Restriction map for
pFN21AB8796
(Linear) (Six-base) MAPSORT of: KIBB8796.seq Check: 9935 from: 1 to: 999
KIBB8796.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 560 999
Size: 560 439
Acc65I G'GTAC_C
Cuts at: 0 545 999
Size: 545 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 999
Size: 34 965
AflIII A'CryG_T
Cuts at: 0 897 999
Size: 897 102
AlwNI CAG_nnn'CTG
Cuts at: 0 747 999
Size: 747 252
BanI G'GyrC_C
Cuts at: 0 545 656 933 967 985 999
Size: 545 111 277 34 18 14
Fragments arranged by size:
545 277 111 34 18 14
BanII G_rGCy'C
Cuts at: 0 400 999
Size: 400 599
BbeI G_GCGC'C
Cuts at: 0 660 989 999
Size: 660 329 10
Fragments arranged by size:
660 329 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 855 999
Size: 855 144
BbvCI CC'TCA_GC
Cuts at: 0 130 706 999
Size: 130 576 293
Fragments arranged by size:
576 293 130
BglI GCCn_nnn'nGGC
Cuts at: 0 140 999
Size: 140 859
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 999
Size: 285 714
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 999
Size: 316 683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 999
Size: 205 794
Bpu10I CC'TnA_GC
Cuts at: 0 130 706 999
Size: 130 576 293
Fragments arranged by size:
576 293 130
BsaAI yAC'GTr
Cuts at: 0 542 615 999
Size: 542 73 384
Fragments arranged by size:
542 384 73
BsaHI Gr'CG_yC
Cuts at: 0 657 986 999
Size: 657 329 13
Fragments arranged by size:
657 329 13
BsaWI w'CCGG_w
Cuts at: 0 767 999
Size: 767 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 887 917 999
Size: 538 30 319 30 82
Fragments arranged by size:
538 319 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 301 999
Size: 118 183 698
Fragments arranged by size:
698 183 118
BseYI C'CCAG_C
Cuts at: 0 281 288 710 937 942 999
Size: 281 7 422 227 5 57
Fragments arranged by size:
422 281 227 57 7 5
BsiHKAI G_wGCw'C
Cuts at: 0 28 857 999
Size: 28 829 142
Fragments arranged by size:
829 142 28
Bsp1286I G_dGCh'C
Cuts at: 0 28 400 857 999
Size: 28 372 457 142
Fragments arranged by size:
457 372 142 28
BspEI T'CCGG_A
Cuts at: 0 767 999
Size: 767 232
BspHI T'CATG_A
Cuts at: 0 365 999
Size: 365 634
BsrBI CCG'CTC
Cuts at: 0 641 999
Size: 641 358
BsrFI r'CCGG_y
Cuts at: 0 923 999
Size: 923 76
BstEII G'GTnAC_C
Cuts at: 0 444 999
Size: 444 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 275 693 964 999
Size: 275 418 271 35
Fragments arranged by size:
418 275 271 35
BstYI r'GATC_y
Cuts at: 0 260 620 999
Size: 260 360 379
Fragments arranged by size:
379 360 260
Bsu36I CC'TnA_GG
Cuts at: 0 9 625 999
Size: 9 616 374
Fragments arranged by size:
616 374 9
BtgI C'CryG_G
Cuts at: 0 449 999
Size: 449 550
BtsI GCAGTG_nn'
Cuts at: 0 834 999
Size: 834 165
DraIII CAC_nnn'GTG
Cuts at: 0 507 999
Size: 507 492
EarI CTCTTCn'nnn_
Cuts at: 0 580 999
Size: 580 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 205 999
Size: 34 171 794
Fragments arranged by size:
794 171 34
EcoO109I rG'GnC_Cy
Cuts at: 0 162 629 715 999
Size: 162 467 86 284
Fragments arranged by size:
467 284 162 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 696 999
Size: 696 303
HaeII r_GCGC'y
Cuts at: 0 660 989 999
Size: 660 329 10
Fragments arranged by size:
660 329 10
KasI G'GCGC_C
Cuts at: 0 656 985 999
Size: 656 329 14
Fragments arranged by size:
656 329 14
KpnI G_GTAC'C
Cuts at: 0 549 999
Size: 549 450
MslI CAynn'nnrTG
Cuts at: 0 454 691 999
Size: 454 237 308
Fragments arranged by size:
454 308 237
MspA1I CmG'CkG
Cuts at: 0 431 710 999
Size: 431 279 289
Fragments arranged by size:
431 289 279
NaeI GCC'GGC
Cuts at: 0 925 999
Size: 925 74
NarI GG'CG_CC
Cuts at: 0 657 986 999
Size: 657 329 13
Fragments arranged by size:
657 329 13
NcoI C'CATG_G
Cuts at: 0 449 999
Size: 449 550
NgoMIV G'CCGG_C
Cuts at: 0 923 999
Size: 923 76
NspI r_CATG'y
Cuts at: 0 901 999
Size: 901 98
PasI CC'CwG_GG
Cuts at: 0 591 999
Size: 591 408
PciI A'CATG_T
Cuts at: 0 897 999
Size: 897 102
PmlI CAC'GTG
Cuts at: 0 542 999
Size: 542 457
PpuMI rG'GwC_Cy
Cuts at: 0 162 629 715 999
Size: 162 467 86 284
Fragments arranged by size:
467 284 162 86
PstI C_TGCA'G
Cuts at: 0 743 932 999
Size: 743 189 67
Fragments arranged by size:
743 189 67
PvuII CAG'CTG
Cuts at: 0 710 999
Size: 710 289
SanDI GG'GwC_CC
Cuts at: 0 715 999
Size: 715 284
SexAI A'CCwGG_T
Cuts at: 0 230 999
Size: 230 769
SfcI C'TryA_G
Cuts at: 0 339 739 928 999
Size: 339 400 189 71
Fragments arranged by size:
400 339 189 71
SfoI GGC'GCC
Cuts at: 0 658 987 999
Size: 658 329 12
Fragments arranged by size:
658 329 12
StuI AGG'CCT
Cuts at: 0 76 999
Size: 76 923
StyI C'CwwG_G
Cuts at: 0 449 999
Size: 449 550
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 951 999
Size: 273 678 48
Fragments arranged by size:
678 273 48
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI BanI BanII BbeI
BbsI BbvCI BglI BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspHI BsrBI BsrFI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII EarI Eco57MI EcoO109I FalI HaeII KasI
KpnI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmlI PpuMI PstI PvuII SanDI SexAI
SfcI SfoI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaI BsaBI
BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
MfeI MluI MmeI MscI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI