(Circular) (Six-base) MAPSORT of: pFN21AB8776.seq Check: 2287 from: 1 to: 6328 pFN21AB8776 6328 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3379 4391 5775 6043 1632 Size: 1747 1012 1384 268 1917 Fragments arranged by size: 1917 1747 1384 1012 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 3017 5159 3017 Size: 2142 4186 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 3018 1166 Size: 1852 4476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2241 1184 Size: 1057 5271 AlwNI CAG_nnn'CTG Cuts at: 3408 4071 5643 3408 Size: 663 1572 4093 Fragments arranged by size: 4093 1572 663 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 2029 2539 2841 2899 3429 3637 3863 4015 Size: 536 510 302 58 530 208 226 152 Cuts at: 4015 6124 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 536 530 510 302 226 208 152 58 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 3653 1870 Size: 73 1710 4545 Fragments arranged by size: 4545 1710 73 BamHI G'GATC_C Cuts at: 3658 4025 3658 Size: 367 5961 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3336 3649 5104 618 Size: 325 217 627 1549 313 1455 1842 Fragments arranged by size: 1842 1549 1455 627 325 313 217 BanII G_rGCy'C Cuts at: 1391 2567 3575 3647 1391 Size: 1176 1008 72 4072 Fragments arranged by size: 4072 1176 1008 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5357 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4519 4553 1903 Size: 34 2582 34 3678 Fragments arranged by size: 3678 2582 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4939 136 Size: 107 122 71 631 605 3267 1525 Fragments arranged by size: 3267 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 2818 3745 2818 Size: 927 5401 Bme1580I G_kGCm'C Cuts at: 1790 4389 5545 1790 Size: 2599 1156 2573 Fragments arranged by size: 2599 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5017 442 Size: 416 53 83 399 24 2642 958 1753 Fragments arranged by size: 2642 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2619 3123 4988 1239 Size: 947 433 504 1865 2579 Fragments arranged by size: 2579 1865 947 504 433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2164 4453 5318 5616 2164 Size: 2289 865 298 2876 Fragments arranged by size: 2876 2289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3437 4991 882 Size: 617 1938 1554 2219 Fragments arranged by size: 2219 1938 1554 617 BsaAI yAC'GTr Cuts at: 493 1204 4119 493 Size: 711 2915 2702 Fragments arranged by size: 2915 2702 711 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2681 3017 4761 5159 5433 5580 5991 2681 Size: 336 1744 398 274 147 411 3018 Fragments arranged by size: 3018 1744 411 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6103 Fragments arranged by size: 6103 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6328 BseYI C'CCAG_C Cuts at: 2327 3081 3533 5531 6027 2327 Size: 754 452 1998 496 2628 Fragments arranged by size: 2628 1998 754 496 452 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3144 1163 Size: 573 1408 4347 Fragments arranged by size: 4347 1408 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 4539 5567 1540 Size: 275 186 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2567 3575 3601 4389 4474 5545 2567 Size: 1008 26 788 85 1071 3350 Fragments arranged by size: 3350 1071 1008 788 85 26 BsmI GAATG_Cn' Cuts at: 2789 2873 3839 3932 2789 Size: 84 966 93 5185 Fragments arranged by size: 5185 966 93 84 BsmBI CGTCTCn'nnnn_ Cuts at: 2253 2253 Size: 6328 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2567 3575 3601 3647 4389 4474 5545 Size: 399 777 1008 26 46 742 85 1071 Cuts at: 5545 1391 Size: 2174 Fragments arranged by size: 2174 1071 1008 777 742 399 85 46 26 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3683 844 Size: 2839 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4823 4997 65 Size: 1183 3575 174 1396 Fragments arranged by size: 3575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3017 3537 4972 5159 1700 Size: 110 1207 520 1435 187 2869 Fragments arranged by size: 2869 1435 1207 520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 4388 5400 4388 Size: 1012 5316 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstYI r'GATC_y Cuts at: 1972 3177 3658 4025 4410 4427 5879 5965 5977 Size: 1205 481 367 385 17 1452 86 12 Cuts at: 5977 6323 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1205 481 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2400 3618 513 Size: 552 1335 1218 3223 Fragments arranged by size: 3223 1335 1218 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2553 487 Size: 683 56 1327 4262 Fragments arranged by size: 4262 1327 683 56 BtsI GCAGTG_nn' Cuts at: 3844 4638 4658 3844 Size: 794 20 5514 Fragments arranged by size: 5514 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3775 4664 6293 Size: 54 1425 50 275 18 1945 889 1629 Cuts at: 6293 8 Size: 43 Fragments arranged by size: 1945 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3775 1537 Size: 275 1963 4090 Fragments arranged by size: 4090 1963 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2656 4258 1353 Size: 1303 1602 3423 Fragments arranged by size: 3423 1602 1303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4915 5289 5435 387 Size: 1064 138 3326 374 146 1280 Fragments arranged by size: 3326 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2565 3573 2565 Size: 1008 5320 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2619 3123 3379 4391 4988 5775 Size: 393 554 433 504 256 1012 597 787 Cuts at: 5775 6043 1239 Size: 268 1524 Fragments arranged by size: 1524 1012 787 597 554 504 433 393 268 256 EcoO109I rG'GnC_Cy Cuts at: 3530 3530 Size: 6328 EcoRI G'AATT_C Cuts at: 2841 3429 3637 4015 2841 Size: 588 208 378 5154 Fragments arranged by size: 5154 588 378 208 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 5475 1678 Size: 3797 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2734 2766 3436 3468 4969 Size: 32 135 32 1162 32 670 32 1501 Cuts at: 4969 5001 5043 5075 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1501 1162 670 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3672 3918 669 Size: 988 281 1734 246 3079 Fragments arranged by size: 3079 1734 988 281 246 HindIII A'AGCT_T Cuts at: 748 2802 748 Size: 2054 4274 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5442 5626 1305 Size: 4137 184 2007 Fragments arranged by size: 4137 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4506 Fragments arranged by size: 4506 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2950 3018 3354 3454 4286 4645 4804 Size: 648 1784 68 336 100 832 359 159 Cuts at: 4804 518 Size: 2042 Fragments arranged by size: 2042 1784 832 648 359 336 159 100 68 MspA1I CmG'CkG Cuts at: 1606 3744 4421 5569 5814 1606 Size: 2138 677 1148 245 2120 Fragments arranged by size: 2138 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2400 3618 513 Size: 552 1335 1218 3223 Fragments arranged by size: 3223 1335 1218 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 3686 5231 1132 Size: 97 2457 1545 2229 Fragments arranged by size: 2457 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 3467 1108 Size: 1293 1066 3969 Fragments arranged by size: 3969 1293 1066 PfoI T'CCnGG_A Cuts at: 3060 3060 Size: 6328 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3530 3530 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PstI C_TGCA'G Cuts at: 830 3680 830 Size: 2850 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 SacI G_AGCT'C Cuts at: 2567 3575 2567 Size: 1008 5320 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SanDI GG'GwC_CC Cuts at: 3530 3530 Size: 6328 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SfcI C'TryA_G Cuts at: 826 1045 3676 4811 5492 5683 826 Size: 219 2631 1135 681 191 1471 Fragments arranged by size: 2631 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 3017 1700 Size: 1317 5011 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2143 4432 5333 5595 820 Size: 197 926 200 2289 901 262 1553 Fragments arranged by size: 2289 1553 926 901 262 200 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3686 1132 Size: 2554 3774 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6328 StyI C'CwwG_G Cuts at: 513 1065 2400 3618 3767 513 Size: 552 1335 1218 149 3074 Fragments arranged by size: 3074 1335 1218 552 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 4355 4372 4525 4710 5155 325 Size: 1255 1515 1260 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1260 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2618 3502 4574 Size: 274 80 33 51 1443 641 884 1072 Cuts at: 4574 6286 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1072 884 641 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6288 6320 6288 Size: 32 6296 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 2409 2804 2972 3140 3308 3476 4457 2409 Size: 395 168 168 168 168 981 4280 Fragments arranged by size: 4280 981 395 168 168 168 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI