Restriction map for
pFN21AB8793
(Circular) (Six-base) MAPSORT of: pFN21AB8793.seq Check: 7387 from: 1 to: 5695
pFN21AB8793 5695 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2202 3038 1937
Size: 265 836 4594
Fragments arranged by size:
4594 836 265
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3758 5142 5410 1632
Size: 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 268
AfeI AGC'GCT
Cuts at: 2384 2384
Size: 5695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2324 1166
Size: 1158 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2940 1184
Size: 1756 3939
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 5663
AlwNI CAG_nnn'CTG
Cuts at: 3438 5010 3438
Size: 1572 4123
ApaI G_GGCC'C
Cuts at: 2470 2470
Size: 5695
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 2008 2628 3025 3392 2008
Size: 620 397 367 4311
Fragments arranged by size:
4311 620 397 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2963 3016 4471 618
Size: 325 217 627 1176 53 1455 1842
Fragments arranged by size:
1842 1455 1176 627 325 217 53
BanII G_rGCy'C
Cuts at: 1391 2470 2855 2914 3014 1391
Size: 1079 385 59 100 4072
Fragments arranged by size:
4072 1079 385 100 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 4525
Fragments arranged by size:
4525 971 199
BbvCI CC'TCA_GC
Cuts at: 2210 2928 2210
Size: 718 4977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4306 136
Size: 107 122 71 631 605 2634 1525
Fragments arranged by size:
2634 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 2363 2483 3112 2363
Size: 120 629 4946
Fragments arranged by size:
4946 629 120
Bme1580I G_kGCm'C
Cuts at: 1790 2470 2966 3756 4912 1790
Size: 680 496 790 1156 2573
Fragments arranged by size:
2573 1156 790 680 496
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2626 2888 4355 1239
Size: 1387 262 1467 2579
Fragments arranged by size:
2579 1467 1387 262
Bpu10I CC'TnA_GC
Cuts at: 2210 2354 2928 2210
Size: 144 574 4977
Fragments arranged by size:
4977 574 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2876 3820 4685 4983 2876
Size: 944 865 298 3588
Fragments arranged by size:
3588 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 4358 882
Size: 617 585 2274 2219
Fragments arranged by size:
2274 2219 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2693 1186
Size: 1507 4188
BseYI C'CCAG_C
Cuts at: 4898 5394 4898
Size: 496 5199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2448 3145 3906 4934 1540
Size: 275 186 447 697 761 1028 2301
Fragments arranged by size:
2301 1028 761 697 447 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2369 3756 3841 4912 2160
Size: 209 1387 85 1071 2943
Fragments arranged by size:
2943 1387 1071 209 85
BsmI GAATG_Cn'
Cuts at: 2724 3206 3299 2724
Size: 482 93 5120
Fragments arranged by size:
5120 482 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2369 2470 2855 2914 2966 3014
Size: 399 370 209 101 385 59 52 48
Cuts at: 3014 3756 3841 4912 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 370 209 101
85 59 52 48
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3050 844
Size: 2206 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2121 2922 4190 4364 65
Size: 1183 873 801 1268 174 1396
Fragments arranged by size:
1396 1268 1183 873 801 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4339 4526 1700
Size: 110 2529 187 2869
Fragments arranged by size:
2869 2529 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2008 2628 2748 3025 3392 3777 3794 5246
Size: 36 620 120 277 367 385 17 1452
Cuts at: 5246 5332 5344 5690 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 620 385 367 346 277 120
86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 2827 2873 3211 4005 4025 2562
Size: 265 46 338 794 20 4232
Fragments arranged by size:
4232 794 338 265 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 3142 4031
Size: 54 1425 50 275 18 387 925 889
Cuts at: 4031 5660 8
Size: 1629 43
Fragments arranged by size:
1629 1425 925 889 387 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3625 1353
Size: 887 1385 3423
Fragments arranged by size:
3423 1385 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2626 2888 3758 4355 5142 5410 1239
Size: 393 994 262 870 597 787 268 1524
Fragments arranged by size:
1524 994 870 787 597 393 268 262
EcoO109I rG'GnC_Cy
Cuts at: 2467 2467
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 2087
Size: 5695
FspI TGC'GCA
Cuts at: 2183 4201 2183
Size: 2018 3677
HaeII r_GCGC'y
Cuts at: 1678 2386 4842 1678
Size: 708 2456 2531
Fragments arranged by size:
2531 2456 708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 2971 3003 4336
Size: 32 135 32 944 32 423 32 1333
Cuts at: 4336 4368 4410 4442 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1333 944 423 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2036 4809 4993 1305
Size: 731 2773 184 2007
Fragments arranged by size:
2773 2007 731 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 2327 3653 4012 4171 518
Size: 648 1158 3 1326 359 159 2042
Fragments arranged by size:
2042 1326 1158 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2444 3111 3788 4936 5181 1606
Size: 838 667 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 677 667 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2182 2362 3053 4598 1132
Size: 97 953 180 691 1545 2229
Fragments arranged by size:
2229 1545 953 691 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2212 3764 3796 5277 5309 2180
Size: 32 1552 32 1481 32 2566
Fragments arranged by size:
2566 1552 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2437 1613
Size: 824 4871
PsiI TTA'TAA
Cuts at: 2255 3265 2255
Size: 1010 4685
PspOMI G'GGCC_C
Cuts at: 2466 2466
Size: 5695
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2023 2055 2023
Size: 32 5663
PstI C_TGCA'G
Cuts at: 830 2569 2880 2919 3047 830
Size: 1739 311 39 128 3478
Fragments arranged by size:
3478 1739 311 128 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2565 2876 2915 3043 4178 4859 5050
Size: 219 1520 311 39 128 1135 681 191
Cuts at: 5050 826
Size: 1471
Fragments arranged by size:
1520 1471 1135 681 311 219 191 128
39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 3799 4700 4962 820
Size: 197 926 948 908 901 262 1553
Fragments arranged by size:
1553 948 926 908 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 2182 2362 3053 1132
Size: 1050 180 691 3774
Fragments arranged by size:
3774 1050 691 180
SspI AAT'ATT
Cuts at: 5 52 2742 3619 5
Size: 47 2690 877 2081
Fragments arranged by size:
2690 2081 877 47
StuI AGG'CCT
Cuts at: 1517 2352 1517
Size: 835 4860
StyI C'CwwG_G
Cuts at: 513 1065 2004 3134 513
Size: 552 939 1130 3074
Fragments arranged by size:
3074 1130 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2039 3941 5653
Size: 274 80 33 51 1443 62 1902 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1902 1712 1443 274 138 80 62 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2491 2523 2620 2652 5655 5687 2491
Size: 32 97 32 3003 32 2499
Fragments arranged by size:
3003 2499 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 2192 3824 2192
Size: 1632 4063
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI