(Circular) (Six-base) MAPSORT of: pFN21AB8739.seq Check: 5596 from: 1 to: 4861 pFN21AB8739 4861 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2671 278 Size: 53 83 186 936 1135 2468 Fragments arranged by size: 2468 1135 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2204 1937 Size: 267 4594 Acc65I G'GTAC_C Cuts at: 2182 2182 Size: 4861 AclI AA'CG_TT Cuts at: 1563 2988 3361 1563 Size: 1425 373 3063 Fragments arranged by size: 3063 1425 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 4308 4576 1632 Size: 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4664 AflIII A'CryG_T Cuts at: 1203 3760 4737 1203 Size: 2557 977 1327 Fragments arranged by size: 2557 1327 977 AgeI A'CCGG_T Cuts at: 3692 3692 Size: 4861 AhdI GACnn_n'nnGTC Cuts at: 3590 3590 Size: 4861 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4861 AlwNI CAG_nnn'CTG Cuts at: 2604 4176 2604 Size: 1572 3289 ApaLI G'TGCA_C Cuts at: 2918 4074 2918 Size: 1156 3705 ApoI r'AATT_y Cuts at: 1493 2170 2396 2548 4657 1493 Size: 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 677 226 152 AseI AT'TA_AT Cuts at: 160 1032 3415 160 Size: 872 2383 1606 Fragments arranged by size: 2383 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4861 AvaI C'yCGr_G Cuts at: 1870 1943 2186 1870 Size: 73 243 4545 Fragments arranged by size: 4545 243 73 BamHI G'GATC_C Cuts at: 2191 2558 2191 Size: 367 4494 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 3637 618 Size: 325 217 627 395 1455 1842 Fragments arranged by size: 1842 1455 627 395 325 217 BanII G_rGCy'C Cuts at: 1391 2180 1391 Size: 789 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3890 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3052 3086 1903 Size: 34 1115 34 3678 Fragments arranged by size: 3678 1115 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2753 3963 2753 Size: 1210 3651 BclI T'GATC_A Cuts at: 1548 1548 Size: 4861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3472 136 Size: 107 122 71 631 605 1800 1525 Fragments arranged by size: 1800 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4856 4856 Size: 4861 BlpI GC'TnA_GC Cuts at: 2032 2278 2032 Size: 246 4615 Bme1580I G_kGCm'C Cuts at: 1790 2922 4078 1790 Size: 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 BmgBI CAC'GTC Cuts at: 1318 2078 1318 Size: 760 4101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2592 3550 442 Size: 416 53 83 399 24 1175 958 1753 Fragments arranged by size: 1753 1175 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3521 1239 Size: 2282 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2986 3851 4149 2986 Size: 865 298 3698 Fragments arranged by size: 3698 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3524 882 Size: 617 2025 2219 Fragments arranged by size: 2219 2025 617 BsaAI yAC'GTr Cuts at: 493 1204 2652 493 Size: 711 1448 2702 Fragments arranged by size: 2702 1448 711 BsaBI GATnn'nnATC Cuts at: 2141 4855 2141 Size: 2714 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2668 3050 3685 275 Size: 53 83 186 936 1135 382 635 1451 Fragments arranged by size: 1451 1135 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3294 3692 3966 4113 4524 3294 Size: 398 274 147 411 3631 Fragments arranged by size: 3631 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4636 Fragments arranged by size: 4636 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4861 BseYI C'CCAG_C Cuts at: 4064 4560 4064 Size: 496 4365 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2311 3072 4100 1540 Size: 275 186 310 761 1028 2301 Fragments arranged by size: 2301 1028 761 310 275 186 BsiHKAI G_wGCw'C Cuts at: 2922 3007 4078 2922 Size: 85 1071 3705 Fragments arranged by size: 3705 1071 85 BsmI GAATG_Cn' Cuts at: 2372 2465 2372 Size: 93 4768 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2922 3007 4078 1391 Size: 399 390 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 85 BspEI T'CCGG_A Cuts at: 4524 4524 Size: 4861 BspHI T'CATG_A Cuts at: 2750 4480 2750 Size: 1730 3131 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2216 844 Size: 1372 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2748 1394 Size: 429 925 3507 Fragments arranged by size: 3507 925 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3356 3530 65 Size: 1183 2108 174 1396 Fragments arranged by size: 2108 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3505 3692 1700 Size: 110 1695 187 2869 Fragments arranged by size: 2869 1695 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4861 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4861 BssSI C'ACGA_G Cuts at: 2921 3933 2921 Size: 1012 3849 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4861 BstBI TT'CG_AA Cuts at: 3665 3665 Size: 4861 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4861 BstYI r'GATC_y Cuts at: 1972 2191 2558 2943 2960 4412 4498 4510 4856 Size: 219 367 385 17 1452 86 12 346 Cuts at: 4856 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3370 Fragments arranged by size: 3370 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4122 Fragments