(Circular) (Six-base) MAPSORT of: pFN21AB8808.seq Check: 733 from: 1 to: 5770 pFN21AB8808 5770 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 3057 3113 1937 Size: 283 837 56 4594 Fragments arranged by size: 4594 837 283 56 Acc65I G'GTAC_C Cuts at: 2397 3091 2397 Size: 694 5076 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2553 2699 3833 5217 5485 1632 Size: 921 146 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 921 268 146 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 2883 1166 Size: 1717 4053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 AvrII C'CTAG_G Cuts at: 2980 2996 2980 Size: 16 5754 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 3091 4546 618 Size: 325 217 627 610 694 1455 1842 Fragments arranged by size: 1842 1455 694 627 610 325 217 BanII G_rGCy'C Cuts at: 1391 3089 1391 Size: 1698 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 2251 2992 928 Size: 971 245 107 741 3706 Fragments arranged by size: 3706 971 741 245 107 BbvCI CC'TCA_GC Cuts at: 2201 2201 Size: 5770 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2492 4381 136 Size: 107 122 71 631 605 820 1889 1525 Fragments arranged by size: 1889 1525 820 631 605 122 107 71 BglII A'GATC_T Cuts at: 2589 5765 2589 Size: 3176 2594 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2456 3831 4987 1790 Size: 666 1375 1156 2573 Fragments arranged by size: 2573 1375 1156 666 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3034 4430 1239 Size: 1795 1396 2579 Fragments arranged by size: 2579 1795 1396 Bpu10I CC'TnA_GC Cuts at: 2201 2201 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2053 3895 4760 5058 2053 Size: 1842 865 298 2765 Fragments arranged by size: 2765 1842 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2218 3028 1186 Size: 1032 810 3928 Fragments arranged by size: 3928 1032 810 BseYI C'CCAG_C Cuts at: 4973 5469 4973 Size: 496 5274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2654 1163 Size: 573 918 4279 Fragments arranged by size: 4279 918 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 3831 3916 4987 2727 Size: 1104 85 1071 3510 Fragments arranged by size: 3510 1104 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2456 2727 3089 3831 3916 4987 1391 Size: 399 666 271 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 666 399 362 271 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 2382 3659 5389 2382 Size: 1277 1730 2763 Fragments arranged by size: 2763 1730 1277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2256 3125 844 Size: 1412 869 3489 Fragments arranged by size: 3489 1412 869 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2504 2736 4265 4439 65 Size: 1183 1256 232 1529 174 1396 Fragments arranged by size: 1529 1396 1256 1183 232 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4414 4601 1700 Size: 110 2604 187 2869 Fragments arranged by size: 2869 2604 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 2800 794 Size: 2006 3764 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2719 2719 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2589 3100 3467 3852 3869 5321 5407 5419 Size: 617 511 367 385 17 1452 86 12 Cuts at: 5419 5765 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 617 511 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2221 2221 Size: 5770 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 2878 3286 4080 4100 2878 Size: 408 794 20 4548 Fragments arranged by size: 4548 794 408 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735 Size: 54 1425 50 275 18 1387 889 1629 Cuts at: 5735 8 Size: 43 Fragments arranged by size: 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2675 3700 1353 Size: 1322 1025 3423 Fragments arranged by size: 3423 1322 1025 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 2699 3034 3833 4430 5217 5485 Size: 393 921 146 335 799 597 787 268 Cuts at: 5485 1239 Size: 1524 Fragments arranged by size: 1524 921 799 787 597 393 335 268 146 EcoO109I rG'GnC_Cy Cuts at: 2758 2758 Size: 5770 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 EcoRV GAT'ATC Cuts at: 2062 2062 Size: 5770 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2269 2301 4411 Size: 32 135 32 479 32 186 32 2110 Cuts at: 4411 4443 4485 4517 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2110 479 186 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 2401 3095 2401 Size: 694 5076 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2387 2645 2883 3728 4087 4246 Size: 648 1215 6 258 238 845 359 159 Cuts at: 4246 518 Size: 2042 Fragments arranged by size: 2042 1215 845 648 359 258 238 159 6 MspA1I CmG'CkG Cuts at: 1606 2677 2836 3186 3863 5011 5256 1606 Size: 1071 159 350 677 1148 245 2120 Fragments arranged by size: 2120 1148 1071 677 350 245 159 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2368 3128 4673 1132 Size: 97 1139 760 1545 2229 Fragments arranged by size: 2229 1545 1139 760 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 2758 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2677 2836 2677 Size: 159 5611 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SanDI GG'GwC_CC Cuts at: 2758 2758 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 2356 2546 3118 4253 4934 5125 826 Size: 219 1311 190 572 1135 681 191 1471 Fragments arranged by size: 1471 1311 1135 681 572 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2068 3874 4775 5037 820 Size: 197 926 125 1806 901 262 1553 Fragments arranged by size: 1806 1553 926 901 262 197 125 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 2946 3694 5 Size: 47 2894 748 2081 Fragments arranged by size: 2894 2081 748 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2706 2980 2996 3209 513 Size: 552 939 702 274 16 213 3074 Fragments arranged by size: 3074 939 702 552 274 213 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2057 2290 2864 3797 3814 3967 4152 Size: 1255 477 233 574 933 17 153 185 Cuts at: 4152 4597 325 Size: 445 1498 Fragments arranged by size: 1498 1255 933 574 477 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4016 5728 96 Size: 274 80 33 51 1443 2039 1712 138 Fragments arranged by size: 2039 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2303 2303 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 2091 2591 2946 3899 2091 Size: 500 355 953 3962 Fragments arranged by size: 3962 953 500 355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI