Restriction map for
pFN21AB8751
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 367 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 197 CCCGAG C^[CT]CG[GA]G
AvaII 327 GGACC G^G[AT]CC
BbvI 216 GCAGC GCAGC(8/12)
BpmI 46 CTCCAG CTGGAG(16/14)
BsaWI 480 ACCGGA [AT]^CCGG[AT]
BseRI 507 CTCCTC GAGGAG(10/8)
BsoBI 197 CCCGAG C^[CT]CG[GA]G
BspMI 329 ACCTGC ACCTGC(4/8)
BssKI 67 CCTGG ^CC[ACGT]GG
BstNI 67 CCTGG CC^[AT]GG
BstXI 128 CCAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 240 CTCAG C^T[ACGT]AG
DraI 223 TTTAAA TTT^AAA
EarI 28 GAAGAG CTCTTC(1/4)
EcoRII 67 CCTGG ^CC[AT]GG
Fnu4HI 216 GCAGC GC^[ACGT]GC
HapII 481 CCGG C^CGG
HpaII 481 CCGG C^CGG
HphI 138 GGTGA GGTGA(8/7)
ItaI 216 GCAGC GC^[ACGT]GC
Ksp632I 28 GAAGAG CTCTTC(1/4)
MaeIII 37 GTAAC ^GT[ACGT]AC
MnlI 509 CCTC CCTC(7/6)
MslI 335 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 217 CAGCTG C[AC]G^C[GT]G
MspI 481 CCGG C^CGG
MvaI 67 CCTGG CC^[AT]GG
NlaIII 345 CATG CATG^
PstI 214 CTGCAG CTGCA^G
PvuII 217 CAGCTG CAG^CTG
ScrFI 67 CCTGG CC^[ACGT]GG
SfaNI 16 GATGC GCATC(5/9)
SfcI 214 CTGCAG C^T[GA][CT]AG
SspI 87 AATATT AAT^ATT
TfiI 353 GATTC G^A[AT]TC
TseI 216 GCAGC G^C[AT]GC
TspRI 79 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
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BalI 69 TGGCCA TGG^CCA
460 TGGCCA
BsiYI 48 CCAGATGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
481 CCGGAGTATGG
BslI 48 CCAGATGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
481 CCGGAGTATGG
BstF5I 64 CATCC GGATG(2/0)
537 GGATG
Cfr13I 327 GGACC G^G[ACGT]CC
473 GGCCC
DpnII 288 GATC ^GATC
437 GATC
FokI 64 CATCC GGATG(9/13)
537 GGATG
MboI 288 GATC ^GATC
437 GATC
MluNI 69 TGGCCA TGG^CCA
460 TGGCCA
MscI 69 TGGCCA TGG^CCA
460 TGGCCA
MseI 107 TTAA T^TAA
224 TTAA
NdeII 288 GATC ^GATC
437 GATC
Sau3AI 288 GATC ^GATC
437 GATC
Sau96I 327 GGACC G^G[ACGT]CC
473 GGCCC
Tru9I 107 TTAA T^TAA
224 TTAA
XcmI 72 CCAGTTGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
281 CCAATCTGATCGTGG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AcsI AluI ApoI BsmAI BsrI
EaeI HaeIII HinfI MboII PleI
Tsp509I