arranged by size: 4122 683 56 BtsI GCAGTG_nn' Cuts at: 2377 3171 3191 2377 Size: 794 20 4047 Fragments arranged by size: 4047 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4861 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4826 DraI TTT'AAA Cuts at: 2165 2165 Size: 4861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3868 809 Size: 507 2552 1802 Fragments arranged by size: 2552 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2308 3197 4826 Size: 54 1425 50 275 18 478 889 1629 Cuts at: 4826 8 Size: 43 Fragments arranged by size: 1629 1425 889 478 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2308 1537 Size: 275 496 4090 Fragments arranged by size: 4090 496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2038 2791 1353 Size: 685 753 3423 Fragments arranged by size: 3423 753 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3448 3822 3968 387 Size: 1064 138 1859 374 146 1280 Fragments arranged by size: 1859 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 3521 4308 4576 1239 Size: 393 1292 597 787 268 1524 Fragments arranged by size: 1524 1292 787 597 393 268 EcoRI G'AATT_C Cuts at: 2170 2548 2170 Size: 378 4483 FspI TGC'GCA Cuts at: 3367 3367 Size: 4861 HaeII r_GCGC'y Cuts at: 1678 4008 1678 Size: 2330 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3502 3534 3576 3608 1373 Size: 32 135 32 1930 32 42 32 2626 Fragments arranged by size: 2626 1930 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2205 2451 669 Size: 988 281 267 246 3079 Fragments arranged by size: 3079 988 281 267 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4861 HpaI GTT'AAC Cuts at: 2451 2451 Size: 4861 KpnI G_GTAC'C Cuts at: 2186 2186 Size: 4861 MfeI C'AATT_G Cuts at: 2460 2460 Size: 4861 MluI A'CGCG_T Cuts at: 4737 4737 Size: 4861 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3975 4159 1305 Size: 2670 184 2007 Fragments arranged by size: 2670 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3039 Fragments arranged by size: 3039 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2150 2819 3178 3337 518 Size: 648 894 90 669 359 159 2042 Fragments arranged by size: 2042 894 669 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2277 2954 4102 4347 1606 Size: 671 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 671 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3370 Fragments arranged by size: 3370 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4861 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4861 NotI GC'GGCC_GC Cuts at: 2308 2308 Size: 4861 NspI r_CATG'y Cuts at: 1132 1229 2219 3764 1132 Size: 97 990 1545 2229 Fragments arranged by size: 2229 1545 990 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4861 PciI A'CATG_T Cuts at: 3760 3760 Size: 4861 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4861 PmeI GTTT'AAAC Cuts at: 2165 2165 Size: 4861 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 2962 4443 4475 2930 Size: 32 1481 32 3316 Fragments arranged by size: 3316 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4861 PsiI TTA'TAA Cuts at: 2431 2431 Size: 4861 PstI C_TGCA'G Cuts at: 830 2213 830 Size: 1383 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4861 SalI G'TCGA_C Cuts at: 1936 2203 1936 Size: 267 4594 SapI GCTCTTCn'nnn_ Cuts at: 2038 2038 Size: 4861 SbfI CC_TGCA'GG Cuts at: 2213 2213 Size: 4861 ScaI AGT'ACT Cuts at: 3109 4821 3109 Size: 1712 3149 SfcI C'TryA_G Cuts at: 826 1045 2087 2209 3344 4025 4216 826 Size: 219 1042 122 1135 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4861 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4861 SmaI CCC'GGG Cuts at: 1872 2188 1872 Size: 316 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2965 3866 4128 820 Size: 197 926 1022 901 262 1553 Fragments arranged by size: 1553 1022 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2652 493 Size: 2159 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4861 SphI G_CATG'C Cuts at: 1132 2219 1132 Size: 1087 3774 SspI AAT'ATT Cuts at: 5 52 2785 5 Size: 47 2733 2081 Fragments arranged by size: 2733 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4861 StyI C'CwwG_G Cuts at: 513 1065 2004 2300 513 Size: 552 939 296 3074 Fragments arranged by size: 3074 939 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 2905 3058 3243 3688 325 Size: 1255 1308 17 153 185 445 1498 Fragments arranged by size: 1498 1308 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3107 4819 96 Size: 274 80 33 51 1443 1130 1712 138 Fragments arranged by size: 1712 1443 1130 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4821 4853 4821 Size: 32 4829 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4861 XbaI T'CTAG_A Cuts at: 2197 2197 Size: 4861 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4861 XmaI C'CCGG_G Cuts at: 1870 2186 1870 Size: 316 4545 XmnI GAAnn'nnTTC Cuts at: 2990 2990 Size: 4861 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2669 276 Size: 53 83 186 936 1135 2468 Fragments arranged by size: 2468 1135 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI