(Circular) (Six-base) MAPSORT of: pFN21AB8803.seq Check: 7827 from: 1 to: 5752 pFN21AB8803 5752 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 2703 3815 5199 5467 1632 Size: 881 190 1112 1384 268 1917 Fragments arranged by size: 1917 1384 1112 881 268 190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 2555 4481 2555 Size: 1926 3826 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 2745 Size: 32 5720 AlwNI CAG_nnn'CTG Cuts at: 3034 3495 5067 3034 Size: 461 1572 3719 Fragments arranged by size: 3719 1572 461 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2178 3061 3287 3439 5548 1493 Size: 685 883 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 685 226 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2121 3077 1870 Size: 73 178 956 4545 Fragments arranged by size: 4545 956 178 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 2481 2713 3071 1391 Size: 1090 232 358 4072 Fragments arranged by size: 4072 1090 358 232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4781 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2777 4363 136 Size: 107 122 71 631 605 1105 1586 1525 Fragments arranged by size: 1586 1525 1105 631 605 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2544 2657 3813 4969 1790 Size: 754 113 1156 1156 2573 Fragments arranged by size: 2573 1156 1156 754 113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2990 3483 4441 Size: 416 53 83 399 24 1573 493 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1573 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 2733 4412 1239 Size: 1388 106 1679 2579 Fragments arranged by size: 2579 1679 1388 106 Bpu10I CC'TnA_GC Cuts at: 3027 3027 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 2178 2568 2915 3877 4742 5040 2165 Size: 13 390 347 962 865 298 2877 Fragments arranged by size: 2877 962 865 390 347 298 13 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2118 2264 4415 882 Size: 617 619 146 2151 2219 Fragments arranged by size: 2219 2151 619 617 146 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 2330 5746 2330 Size: 3416 2336 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3559 3941 4576 Size: 53 83 186 936 479 1547 382 635 Cuts at: 4576 275 Size: 1451 Fragments arranged by size: 1547 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 1178 Size: 30 165 30 1086 30 4411 Fragments arranged by size: 4411 1086 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5752 BseYI C'CCAG_C Cuts at: 2672 4955 5451 2672 Size: 2283 496 2973 Fragments arranged by size: 2973 2283 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 3813 3898 4969 2481 Size: 1332 85 1071 3264 Fragments arranged by size: 3264 1332 1071 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2481 2544 2657 2713 3071 3813 3898 Size: 399 691 63 113 56 358 742 85 Cuts at: 3898 4969 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 358 113 85 63 56 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2845 3639 1394 Size: 429 1022 794 3507 Fragments arranged by size: 3507 1022 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2424 2424 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2164 2950 3082 3449 3834 3851 5303 5389 Size: 192 786 132 367 385 17 1452 86 Cuts at: 5389 5401 5747 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 786 385 367 346 192 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2335 2341 2453 513 Size: 552 939 331 6 112 3812 Fragments arranged by size: 3812 939 552 331 112 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2045 2080 529 Size: 35 1481 35 4201 Fragments arranged by size: 4201 1481 35 35 DraI TTT'AAA Cuts at: 2862 3056 2862 Size: 194 5558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2338 2631 2854 Size: 54 1425 50 275 18 508 293 223 Cuts at: 2854 3199 4088 5717 8 Size: 345 889 1629 43 Fragments arranged by size: 1629 1425 889 508 345 293 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 3682 1353 Size: 1365 964 3423 Fragments arranged by size: 3423 1365 964 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2479 2479 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2627 2703 2733 3815 4412 5199 Size: 393 881 114 76 30 1082 597 787 Cuts at: 5199 5467 1239 Size: 268 1524 Fragments arranged by size: 1524 1082 881 787 597 393 268 114 76 30 EcoNI CCTnn'n_nnAGG Cuts at: 2933 2933 Size: 5752 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2543 2543 Size: 5752 FspI TGC'GCA Cuts at: 3038 4258 3038 Size: 1220 4532 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2541 2573 4393 4425 4467 Size: 32 135 32 969 32 1820 32 42 Cuts at: 4467 4499 1373 Size: 32 2626 Fragments arranged by size: 2626 1820 969 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 2280 748 Size: 1532 4220 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 2028 3351 2028 Size: 1323 4429 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 4866 5050 1305 Size: 1515 2046 184 2007 Fragments arranged by size: 2046 2007 1515 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2340 2633 2856 10 Size: 54 1425 343 508 293 223 2906 Fragments arranged by size: 2906 1425 508 343 293 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 3710 4069 4228 518 Size: 648 2544 359 159 2042 Fragments arranged by size: 2544 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2533 3168 3845 4993 5238 1606 Size: 927 635 677 1148 245 2120 Fragments arranged by size: 2120 1148 927 677 635 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2341 2453 513 Size: 552 939 337 112 3812 Fragments arranged by size: 3812 939 552 337 112 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 1108 Size: 1239 4513 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2533 2533 Size: 5752 SacI G_AGCT'C Cuts at: 2481 2481 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2718 2718 Size: 5752 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SexAI A'CCwGG_T Cuts at: 2740 2740 Size: 5752 SfcI C'TryA_G Cuts at: 826 1045 3100 4235 4916 5107 826 Size: 219 2055 1135 681 191 1471 Fragments arranged by size: 2055 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 2193 2583 2930 3856 4757 Size: 197 926 201 49 390 347 926 901 Cuts at: 4757 5019 820 Size: 262 1553 Fragments arranged by size: 1553 926 926 901 390 347 262 201 197 49 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2867 1517 Size: 1350 4402 StyI C'CwwG_G Cuts at: 513 1065 2004 2341 2453 2899 3191 513 Size: 552 939 337 112 446 292 3074 Fragments arranged by size: 3074 939 552 446 337 292 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 3796 3949 4134 4579 325 Size: 1255 2199 17 153 185 445 1498 Fragments arranged by size: 2199 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3998 5710 96 Size: 274 80 33 51 1443 2021 1712 138 Fragments arranged by size: 2021 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 5712 5744 2489 Size: 32 3191 32 2497 Fragments arranged by size: 3191 2497 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 2182 3088 2182 Size: 906 4846 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2897 2897 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 2664 3881 2664 Size: 1217 4535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI