(Circular) (Six-base) MAPSORT of: pFN21AB8728.seq Check: 3839 from: 1 to: 5821 pFN21AB8728 5821 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2507 2967 3164 1937 Size: 570 460 197 4594 Fragments arranged by size: 4594 570 460 197 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 3884 5268 5536 1632 Size: 1010 1242 1384 268 1917 Fragments arranged by size: 1917 1384 1242 1010 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 2474 1166 Size: 1308 4513 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3102 1184 Size: 1918 3903 AlwNI CAG_nnn'CTG Cuts at: 2145 2503 2758 3564 5136 2145 Size: 358 255 806 1572 2830 Fragments arranged by size: 2830 1572 806 358 255 ApaLI G'TGCA_C Cuts at: 2477 3878 5034 2477 Size: 1401 1156 3264 Fragments arranged by size: 3264 1401 1156 ApoI r'AATT_y Cuts at: 1493 2791 3130 3356 3508 5617 1493 Size: 1298 339 226 152 2109 1697 Fragments arranged by size: 2109 1697 1298 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 3142 4597 618 Size: 325 217 627 479 876 1455 1842 Fragments arranged by size: 1842 1455 876 627 479 325 217 BanII G_rGCy'C Cuts at: 1391 2025 3140 1391 Size: 634 1115 4072 Fragments arranged by size: 4072 1115 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 2630 2717 3045 928 Size: 971 207 524 87 328 3704 Fragments arranged by size: 3704 971 524 328 207 87 BbvCI CC'TCA_GC Cuts at: 2886 2886 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2077 2808 5816 2077 Size: 731 3008 2082 Fragments arranged by size: 3008 2082 731 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2271 2403 2481 3882 5038 1790 Size: 481 132 78 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 481 132 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2387 2651 2919 4481 1239 Size: 1106 42 264 268 1562 2579 Fragments arranged by size: 2579 1562 1106 268 264 42 Bpu10I CC'TnA_GC Cuts at: 2886 2886 Size: 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2935 3612 493 Size: 711 1731 677 2702 Fragments arranged by size: 2702 1731 711 677 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2637 3628 4010 4645 Size: 53 83 186 936 1104 991 382 635 Cuts at: 4645 275 Size: 1451 Fragments arranged by size: 1451 1104 991 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2951 2973 4254 4652 4926 5073 5484 2951 Size: 22 1281 398 274 147 411 3288 Fragments arranged by size: 3288 1281 411 398 274 147 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2305 2903 1186 Size: 1119 598 4104 Fragments arranged by size: 4104 1119 598 BseYI C'CCAG_C Cuts at: 5024 5520 5024 Size: 496 5325 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 1163 Size: 573 891 4357 Fragments arranged by size: 4357 891 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 3882 3967 5038 2481 Size: 1401 85 1071 3264 Fragments arranged by size: 3264 1401 1071 85 BsmI GAATG_Cn' Cuts at: 2983 3332 3425 2983 Size: 349 93 5379 Fragments arranged by size: 5379 349 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2271 2403 2481 3140 3882 3967 Size: 399 235 246 132 78 659 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 659 399 246 235 132 85 78 BspEI T'CCGG_A Cuts at: 2973 5484 2973 Size: 2511 3310 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2201 3176 844 Size: 1357 975 3489 Fragments arranged by size: 3489 1357 975 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2343 4465 4652 1700 Size: 110 533 2122 187 2869 Fragments arranged by size: 2869 2122 533 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2077 2808 2853 3151 3518 3903 3920 5372 Size: 105 731 45 298 367 385 17 1452 Cuts at: 5372 5458 5470 5816 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 731 385 367 346 298 105 86 45 17 12 BstZ17I GTA'TAC Cuts at: 2508 2508 Size: 5821 Bsu36I CC'TnA_GG Cuts at: 2027 2027 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 2550 2716 513 Size: 552 1485 166 3618 Fragments arranged by size: 3618 1485 552 166 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2361 2644 3337 4131 4151 2361 Size: 283 693 794 20 4031 Fragments arranged by size: 4031 794 693 283 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2485 2485 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786 Size: 54 1425 50 275 18 1438 889 1629 Cuts at: 5786 8 Size: 43 Fragments arranged by size: 1629 1438 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2249 3751 1353 Size: 755 141 1502 3423 Fragments arranged by size: 3423 1502 755 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2387 2642 2651 2919 3884 4481 Size: 393 713 42 255 9 268 965 597 Cuts at: 4481 5268 5536 1239 Size: 787 268 1524 Fragments arranged by size: 1524 965 787 713 597 393 268 268 255 42 9 EcoNI CCTnn'n_nnAGG Cuts at: 2456 2456 Size: 5821 EcoO109I rG'GnC_Cy Cuts at: 2142 2500 2142 Size: 358 5463 EcoRI G'AATT_C Cuts at: 2791 3130 3508 2791 Size: 339 378 5104 Fragments arranged by size: 5104 378 339 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2319 2545 2319 Size: 226 5595 FspI TGC'GCA Cuts at: 2931 4327 2931 Size: 1396 4425 HaeII r_GCGC'y Cuts at: 1678 4968 1678 Size: 3290 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 2769 2801 2879 Size: 32 135 32 1022 32 143 32 78 Cuts at: 2879 2911 4462 4494 4536 4568 1373 Size: 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 1022 143 135 78 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2542 3165 3411 669 Size: 988 281 604 623 246 3079 Fragments arranged by size: 3079 988 623 604 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 4935 5119 1305 Size: 1132 2498 184 2007 Fragments arranged by size: 2498 2007 1132 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 2961 3110 3779 4138 4297 518 Size: 648 1308 487 149 669 359 159 2042 Fragments arranged by size: 2042 1308 669 648 487 359 159 149 MspA1I CmG'CkG Cuts at: 1606 3237 3914 5062 5307 1606 Size: 1631 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 245 NaeI GCC'GGC Cuts at: 2345 2345 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 2550 513 Size: 552 1485 3784 Fragments arranged by size: 3784 1485 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2343 2343 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2578 3179 4724 1132 Size: 97 1349 601 1545 2229 Fragments arranged by size: 2229 1545 1349 601 97 PasI CC'CwG_GG Cuts at: 1348 2139 2271 2661 1348 Size: 791 132 390 4508 Fragments arranged by size: 4508 791 390 132 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2145 2839 1108 Size: 1037 694 4090 Fragments arranged by size: 4090 1037 694 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PmlI CAC'GTG Cuts at: 2935 2935 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2952 1613 Size: 1339 4482 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 2388 2499 3173 830 Size: 1558 111 674 3478 Fragments arranged by size: 3478 1558 674 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2384 2495 3169 4304 4985 5176 826 Size: 219 1339 111 674 1135 681 191 1471 Fragments arranged by size: 1471 1339 1135 681 674 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 2578 3179 1132 Size: 1446 601 3774 Fragments arranged by size: 3774 1446 601 SspI AAT'ATT Cuts at: 5 52 3019 3745 5 Size: 47 2967 726 2081 Fragments arranged by size: 2967 2081 726 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2550 3260 513 Size: 552 1485 710 3074 Fragments arranged by size: 3074 1485 710 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 4067 5779 Size: 274 80 33 51 1443 846 1244 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1443 1244 846 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 3022 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI