Restriction map for
pFN21AB8759
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 376 GTAGAC GT^[AC][GT]AC
AciI 325 GCGG CCGC(-3/-1)
AclI 200 AACGTT AA^CGTT
AlwNI 511 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 213 CCTTAGG CC^T[ACGT]AGG
AvaII 336 GGACC G^G[AT]CC
BbsI 591 GTCTTC GAAGAC(2/6)
BbvI 245 GCTGC GCAGC(8/12)
BcgI 586 GCAGGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 458 CTAG C^TAG
BpuAI 591 GTCTTC GAAGAC(2/6)
BsaAI 79 TACGTA [CT]AC^GT[GA]
BsaJI 101 CCCAGG C^C[ACGT][ACGT]GG
BseRI 406 GAGGAG GAGGAG(10/8)
BsmFI 182 GGGAC GGGAC(10/14)
BspMI 510 GCAGGT ACCTGC(4/8)
BsrDI 541 CATTGC GCAATG(2/0)
BsrI 299 ACTGG ACTGG(1/-1)
Bsu36I 213 CCTTAGG CC^T[ACGT]AGG
Cac8I 516 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 336 GGACC G^G[ACGT]CC
DraII 335 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 213 CCTTAGG CC^T[ACGT]AGG
EcoNI 224 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 335 AGGACCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 245 GCTGC GC^[ACGT]GC
HaeIII 489 GGCC GG^CC
HgaI 158 GACGC GACGC(5/10)
HphI 40 GGTGA GGTGA(8/7)
ItaI 245 GCTGC GC^[ACGT]GC
MaeI 458 CTAG C^TAG
MspA1I 532 CAGCTG C[AC]G^C[GT]G
NspI 545 GCATGT [GA]CATG^[CT]
PleI 97 GACTC GAGTC(4/5)
PpuMI 335 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 200 AACGTT AA^CGTT
PstI 188 CTGCAG CTGCA^G
PvuII 532 CAGCTG CAG^CTG
Sau96I 336 GGACC G^G[ACGT]CC
SexAI 418 ACCTGGT A^CC[AT]GGT
SnaBI 79 TACGTA TAC^GTA
SpeI 457 ACTAGT A^CTAGT
TfiI 288 GAATC G^A[AT]TC
TseI 245 GCTGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 91 AAATTT [GA]^AATT[CT]
310 GAATTT
ApoI 91 AAATTT [GA]^AATT[CT]
310 GAATTT
BglII 141 AGATCT A^GATCT
176 AGATCT
BssKI 102 CCAGG ^CC[ACGT]GG
419 CCTGG
BstF5I 267 GGATG GGATG(2/0)
327 GGATG
BstNI 102 CCAGG CC^[AT]GG
419 CCTGG
BstYI 141 AGATCT [GA]^GATC[CT]
176 AGATCT
EcoRII 102 CCAGG ^CC[AT]GG
419 CCTGG
FokI 267 GGATG GGATG(9/13)
327 GGATG
HinfI 97 GACTC G^A[ACGT]TC
288 GAATC
MaeII 80 ACGT A^CGT
201 ACGT
MboII 496 GAAGA GAAGA(8/7)
592 TCTTC
MflI 141 AGATCT [GA]^GATC[CT]
176 AGATCT
MseI 22 TTAA T^TAA
133 TTAA
MvaI 102 CCAGG CC^[AT]GG
419 CCTGG
NlaIII 426 CATG CATG^
546 CATG
NlaIV 336 GGACCC GG[ACGT]^[ACGT]CC
577 GGCTCC
ScrFI 102 CCAGG CC^[ACGT]GG
419 CCTGG
TaqI 138 TCGA T^CGA
284 TCGA
Tru9I 22 TTAA T^TAA
133 TTAA
Tsp509I 92 AATT ^AATT
311 AATT
TthHB8I 138 TCGA T^CGA
284 TCGA
XhoII 141 AGATCT [GA]^GATC[CT]
176 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BpmI BsiYI BslI DdeI
DpnII MaeIII MboI MnlI NdeII
Sau3AI SfcI Tsp45I TspRI