI 975 CCTAGG C^CTAGG
991 CCTAGG
BmyI 447 GGGCAC G[AGT]GC[ACT]^C
718 GTGCTC
BseRI 1032 CTCCTC GAGGAG(10/8)
198 GAGGAG
BsiYI 70 CCCTGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCTGTGATGGG
BslI 70 CCCTGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCTGTGATGGG
BsmFI 304 GGGAC GGGAC(10/14)
754 GTCCC
Bsp1286I 447 GGGCAC G[AGT]GC[ACT]^C
718 GTGCTC
BsrDI 500 CATTGC GCAATG(2/0)
732 CATTGC
MspA1I 670 CAGCTG C[AC]G^C[GT]G
829 CAGCTG
PvuII 670 CAGCTG CAG^CTG
829 CAGCTG
SfaNI 203 GCATC GCATC(5/9)
736 GCATC
SfcI 351 CTACAG C^T[GA][CT]AG
541 CTACAG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI Asp700I AvaII BbsI BbvI
BfaI BpuAI BsaJI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI XmnI