(Circular) (Six-base) MAPSORT of: pFN21AB8751.seq Check: 2047 from: 1 to: 5257 pFN21AB8751 5257 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3067 278 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2600 1937 Size: 663 4594 Acc65I G'GTAC_C Cuts at: 2578 2578 Size: 5257 AclI AA'CG_TT Cuts at: 1563 3384 3757 1563 Size: 1821 373 3063 Fragments arranged by size: 3063 1821 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3320 4704 4972 1632 Size: 1688 1384 268 1917 Fragments arranged by size: 1917 1688 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5060 AflIII A'CryG_T Cuts at: 1203 4156 5133 1203 Size: 2953 977 1327 Fragments arranged by size: 2953 1327 977 AgeI A'CCGG_T Cuts at: 4088 4088 Size: 5257 AhdI GACnn_n'nnGTC Cuts at: 3986 3986 Size: 5257 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5257 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5257 AlwNI CAG_nnn'CTG Cuts at: 2377 3000 4572 2377 Size: 623 1572 3062 Fragments arranged by size: 3062 1572 623 ApaLI G'TGCA_C Cuts at: 3314 4470 3314 Size: 1156 4101 ApoI r'AATT_y Cuts at: 1493 2147 2158 2234 2408 2566 2792 2944 5053 Size: 654 11 76 174 158 226 152 2109 Cuts at: 5053 1493 Size: 1697 Fragments arranged by size: 2109 1697 654 226 174 158 152 76 11 AseI AT'TA_AT Cuts at: 160 1032 3811 160 Size: 872 2779 1606 Fragments arranged by size: 2779 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 AvaI C'yCGr_G Cuts at: 1870 1943 2202 2582 1870 Size: 73 259 380 4545 Fragments arranged by size: 4545 380 259 73 BamHI G'GATC_C Cuts at: 2587 2954 2587 Size: 367 4890 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2578 4033 618 Size: 325 217 627 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 325 217 BanII G_rGCy'C Cuts at: 1391 2576 1391 Size: 1185 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3448 3482 1903 Size: 34 1511 34 3678 Fragments arranged by size: 3678 1511 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 4359 3149 Size: 1210 4047 BclI T'GATC_A Cuts at: 1548 1548 Size: 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3868 136 Size: 107 122 71 631 605 2196 1525 Fragments arranged by size: 2196 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5252 5252 Size: 5257 BlpI GC'TnA_GC Cuts at: 2674 2674 Size: 5257 Bme1580I G_kGCm'C Cuts at: 1790 3318 4474 1790 Size: 1528 1156 2573 Fragments arranged by size: 2573 1528 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2988 3946 442 Size: 416 53 83 399 24 1571 958 1753 Fragments arranged by size: 1753 1571 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5257 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2141 Size: 5257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 3917 1239 Size: 797 1881 2579 Fragments arranged by size: 2579 1881 797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3382 4247 4545 3382 Size: 865 298 4094 Fragments arranged by size: 4094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3920 882 Size: 617 2421 2219 Fragments arranged by size: 2421 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3048 493 Size: 711 1844 2702 Fragments arranged by size: 2702 1844 711 BsaBI GATnn'nnATC Cuts at: 5251 5251 Size: 5257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3064 3446 4081 275 Size: 53 83 186 936 1531 382 635 1451 Fragments arranged by size: 1531 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2485 3690 4088 4362 4509 4920 2485 Size: 1205 398 274 147 411 2822 Fragments arranged by size: 2822 1205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5032 Fragments arranged by size: 5032 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2503 1186 Size: 1317 3940 BseYI C'CCAG_C Cuts at: 2311 4460 4956 2311 Size: 2149 496 2612 Fragments arranged by size: 2612 2149 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4684 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2707 3468 4496 1540 Size: 275 186 706 761 1028 2301 Fragments arranged by size: 2301 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 3403 4474 3318 Size: 85 1071 4101 Fragments arranged by size: 4101 1071 85 BsmI GAATG_Cn' Cuts at: 2768 2861 2768 Size: 93 5164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2576 3318 3403 4474 1391 Size: 399 786 742 85 1071 2174 Fragments arranged by size: 2174 1071 786 742 399 85 BspEI T'CCGG_A Cuts at: 4920 4920 Size: 5257 BspHI T'CATG_A Cuts at: 3146 4876 3146 Size: 1730 3527 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 2612 844 Size: 1499 269 3489 Fragments arranged by size: 3489 1499 269 BsrBI CCG'CTC Cuts at: 1394 1823 3144 1394 Size: 429 1321 3507 Fragments arranged by size: 3507 1321 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3752 3926 65 Size: 1183 2504 174 1396 Fragments arranged by size: 2504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3901 4088 1700 Size: 110 2091 187 2869 Fragments arranged by size: 2869 2091 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5257 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5257 BssSI C'ACGA_G Cuts at: 3317 4329 3317 Size: 1012 4245 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5257 BstBI TT'CG_AA Cuts at: 4061 4061 Size: 5257 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5257 BstXI CCAn_nnnn'nTGG Cuts at: 2140 2140 Size: 5257 BstYI r'GATC_y Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252 Size: 615 367 385 17 1452 86 12 346 Cuts at: 5252 1972 Size: 1977 Fragments arranged by size: 1977 1452 615 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4705 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4518 Fragments arranged by size: 4518 683 56 BtsI GCAGTG_nn' Cuts at: 2090 2773 3567 3587 2090 Size: 683 794 20 3760 Fragments arranged by size: 3760 794 683 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5222 DraI TTT'AAA Cuts at: 2230 2561 2230 Size: 331 4926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4264 809 Size: 507 2948 1802 Fragments arranged by size: 2948 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2152 2465 Size: 54 1425 50 275 18 244 78 313 Cuts at: 2465 2704 3593 5222 8 Size: 239 889 1629 43 Fragments arranged by size: 1629 1425 889 313 275 244 239 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2704 1537 Size: 275 892 4090 Fragments arranged by size: 4090 892 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 3187 1353 Size: 675 1159 3423 Fragments arranged by size: 3423 1159 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3844 4218 4364 387 Size: 1064 138 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 3320 3917 4704 4972 1239 Size: 393 404 1284 597 787 268 1524 Fragments arranged by size: 1524 1284 787 597 404 393 268 EcoRI G'AATT_C Cuts at: 2566 2944 2566 Size: 378 4879 FspI TGC'GCA Cuts at: 3763 3763 Size: 5257 HaeII r_GCGC'y Cuts at: 1678 4404 1678 Size: 2726 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 3898 3930 3972 Size: 32 135 32 812 32 1482 32 42 Cuts at: 3972 4004 1373 Size: 32 2626 Fragments arranged by size: 2626 1482 812 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 2847 669 Size: 988 281 663 246 3079 Fragments arranged by size: 3079 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5257 HpaI GTT'AAC Cuts at: 2847 2847 Size: 5257 KpnI G_GTAC'C Cuts at: 2582 2582 Size: 5257 MfeI C'AATT_G Cuts at: 2856 2856 Size: 5257 MluI A'CGCG_T Cuts at: 5133 5133 Size: 5257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 2555 4371 4555 1305 Size: 882 368 1816 184 2007 Fragments arranged by size: 2007 1816 882 368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2076 2467 10 Size: 54 1425 343 244 391 2800 Fragments arranged by size: 2800 1425 391 343 244 54 MslI CAynn'nnrTG Cuts at: 518 1166 2344 3215 3574 3733 518 Size: 648 1178 871 359 159 2042 Fragments arranged by size: 2042 1178 871 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 2673 3350 4498 4743 1606 Size: 618 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 618 449 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4705 NdeI CA'TA_TG Cuts at: 387 387 Size: 5257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5257 NotI GC'GGCC_GC Cuts at: 2704 2704 Size: 5257 NspI r_CATG'y Cuts at: 1132 1229 2615 4160 1132 Size: 97 1386 1545 2229 Fragments arranged by size: 2229 1545 1386 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5257 PciI A'CATG_T Cuts at: 4156 4156 Size: 5257 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5257 PmeI GTTT'AAAC Cuts at: 2561 2561 Size: 5257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3326 3358 4839 4871 3326 Size: 32 1481 32 3712 Fragments arranged by size: 3712 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5257 PsiI TTA'TAA Cuts at: 2827 2827 Size: 5257 PstI C_TGCA'G Cuts at: 830 2223 2609 830 Size: 1393 386 3478 Fragments arranged by size: 3478 1393 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5257 PvuII CAG'CTG Cuts at: 2224 2224 Size: 5257 SalI G'TCGA_C Cuts at: 1936 2599 1936 Size: 663 4594 SbfI CC_TGCA'GG Cuts at: 2609 2609 Size: 5257 ScaI AGT'ACT Cuts at: 3505 5217 3505 Size: 1712 3545 SfcI C'TryA_G Cuts at: 826 1045 2219 2605 3740 4421 4612 826 Size: 219 1174 386 1135 681 191 1471 Fragments arranged by size: 1471 1174 1135 681 386 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5257 SmaI CCC'GGG Cuts at: 1872 2584 1872 Size: 712 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3361 4262 4524 820 Size: 197 926 1418 901 262 1553 Fragments arranged by size: 1553 1418 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3048 493 Size: 2555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5257 SphI G_CATG'C Cuts at: 1132 2615 1132 Size: 1483 3774 SspI AAT'ATT Cuts at: 5 52 2094 3181 5 Size: 47 2042 1087 2081 Fragments arranged by size: 2081 2042 1087 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5257 StyI C'CwwG_G Cuts at: 513 1065 2696 513 Size: 552 1631 3074 Fragments arranged by size: 3074 1631 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 3301 3454 3639 4084 325 Size: 1255 1704 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3503 5215 96 Size: 274 80 33 51 1443 1526 1712 138 Fragments arranged by size: 1712 1526 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5217 5249 5217 Size: 32 5225 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5257 XbaI T'CTAG_A Cuts at: 2593 2593 Size: 5257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2084 2293 2084 Size: 209 5048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5257 XmaI C'CCGG_G Cuts at: 1870 2582 1870 Size: 712 4545 XmnI GAAnn'nnTTC Cuts at: 3386 3386 Size: 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3065 276 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI