(Circular) (Six-base) MAPSORT of: pFN21AB8797.seq Check: 259 from: 1 to: 5704 pFN21AB8797 5704 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2262 2983 2262 Size: 721 4983 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2193 2442 4603 5580 1203 Size: 990 249 2161 977 1327 Fragments arranged by size: 2161 1327 990 977 249 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 2576 1166 Size: 1410 4294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 1184 Size: 1017 4687 AlwNI CAG_nnn'CTG Cuts at: 2068 2561 2998 3447 5019 2068 Size: 493 437 449 1572 2753 Fragments arranged by size: 2753 1572 493 449 437 ApaLI G'TGCA_C Cuts at: 2249 2397 2722 3761 4917 2249 Size: 148 325 1039 1156 3036 Fragments arranged by size: 3036 1156 1039 325 148 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2266 3029 1870 Size: 73 323 763 4545 Fragments arranged by size: 4545 763 323 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 3025 4480 618 Size: 325 217 627 809 429 1455 1842 Fragments arranged by size: 1842 1455 809 627 429 325 217 BanII G_rGCy'C Cuts at: 1391 2273 2691 2739 3023 1391 Size: 882 418 48 284 4072 Fragments arranged by size: 4072 882 418 284 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4315 136 Size: 107 122 71 631 605 2643 1525 Fragments arranged by size: 2643 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2231 2372 3121 2231 Size: 141 749 4814 Fragments arranged by size: 4814 749 141 Bme1580I G_kGCm'C Cuts at: 1790 2253 2401 2726 2772 3765 4921 1790 Size: 463 148 325 46 993 1156 2573 Fragments arranged by size: 2573 1156 993 463 325 148 46 BmgBI CAC'GTC Cuts at: 1318 2194 1318 Size: 876 4828 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2705 2848 4364 1239 Size: 1466 143 1516 2579 Fragments arranged by size: 2579 1516 1466 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2311 2443 2593 3495 493 Size: 711 1107 132 150 902 2702 Fragments arranged by size: 2702 1107 902 711 150 132 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2568 2744 3511 3893 Size: 53 83 186 936 1035 176 767 382 Cuts at: 3893 4528 275 Size: 635 1451 Fragments arranged by size: 1451 1035 936 767 635 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2476 1186 Size: 874 416 4414 Fragments arranged by size: 4414 874 416 BseYI C'CCAG_C Cuts at: 2074 2098 2764 2915 4907 5403 2074 Size: 24 666 151 1992 496 2375 Fragments arranged by size: 2375 1992 666 496 151 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 3008 1163 Size: 573 1074 198 3859 Fragments arranged by size: 3859 1074 573 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2253 2273 2401 2726 3765 3850 4921 2232 Size: 21 20 128 325 1039 85 1071 3015 Fragments arranged by size: 3015 1071 1039 325 128 85 21 20 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2253 2273 2401 2691 2726 2739 Size: 399 442 21 20 128 290 35 13 Cuts at: 2739 2772 3023 3765 3850 4921 1391 Size: 33 251 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 290 251 128 85 35 33 21 20 13 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2262 2983 3059 844 Size: 1232 186 721 76 3489 Fragments arranged by size: 3489 1232 721 186 76 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2679 2958 4348 4535 1700 Size: 110 869 279 1390 187 2869 Fragments arranged by size: 2869 1390 869 279 187 110 BsrGI T'GTAC_A Cuts at: 96 2427 96 Size: 2331 3373 BssHII G'CGCG_C Cuts at: 1925 2466 2526 1925 Size: 541 60 5103 Fragments arranged by size: 5103 541 60 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 2561 2995 794 Size: 1767 434 3503 Fragments arranged by size: 3503 1767 434 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2854 2854 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2322 3034 3401 3786 3803 5255 5341 5353 Size: 350 712 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 712 385 367 350 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2405 2799 513 Size: 552 1340 394 3418 Fragments arranged by size: 3418 1340 552 394 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2220 2315 2399 2962 3220 4014 4034 2220 Size: 95 84 563 258 794 20 3890 Fragments arranged by size: 3890 794 563 258 95 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DraIII CAC_nnn'GTG Cuts at: 2721 2833 2721 Size: 112 5592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2349 2418 Size: 54 1425 50 275 18 324 195 69 Cuts at: 2418 2607 2712 3151 4040 5669 8 Size: 189 105 439 889 1629 43 Fragments arranged by size: 1629 1425 889 439 324 275 195 189 105 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 3634 1353 Size: 1148 1133 3423 Fragments arranged by size: 3423 1148 1133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2271 2271 Size: 5704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2705 2848 3767 4364 5151 5419 1239 Size: 393 1073 143 919 597 787 268 1524 Fragments arranged by size: 1524 1073 919 787 597 393 268 143 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 5704 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2238 2285 4851 1678 Size: 560 47 2566 2531 Fragments arranged by size: 2566 2531 560 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373 Size: 32 135 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3048 3294 669 Size: 988 281 268 842 246 3079 Fragments arranged by size: 3079 988 842 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2950 4818 5002 1305 Size: 1645 1868 184 2007 Fragments arranged by size: 2007 1868 1645 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 2351 2420 2609 2714 Size: 54 1425 343 324 195 69 189 105 Cuts at: 2714 10 Size: 3000 Fragments arranged by size: 3000 1425 343 324 195 189 105 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2213 2576 2984 3662 4021 4180 Size: 648 1017 30 363 408 678 359 159 Cuts at: 4180 518 Size: 2042 Fragments arranged by size: 2042 1017 678 648 408 363 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2019 2074 2407 3120 3797 4945 5190 1606 Size: 413 55 333 713 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 413 333 245 55 NaeI GCC'GGC Cuts at: 2681 2681 Size: 5704 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5152 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2679 2679 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NruI TCG'CGA Cuts at: 2788 2788 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PmlI CAC'GTG Cuts at: 2311 2443 2311 Size: 132 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 5704 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 2018 2073 2322 2510 3056 830 Size: 1188 55 249 188 546 3478 Fragments arranged by size: 3478 1188 546 249 188 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2019 2074 2019 Size: 55 5649 SacI G_AGCT'C Cuts at: 2273 2273 Size: 5704 SacII CC_GC'GG Cuts at: 2408 2408 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SapI GCTCTTCn'nnn_ Cuts at: 2501 2501 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2014 2069 2318 2506 3052 4187 4868 Size: 219 969 55 249 188 546 1135 681 Cuts at: 4868 5059 826 Size: 191 1471 Fragments arranged by size: 1471 1135 969 681 546 249 219 191 188 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2223 1517 Size: 706 4998 StyI C'CwwG_G Cuts at: 513 1065 3143 513 Size: 552 2078 3074 Fragments arranged by size: 3074 2078 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3731 3748 3901 4086 4531 325 Size: 1255 2151 17 153 185 445 1498 Fragments arranged by size: 2151 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 2355 2427 Size: 274 80 33 51 1443 219 159 72 Cuts at: 2427 3950 5662 96 Size: 1523 1712 138 Fragments arranged by size: 1712 1523 1443 274 219 159 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2307 2339 5664 5696 2307 Size: 32 3325 32 2315 Fragments arranged by size: 3325 2315 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 2840 3833 2840 Size: 993 4711 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI