Restriction map for
pFN21AB8798
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssKI BssSI BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstYI Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MflI MluI MroI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI Sau96I
SbfI ScrFI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 514 GTATAC GT^[AC][GT]AC
AciI 348 CCGC CCGC(-3/-1)
AcsI 433 AAATTT [GA]^AATT[CT]
AflIII 16 ACATGT A^C[GA][CT]GT
ApoI 433 AAATTT [GA]^AATT[CT]
Asp700I 228 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 417 TGGCCA TGG^CCA
BbsI 951 GAAGAC GAAGAC(2/6)
BpuAI 951 GAAGAC GAAGAC(2/6)
BsaI 607 GGTCTC GGTCTC(1/5)
BsaJI 44 CCATGG C^C[ACGT][ACGT]GG
BsgI 192 GTGCAG GTGCAG(16/14)
BspHI 209 TCATGA T^CATGA
BspLU11I 16 ACATGT A^CATGT
BspMI 604 GCAGGT ACCTGC(4/8)
Bst1107I 514 GTATAC GTA^TAC
BstZ17I 514 GTATAC GTA^TAC
DraI 802 TTTAAA TTT^AAA
DraIII 166 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 44 CCATGG C^C[GA][CT]GG
EcoT14I 44 CCATGG C^C[AT][AT]GG
Fnu4HI 347 GCCGC GC^[ACGT]GC
HphI 881 TCACC GGTGA(8/7)
ItaI 347 GCCGC GC^[ACGT]GC
MaeII 703 ACGT A^CGT
MfeI 847 CAATTG C^AATTG
MluNI 417 TGGCCA TGG^CCA
MscI 417 TGGCCA TGG^CCA
MunI 847 CAATTG C^AATTG
NcoI 44 CCATGG C^CATGG
RcaI 209 TCATGA T^CATGA
ScaI 648 AGTACT AGT^ACT
SfcI 536 CTACAG C^T[GA][CT]AG
SphI 297 GCATGC GCATG^C
SspI 408 AATATT AAT^ATT
StyI 44 CCATGG C^C[AT][AT]GG
TspRI 195 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 228 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BfaI 455 CTAG C^TAG
580 CTAG
BsrI 489 ACTGG ACTGG(1/-1)
505 CCAGT
BstF5I 66 CATCC GGATG(2/0)
759 CATCC
Cac8I 297 GCATGC GC[ACGT]^[ACGT]GC
440 GCCAGC
DpnII 36 GATC ^GATC
591 GATC
EaeI 417 TGGCCA [CT]^GGCC[GA]
896 CGGCCA
EarI 39 CTCTTC CTCTTC(1/4)
547 GAAGAG
EcoT22I 295 ATGCAT ATGCA^T
465 ATGCAT
FokI 66 CATCC GGATG(9/13)
759 CATCC
HaeIII 418 GGCC GG^CC
897 GGCC
HinfI 373 GATTC G^A[ACGT]TC
765 GATTC
Ksp632I 39 CTCTTC CTCTTC(1/4)
547 GAAGAG
MaeI 455 CTAG C^TAG
580 CTAG
MboI 36 GATC ^GATC
591 GATC
NdeII 36 GATC ^GATC
591 GATC
NlaIV 583 GGAGCC GG[ACGT]^[ACGT]CC
616 GGAACC
NsiI 295 ATGCAT ATGCA^T
465 ATGCAT
NspI 16 ACATGT [GA]CATG^[CT]
297 GCATGC
Sau3AI 36 GATC ^GATC
591 GATC
TfiI 373 GATTC G^A[AT]TC
765 GATTC
Tsp45I 162 GTCAC ^GT[GC]AC
172 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AfaI BsmAI DdeI MaeIII MboII
MnlI MseI NdeI NlaIII RsaI
Tru9I Tsp509I