(Circular) (Six-base) MAPSORT of: pFN21AB8791.seq Check: 4142 from: 1 to: 5662 pFN21AB8791 5662 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3005 1937 Size: 1068 4594 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2122 3725 5109 5377 1632 Size: 490 1603 1384 268 1917 Fragments arranged by size: 1917 1603 1384 490 268 AflII C'TTAA_G Cuts at: 820 1017 2150 820 Size: 197 1133 4332 Fragments arranged by size: 4332 1133 197 AflIII A'CryG_T Cuts at: 1203 2502 2517 4561 5538 1203 Size: 1299 15 2044 977 1327 Fragments arranged by size: 2044 1327 1299 977 15 AgeI A'CCGG_T Cuts at: 2358 4493 2358 Size: 2135 3527 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2291 1166 Size: 1125 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 2755 3405 4977 2755 Size: 650 1572 3440 Fragments arranged by size: 3440 1572 650 ApaI G_GGCC'C Cuts at: 2908 2908 Size: 5662 ApaLI G'TGCA_C Cuts at: 2284 3719 4875 2284 Size: 1435 1156 3071 Fragments arranged by size: 3071 1435 1156 ApoI r'AATT_y Cuts at: 1493 2885 2971 3197 3349 5458 1493 Size: 1392 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1392 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2955 2987 1870 Size: 73 1012 32 4545 Fragments arranged by size: 4545 1012 73 32 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 4438 618 Size: 325 217 627 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 627 325 217 BanII G_rGCy'C Cuts at: 1391 2908 2981 1391 Size: 1517 73 4072 Fragments arranged by size: 4072 1517 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2539 2698 928 Size: 971 424 216 159 3892 Fragments arranged by size: 3892 971 424 216 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2156 2190 3853 3887 1903 Size: 34 219 34 1663 34 3678 Fragments arranged by size: 3678 1663 219 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 2193 1548 Size: 645 5017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2699 4273 136 Size: 107 122 71 631 605 1027 1574 1525 Fragments arranged by size: 1574 1525 1027 631 605 122 107 71 BglII A'GATC_T Cuts at: 2415 5657 2415 Size: 3242 2420 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 2288 2908 3723 4879 1790 Size: 498 620 815 1156 2573 Fragments arranged by size: 2573 1156 815 620 498 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2665 2720 3393 Size: 416 53 83 399 24 1248 55 673 Cuts at: 3393 4351 442 Size: 958 1753 Fragments arranged by size: 1753 1248 958 673 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2554 2579 2593 4322 1239 Size: 1315 25 14 1729 2579 Fragments arranged by size: 2579 1729 1315 25 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2634 2719 4325 882 Size: 617 1135 85 1606 2219 Fragments arranged by size: 2219 1606 1135 617 85 BsaAI yAC'GTr Cuts at: 493 1204 3453 493 Size: 711 2249 2702 Fragments arranged by size: 2702 2249 711 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 2400 2456 4095 4493 4767 4914 5325 2358 Size: 42 56 1639 398 274 147 411 2695 Fragments arranged by size: 2695 1639 411 398 274 147 56 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5437 Fragments arranged by size: 5437 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 1186 Size: 916 4746 BseYI C'CCAG_C Cuts at: 2629 4865 5361 2629 Size: 2236 496 2930 Fragments arranged by size: 2930 2236 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2416 2681 2803 1163 Size: 573 680 265 122 4022 Fragments arranged by size: 4022 680 573 265 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2288 3723 3808 4879 2229 Size: 59 1435 85 1071 3012 Fragments arranged by size: 3012 1435 1071 85 59 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2288 2908 2981 3723 3808 4879 Size: 399 439 59 620 73 742 85 1071 Cuts at: 4879 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 620 439 399 85 73 59 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2223 3017 844 Size: 1283 96 794 3489 Fragments arranged by size: 3489 1283 794 96 BsrBI CCG'CTC Cuts at: 1394 1823 2383 3549 1394 Size: 429 560 1166 3507 Fragments arranged by size: 3507 1166 560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 2910 4306 4493 1700 Size: 110 548 552 1396 187 2869 Fragments arranged by size: 2869 1396 552 548 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstYI r'GATC_y Cuts at: 1972 2415 2424 2992 3359 3744 3761 5213 5299 Size: 443 9 568 367 385 17 1452 86 Cuts at: 5299 5311 5657 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 568 443 385 367 346 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2131 2482 2583 513 Size: 552 1066 351 101 3592 Fragments arranged by size: 3592 1066 552 351 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2671 487 Size: 683 56 1445 3478 Fragments arranged by size: 3478 1445 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2367 3109 Size: 54 1425 50 275 18 294 243 742 Cuts at: 3109 3998 5627 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 742 294 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3592 1353 Size: 2239 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2554 2579 2593 3725 4322 5109 Size: 393 490 432 25 14 1132 597 787 Cuts at: 5109 5377 1239 Size: 268 1524 Fragments arranged by size: 1524 1132 787 597 490 432 393 268 25 14 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2255 Size: 5662 EcoO109I rG'GnC_Cy Cuts at: 2443 2904 2443 Size: 461 5201 EcoRI G'AATT_C Cuts at: 2885 2971 3349 2885 Size: 86 378 5198 Fragments arranged by size: 5198 378 86 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 5662 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 2541 4809 1678 Size: 863 2268 2531 Fragments arranged by size: 2531 2268 863 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373 Size: 32 135 32 2731 32 42 32 2626 Fragments arranged by size: 2731 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2050 2842 3006 3252 669 Size: 988 281 112 792 164 246 3079 Fragments arranged by size: 3079 988 792 281 246 164 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 2816 3261 2816 Size: 445 5217 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4776 4960 1305 Size: 3471 184 2007 Fragments arranged by size: 3471 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 2369 10 Size: 54 1425 343 294 243 3303 Fragments arranged by size: 3303 1425 343 294 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2291 2351 3620 3979 4138 518 Size: 648 1023 102 60 1269 359 159 2042 Fragments arranged by size: 2042 1269 1023 648 359 159 102 60 MspA1I CmG'CkG Cuts at: 1606 2557 2929 3078 3755 4903 5148 1606 Size: 951 372 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 372 245 149 NcoI C'CATG_G Cuts at: 513 1065 2131 2482 2583 513 Size: 552 1066 351 101 3592 Fragments arranged by size: 3592 1066 552 351 101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 2320 2506 2521 3020 4565 1132 Size: 97 1091 186 15 499 1545 2229 Fragments arranged by size: 2229 1545 1091 499 186 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5662 PciI A'CATG_T Cuts at: 2502 2517 4561 2502 Size: 15 2044 3603 Fragments arranged by size: 3603 2044 15 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5662 PfoI T'CCnGG_A Cuts at: 2609 2609 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2443 2443 Size: 5662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PspOMI G'GGCC_C Cuts at: 2904 2904 Size: 5662 PstI C_TGCA'G Cuts at: 830 2751 2940 3014 830 Size: 1921 189 74 3478 Fragments arranged by size: 3478 1921 189 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2929 2929 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 2940 3014 2940 Size: 74 5588 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2069 2473 2680 2747 2936 3010 4145 Size: 219 1024 404 207 67 189 74 1135 Cuts at: 4145 4826 5017 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1024 681 404 219 207 191 189 74 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2957 2989 1872 Size: 1085 32 4545 Fragments arranged by size: 4545 1085 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 3766 4667 4929 820 Size: 197 926 207 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 262 207 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2131 2482 2541 2583 3101 513 Size: 552 1066 351 59 42 518 3074 Fragments arranged by size: 3074 1066 552 518 351 59 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 2663 3689 3706 3859 4044 4489 Size: 1255 1067 16 1026 17 153 185 445 Cuts at: 4489 325 Size: 1498 Fragments arranged by size: 1498 1255 1067 1026 445 185 153 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3908 5620 96 Size: 274 80 33 51 1443 1931 1712 138 Fragments arranged by size: 1931 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 2900 2932 5622 5654 2387 Size: 32 481 32 2690 32 2395 Fragments arranged by size: 2690 2395 481 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2045 1316 Size: 729 4933 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2443 2590 2926 2098 Size: 345 147 336 4834 Fragments arranged by size: 4834 345 336 147 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2955 2987 1870 Size: 1085 32 4545 Fragments arranged by size: 4545 1085 32 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI