(Circular) (Six-base) MAPSORT of: pFN21AB8742.seq Check: 7651 from: 1 to: 5830 pFN21AB8742 5830 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3893 5277 5545 1632 Size: 2261 1384 268 1917 Fragments arranged by size: 2261 1917 1384 268 AfeI AGC'GCT Cuts at: 2878 2878 Size: 5830 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 2087 4559 2087 Size: 2472 3358 AleI CACnn'nnGTG Cuts at: 1166 2225 1166 Size: 1059 4771 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2669 1184 Size: 1485 4345 AlwNI CAG_nnn'CTG Cuts at: 2550 3041 3573 5145 2550 Size: 491 532 1572 3235 Fragments arranged by size: 3235 1572 532 491 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2711 3139 3365 3517 5626 1493 Size: 1218 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 428 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3056 3155 1870 Size: 73 1113 99 4545 Fragments arranged by size: 4545 1113 99 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2304 2741 2792 3149 1391 Size: 679 234 437 51 357 4072 Fragments arranged by size: 4072 679 437 357 234 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2475 5825 2475 Size: 3350 2480 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2109 3891 5047 1790 Size: 319 1782 1156 2573 Fragments arranged by size: 2573 1782 1156 319 BmgBI CAC'GTC Cuts at: 1318 2665 1318 Size: 1347 4483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2358 2932 3561 Size: 416 53 83 399 24 941 574 629 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 958 941 629 574 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4490 1239 Size: 3251 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 3955 4820 5118 2054 Size: 1901 865 298 2766 Fragments arranged by size: 2766 1901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2525 2793 4493 882 Size: 617 1026 268 1700 2219 Fragments arranged by size: 2219 1700 1026 617 268 BsaAI yAC'GTr Cuts at: 493 1204 2719 3621 493 Size: 711 1515 902 2702 Fragments arranged by size: 2702 1515 902 711 BsaBI GATnn'nnATC Cuts at: 2474 2480 5824 2474 Size: 6 3344 2480 Fragments arranged by size: 3344 2480 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2486 4263 4661 4935 5082 5493 2486 Size: 1777 398 274 147 411 2823 Fragments arranged by size: 2823 1777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 2816 2846 1178 Size: 30 165 30 1305 30 78 30 4162 Fragments arranged by size: 4162 1305 165 78 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 2716 2719 2840 3074 1186 Size: 1495 35 3 121 234 3942 Fragments arranged by size: 3942 1495 234 121 35 3 BseYI C'CCAG_C Cuts at: 2057 2063 5033 5529 2057 Size: 6 2970 496 2358 Fragments arranged by size: 2970 2358 496 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2574 3054 1163 Size: 573 838 480 3939 Fragments arranged by size: 3939 838 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3891 3976 5047 3891 Size: 85 1071 4674 Fragments arranged by size: 4674 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 BsmBI CGTCTCn'nnnn_ Cuts at: 2777 2777 Size: 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2109 2304 2741 2792 3149 3891 Size: 399 280 39 195 437 51 357 742 Cuts at: 3891 3976 5047 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 399 357 280 195 85 51 39 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 2328 3719 5449 2328 Size: 1391 1730 2709 Fragments arranged by size: 2709 1730 1391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 3185 844 Size: 1319 1022 3489 Fragments arranged by size: 3489 1319 1022 BsrBI CCG'CTC Cuts at: 1394 1823 2683 3717 1394 Size: 429 860 1034 3507 Fragments arranged by size: 3507 1034 860 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3130 4325 4499 65 Size: 1183 1882 1195 174 1396 Fragments arranged by size: 1882 1396 1195 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2642 2642 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2475 3160 3527 3912 3929 5381 5467 5479 Size: 503 685 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 685 503 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2251 487 Size: 683 56 1025 4066 Fragments arranged by size: 4066 1025 683 56 BtsI GCAGTG_nn' Cuts at: 2052 3346 4140 4160 2052 Size: 1294 794 20 3722 Fragments arranged by size: 3722 1294 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2147 2182 529 Size: 35 1583 35 4177 Fragments arranged by size: 4177 1583 35 35 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2499 3277 4166 Size: 54 1425 50 275 18 669 778 889 Cuts at: 4166 5795 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 778 669 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 3760 1353 Size: 1256 1151 3423 Fragments arranged by size: 3423 1256 1151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3893 4490 5277 5545 1239 Size: 393 2261 597 787 268 1524 Fragments arranged by size: 2261 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2602 2638 2602 Size: 36 5794 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2880 4977 1678 Size: 1202 2097 2531 Fragments arranged by size: 2531 2097 1202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 2187 2219 2714 Size: 32 135 32 501 32 82 32 495 Cuts at: 2714 2746 4471 4503 4545 4577 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 501 495 135 82 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 10 Size: 54 1425 343 669 3339 Fragments arranged by size: 3339 1425 669 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2225 2333 3788 4147 4306 518 Size: 648 858 201 108 1455 359 159 2042 Fragments arranged by size: 2042 1455 858 648 359 201 159 108 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2029 3188 4733 1132 Size: 97 800 1159 1545 2229 Fragments arranged by size: 2229 1545 1159 800 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4162 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PmlI CAC'GTG Cuts at: 2719 2719 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2663 1613 Size: 1050 4780 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspXI vC'TCGA_Gb Cuts at: 3056 3056 Size: 5830 PstI C_TGCA'G Cuts at: 830 2658 3182 830 Size: 1828 524 3478 Fragments arranged by size: 3478 1828 524 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2609 2609 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SexAI A'CCwGG_T Cuts at: 2234 2234 Size: 5830 SfcI C'TryA_G Cuts at: 826 1045 2654 3178 4313 4994 5185 826 Size: 219 1609 524 1135 681 191 1471 Fragments arranged by size: 1609 1471 1135 681 524 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 3056 3934 4835 5097 820 Size: 197 926 126 987 878 901 262 1553 Fragments arranged by size: 1553 987 926 901 878 262 197 126 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 2670 3269 513 Size: 552 939 666 599 3074 Fragments arranged by size: 3074 939 666 599 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3066 4076 5788 Size: 274 80 33 51 1443 1089 1010 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1712 1443 1089 1010 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 2958 2990 5790 5822 2708 Size: 32 218 32 2800 32 2716 Fragments arranged by size: 2800 2716 218 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2384 2437 2038 Size: 346 53 5431 Fragments arranged by size: 5431 346 53 XhoI C'TCGA_G Cuts at: 1943 3056 1943 Size: 1113 4717 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3000 3959 3000 Size: 959 4871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI