(Circular) (Six-base) MAPSORT of: pFN21AB8794.seq Check: 1632 from: 1 to: 5695 pFN21AB8794 5695 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2709 2709 Size: 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2383 2400 2799 3038 1937 Size: 446 17 399 239 4594 Fragments arranged by size: 4594 446 399 239 17 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2675 3758 5142 5410 1632 Size: 1043 1083 1384 268 1917 Fragments arranged by size: 1917 1384 1083 1043 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2552 4594 5571 1203 Size: 1349 2042 977 1327 Fragments arranged by size: 2042 1349 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2162 1166 Size: 996 4699 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2313 2648 1184 Size: 1129 335 4231 Fragments arranged by size: 4231 1129 335 AlwNI CAG_nnn'CTG Cuts at: 3438 5010 3438 Size: 1572 4123 ApaI G_GGCC'C Cuts at: 2081 2094 2081 Size: 13 5682 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 3020 1870 Size: 73 1077 4545 Fragments arranged by size: 4545 1077 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2492 2697 3016 4471 Size: 325 217 627 369 336 205 319 1455 Cuts at: 4471 618 Size: 1842 Fragments arranged by size: 1842 1455 627 369 336 325 319 217 205 BanII G_rGCy'C Cuts at: 1391 2081 2094 3014 1391 Size: 690 13 920 4072 Fragments arranged by size: 4072 920 690 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2296 928 Size: 971 397 4327 Fragments arranged by size: 4327 971 397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 2877 1548 Size: 1329 4366 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2541 2750 4306 Size: 107 122 71 631 605 869 209 1556 Cuts at: 4306 136 Size: 1525 Fragments arranged by size: 1556 1525 869 631 605 209 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 2317 3112 2317 Size: 795 4900 Bme1580I G_kGCm'C Cuts at: 1790 2081 2094 2207 2224 2497 3756 4912 1790 Size: 291 13 113 17 273 1259 1156 2573 Fragments arranged by size: 2573 1259 1156 291 273 113 17 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2979 3426 4384 Size: 416 53 83 399 24 1562 447 958 Cuts at: 4384 442 Size: 1753 Fragments arranged by size: 1753 1562 958 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4355 1239 Size: 3116 2579 Bpu10I CC'TnA_GC Cuts at: 2050 2050 Size: 5695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 2080 2676 2809 4898 5394 2080 Size: 596 133 2089 496 2381 Fragments arranged by size: 2381 2089 596 496 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2037 2273 2936 1163 Size: 573 301 236 663 3922 Fragments arranged by size: 3922 663 573 301 236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2404 3145 3906 4934 1540 Size: 275 186 403 741 761 1028 2301 Fragments arranged by size: 2301 1028 761 741 403 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 3756 3841 4912 2145 Size: 1611 85 1071 2928 Fragments arranged by size: 2928 1611 1071 85 BsmI GAATG_Cn' Cuts at: 2182 3206 3299 2182 Size: 1024 93 4578 Fragments arranged by size: 4578 1024 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2094 2145 2207 2224 2497 3014 Size: 399 291 13 51 62 17 273 517 Cuts at: 3014 3756 3841 4912 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 291 273 85 62 51 17 13 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2709 3050 844 Size: 1427 438 341 3489 Fragments arranged by size: 3489 1427 438 341 BsrBI CCG'CTC Cuts at: 1394 1823 2979 3582 1394 Size: 429 1156 603 3507 Fragments arranged by size: 3507 1156 603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2361 2601 2739 4190 4364 65 Size: 1183 1113 240 138 1451 174 1396 Fragments arranged by size: 1451 1396 1183 1113 240 174 138 BsrFI r'CCGG_y Cuts at: 1700 1810 4339 4526 1700 Size: 110 2529 187 2869 Fragments arranged by size: 2869 2529 187 110 BsrGI T'GTAC_A Cuts at: 96 2418 96 Size: 2322 3373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2664 2704 2923 794 Size: 1870 40 219 3566 Fragments arranged by size: 3566 1870 219 40 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2961 3025 3392 3777 3794 5246 5332 5344 Size: 989 64 367 385 17 1452 86 12 Cuts at: 5344 5690 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 989 385 367 346 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2124 2124 Size: 5695 BtgI C'CryG_G Cuts at: 513 1065 2253 2754 513 Size: 552 1188 501 3454 Fragments arranged by size: 3454 1188 552 501 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3211 4005 4025 2111 Size: 1100 794 20 3781 Fragments arranged by size: 3781 1100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2722 2722 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2250 2388 Size: 54 1425 50 275 18 360 60 138 Cuts at: 2388 2598 2724 2751 3142 4031 5660 8 Size: 210 126 27 391 889 1629 43 Fragments arranged by size: 1629 1425 889 391 360 275 210 138 126 60 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2959 3625 1353 Size: 1606 666 3423 Fragments arranged by size: 3423 1606 666 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4282 4656 4802 387 Size: 1064 138 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2675 3758 4355 5142 5410 1239 Size: 393 1043 1083 597 787 268 1524 Fragments arranged by size: 1524 1083 1043 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2090 2090 Size: 5695 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2983 2983 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 4842 1678 Size: 3164 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2807 2839 4336 4368 4410 Size: 32 135 32 1235 32 1497 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 1235 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2401 3039 3285 669 Size: 988 281 463 638 246 3079 Fragments arranged by size: 3079 988 638 463 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2390 2600 2726 10 Size: 54 1425 343 360 198 210 126 2979 Fragments arranged by size: 2979 1425 360 343 210 198 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2198 3653 4012 4171 518 Size: 648 996 36 1455 359 159 2042 Fragments arranged by size: 2042 1455 996 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2045 2321 2695 3111 3788 4936 5181 1606 Size: 439 276 374 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 439 416 374 276 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5143 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2556 3053 4598 1132 Size: 97 1327 497 1545 2229 Fragments arranged by size: 2229 1545 1327 497 97 PasI CC'CwG_GG Cuts at: 1348 2497 1348 Size: 1149 4546 PciI A'CATG_T Cuts at: 2552 4594 2552 Size: 2042 3653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2722 1108 Size: 902 712 4081 Fragments arranged by size: 4081 902 712 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2077 2090 2077 Size: 13 5682 PstI C_TGCA'G Cuts at: 830 2637 3047 830 Size: 1807 410 3478 Fragments arranged by size: 3478 1807 410 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2045 2045 Size: 5695 SalI G'TCGA_C Cuts at: 1936 2399 3037 1936 Size: 463 638 4594 Fragments arranged by size: 4594 638 463 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2633 3043 4178 4859 5050 826 Size: 219 1588 410 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 681 410 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4700 4962 820 Size: 197 926 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2129 2274 1517 Size: 612 145 4938 Fragments arranged by size: 4938 612 145 StyI C'CwwG_G Cuts at: 513 1065 3134 513 Size: 552 2069 3074 Fragments arranged by size: 3074 2069 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2801 3722 3739 3892 4077 4522 325 Size: 1255 1221 921 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1221 921 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 3941 5653 Size: 274 80 33 51 1443 441 1523 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1523 1443 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2233 2557 2584 2752 2233 Size: 324 27 168 5176 Fragments arranged by size: 5176 324 168 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI