Restriction map for
pFN21AB8767
(Linear) (Six-base) MAPSORT of: KIBB8767.seq Check: 3073 from: 1 to: 675
KIBB8767.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 550 675
Size: 57 493 125
Fragments arranged by size:
493 125 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 470 675
Size: 470 205
ApaI G_GGCC'C
Cuts at: 0 638 675
Size: 638 37
ApaLI G'TGCA_C
Cuts at: 0 370 675
Size: 370 305
AvaI C'yCGr_G
Cuts at: 0 442 675
Size: 442 233
BanI G'GyrC_C
Cuts at: 0 196 438 675
Size: 196 242 237
Fragments arranged by size:
242 237 196
BanII G_rGCy'C
Cuts at: 0 191 399 638 675
Size: 191 208 239 37
Fragments arranged by size:
239 208 191 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 589 675
Size: 589 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 156 190 559 593 675
Size: 156 34 369 34 82
Fragments arranged by size:
369 156 82 34 34
BlpI GC'TnA_GC
Cuts at: 0 244 675
Size: 244 431
Bme1580I G_kGCm'C
Cuts at: 0 344 374 443 638 675
Size: 344 30 69 195 37
Fragments arranged by size:
344 195 69 37 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 675
Size: 252 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 413 675
Size: 162 251 262
Fragments arranged by size:
262 251 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 346 675
Size: 49 297 329
Fragments arranged by size:
329 297 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 589 619 675
Size: 487 30 72 30 56
Fragments arranged by size:
487 72 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 420 537 675
Size: 420 117 138
Fragments arranged by size:
420 138 117
BseYI C'CCAG_C
Cuts at: 0 458 623 675
Size: 458 165 52
Fragments arranged by size:
458 165 52
BsiHKAI G_wGCw'C
Cuts at: 0 331 362 374 675
Size: 331 31 12 301
Fragments arranged by size:
331 301 31 12
Bsp1286I G_dGCh'C
Cuts at: 0 191 331 344 362 374 399 443 638
Size: 191 140 13 18 12 25 44 195
Cuts at: 638 675
Size: 37
Fragments arranged by size:
195 191 140 44 37 25 18 13
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 284 675
Size: 179 105 391
Fragments arranged by size:
391 179 105
BsrBI CCG'CTC
Cuts at: 0 131 675
Size: 131 544
BsrFI r'CCGG_y
Cuts at: 0 154 193 536 675
Size: 154 39 343 139
Fragments arranged by size:
343 154 139 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 339 675
Size: 339 336
BstEII G'GTnAC_C
Cuts at: 0 3 675
Size: 3 672
BstYI r'GATC_y
Cuts at: 0 518 675
Size: 518 157
BtsI GCAGTG_nn'
Cuts at: 0 116 155 675
Size: 116 39 520
Fragments arranged by size:
520 116 39
EaeI y'GGCC_r
Cuts at: 0 557 594 675
Size: 557 37 81
Fragments arranged by size:
557 81 37
EarI CTCTTCn'nnn_
Cuts at: 0 480 569 675
Size: 480 89 106
Fragments arranged by size:
480 106 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 162 413 550 675
Size: 57 105 251 137 125
Fragments arranged by size:
251 137 125 105 57
EcoO109I rG'GnC_Cy
Cuts at: 0 634 675
Size: 634 41
FspI TGC'GCA
Cuts at: 0 115 280 675
Size: 115 165 395
Fragments arranged by size:
395 165 115
HaeII r_GCGC'y
Cuts at: 0 231 675
Size: 231 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 675
Size: 436 32 207
Fragments arranged by size:
436 207 32
HincII GTy'rAC
Cuts at: 0 591 675
Size: 591 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 575 675
Size: 559 16 100
Fragments arranged by size:
559 100 16
MscI TGG'CCA
Cuts at: 0 559 675
Size: 559 116
MspA1I CmG'CkG
Cuts at: 0 96 627 675
Size: 96 531 48
Fragments arranged by size:
531 96 48
NaeI GCC'GGC
Cuts at: 0 195 538 675
Size: 195 343 137
Fragments arranged by size:
343 195 137
NgoMIV G'CCGG_C
Cuts at: 0 193 536 675
Size: 193 343 139
Fragments arranged by size:
343 193 139
NspI r_CATG'y
Cuts at: 0 384 675
Size: 384 291
PasI CC'CwG_GG
Cuts at: 0 469 675
Size: 469 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 469 675
Size: 99 370 206
Fragments arranged by size:
370 206 99
PfoI T'CCnGG_A
Cuts at: 0 650 675
Size: 650 25
PspOMI G'GGCC_C
Cuts at: 0 634 675
Size: 634 41
PstI C_TGCA'G
Cuts at: 0 125 314 675
Size: 125 189 361
Fragments arranged by size:
361 189 125
PvuII CAG'CTG
Cuts at: 0 96 627 675
Size: 96 531 48
Fragments arranged by size:
531 96 48
SapI GCTCTTCn'nnn_
Cuts at: 0 480 675
Size: 480 195
SfcI C'TryA_G
Cuts at: 0 121 310 675
Size: 121 189 365
Fragments arranged by size:
365 189 121
SmlI C'TyrA_G
Cuts at: 0 64 361 675
Size: 64 297 314
Fragments arranged by size:
314 297 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 637 675
Size: 22 615 38
Fragments arranged by size:
615 38 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 589 621 675
Size: 589 32 54
Fragments arranged by size:
589 54 32
Tth111I GACn'n_nGTC
Cuts at: 0 258 675
Size: 258 417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 466 675
Size: 466 209
Enzymes that do cut:
AcuI AlfI ApaI ApaLI AvaI BanI BanII BbsI
BcgI BlpI Bme1580I BmrI BpmI BpuEI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII
BstYI BtsI EaeI EarI Eco57MI EcoO109I FspI HaeII
Hin4I HincII MmeI MscI MspA1I NaeI NgoMIV NspI
PasI PflMI PfoI PspOMI PstI PvuII SapI SfcI
SmlI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI XbaI XhoI XmaI XmnI ZraI