(Circular) (Six-base) MAPSORT of: pFN21AB8725.seq Check: 1468 from: 1 to: 6697 pFN21AB8725 6697 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4507 278 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 4040 1937 Size: 496 1607 4594 Fragments arranged by size: 4594 1607 496 Acc65I G'GTAC_C Cuts at: 3071 4018 3071 Size: 947 5750 AclI AA'CG_TT Cuts at: 1563 4824 5197 1563 Size: 3261 373 3063 Fragments arranged by size: 3261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 3033 3793 4760 6144 6412 1632 Size: 636 765 760 967 1384 268 1917 Fragments arranged by size: 1917 1384 967 765 760 636 268 AfeI AGC'GCT Cuts at: 2903 2903 Size: 6697 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6500 AflIII A'CryG_T Cuts at: 1203 5596 6573 1203 Size: 4393 977 1327 Fragments arranged by size: 4393 1327 977 AgeI A'CCGG_T Cuts at: 5528 5528 Size: 6697 AhdI GACnn_n'nnGTC Cuts at: 5426 5426 Size: 6697 AleI CACnn'nnGTG Cuts at: 1166 3626 1166 Size: 2460 4237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3867 1184 Size: 2683 4014 AlwNI CAG_nnn'CTG Cuts at: 3552 4440 6012 3552 Size: 888 1572 4237 Fragments arranged by size: 4237 1572 888 ApaLI G'TGCA_C Cuts at: 2878 3575 3852 4754 5910 2878 Size: 697 277 902 1156 3665 Fragments arranged by size: 3665 1156 902 697 277 ApoI r'AATT_y Cuts at: 1493 4006 4232 4384 6493 1493 Size: 2513 226 152 2109 1697 Fragments arranged by size: 2513 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5251 160 Size: 872 4219 1606 Fragments arranged by size: 4219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 AvaI C'yCGr_G Cuts at: 1870 1943 2494 4022 1870 Size: 73 551 1528 4545 Fragments arranged by size: 4545 1528 551 73 AvrII C'CTAG_G Cuts at: 3475 3475 Size: 6697 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3063 3096 3063 Size: 33 6664 BamHI G'GATC_C Cuts at: 2424 4027 4394 2424 Size: 1603 367 4727 Fragments arranged by size: 4727 1603 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3071 3325 4018 5473 618 Size: 325 217 627 1284 254 693 1455 1842 Fragments arranged by size: 1842 1455 1284 693 627 325 254 217 BanII G_rGCy'C Cuts at: 1391 2027 2070 3123 3182 3270 3546 4016 1391 Size: 636 43 1053 59 88 276 470 4072 Fragments arranged by size: 4072 1053 636 470 276 88 59 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3406 928 Size: 971 1507 4219 Fragments arranged by size: 4219 1507 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3298 3332 4888 4922 1903 Size: 34 1361 34 1556 34 3678 Fragments arranged by size: 3678 1556 1361 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4589 5799 4589 Size: 1210 5487 BclI T'GATC_A Cuts at: 1548 2631 3003 1548 Size: 1083 372 5242 Fragments arranged by size: 5242 1083 372 BfrBI ATG'CAT Cuts at: 3986 3986 Size: 6697 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5308 136 Size: 107 122 71 631 605 3636 1525 Fragments arranged by size: 3636 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2475 6692 2475 Size: 4217 2480 BlpI GC'TnA_GC Cuts at: 4114 4114 Size: 6697 Bme1580I G_kGCm'C Cuts at: 1790 2882 3330 3579 3856 4758 5914 1790 Size: 1092 448 249 277 902 1156 2573 Fragments arranged by size: 2573 1156 1092 902 448 277 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6697 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2283 2670 4428 Size: 416 53 83 399 24 866 387 1758 Cuts at: 4428 5386 442 Size: 958 1753 Fragments arranged by size: 1758 1753 958 866 416 399 387 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6697 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2828 2240 Size: 588 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 5357 1239 Size: 802 3316 2579 Fragments arranged by size: 3316 2579 802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4822 5687 5985 4822 Size: 865 298 5534 Fragments arranged by size: 5534 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2814 3755 5360 882 Size: 617 552 763 941 1605 2219 Fragments arranged by size: 2219 1605 941 763 617 552 BsaAI yAC'GTr Cuts at: 493 1204 2932 3500 4488 493 Size: 711 1728 568 988 2702 Fragments arranged by size: 2702 1728 988 711 568 BsaBI GATnn'nnATC Cuts at: 2855 3308 3666 6691 2855 Size: 453 358 3025 2861 Fragments arranged by size: 3025 2861 453 358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 4504 4886 5521 Size: 53 83 186 936 1161 1810 382 635 Cuts at: 5521 275 Size: 1451 Fragments arranged by size: 1810 1451 1161 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3658 5130 5528 5802 5949 6360 3658 Size: 1472 398 274 147 411 3995 Fragments arranged by size: 3995 1472 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6472 Fragments arranged by size: 6472 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3632 3722 3749 3890 1186 Size: 2446 90 27 141 3993 Fragments arranged by size: 3993 2446 141 90 27 BseYI C'CCAG_C Cuts at: 2338 2372 3545 5900 6396 2338 Size: 34 1173 2355 496 2639 Fragments arranged by size: 2639 2355 1173 496 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2918 3356 1163 Size: 573 1182 438 4504 Fragments arranged by size: 4504 1182 573 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4147 4908 5936 1540 Size: 275 186 2146 761 1028 2301 Fragments arranged by size: 2301 2146 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2882 3123 3182 3270 3579 3856 4758 4843 Size: 812 241 59 88 309 277 902 85 Cuts at: 4843 5914 2070 Size: 1071 2853 Fragments arranged by size: 2853 1071 902 812 309 277 241 88 85 59 BsmI GAATG_Cn' Cuts at: 2320 3012 4208 4301 2320 Size: 692 1196 93 4716 Fragments arranged by size: 4716 1196 692 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2070 2882 3123 3182 3270 3330 Size: 399 237 43 812 241 59 88 60 Cuts at: 3330 3546 3579 3856 4016 4758 4843 5914 1391 Size: 216 33 277 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 812 742 399 277 241 237 216 160 88 85 60 59 43 33 BspEI T'CCGG_A Cuts at: 3658 6360 3658 Size: 2702 3995 BspHI T'CATG_A Cuts at: 3174 3270 4586 6316 3174 Size: 96 1316 1730 3555 Fragments arranged by size: 3555 1730 1316 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4052 844 Size: 3208 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4584 1394 Size: 429 2761 3507 Fragments arranged by size: 3507 2761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 3402 5192 5366 65 Size: 1183 835 1319 1790 174 1396 Fragments arranged by size: 1790 1396 1319 1183 835 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5341 5528 1700 Size: 110 3531 187 2869 Fragments arranged by size: 3531 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3018 96 Size: 2922 3775 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6697 BssSI C'ACGA_G Cuts at: 2063 4757 5769 2063 Size: 2694 1012 2991 Fragments arranged by size: 2991 2694 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2567 3992 794 Size: 1773 1425 3499 Fragments arranged by size: 3499 1773 1425 BstBI TT'CG_AA Cuts at: 5501 5501 Size: 6697 BstEII G'GTnAC_C Cuts at: 1184 2041 3961 1184 Size: 857 1920 3920 Fragments arranged by size: 3920 1920 857 BstXI CCAn_nnnn'nTGG Cuts at: 3391 3391 Size: 6697 BstYI r'GATC_y Cuts at: 1972 2424 2475 2806 4027 4394 4779 4796 6248 Size: 452 51 331 1221 367 385 17 1452 Cuts at: 6248 6334 6346 6692 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1221 452 385 367 346 331 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2399 2521 3756 2399 Size: 122 1235 5340 Fragments arranged by size: 5340 1235 122 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2828 3222 3966 513 Size: 552 939 33 791 394 744 3244 Fragments arranged by size: 3244 939 791 744 552 394 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2275 3079 3301 487 Size: 683 56 1049 804 222 3883 Fragments arranged by size: 3883 1049 804 683 222 56 BtsI GCAGTG_nn' Cuts at: 2565 4213 5007 5027 2565 Size: 1648 794 20 4235 Fragments arranged by size: 4235 1648 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3093 3128 529 Size: 35 2529 35 4098 Fragments arranged by size: 4098 2529 35 35 DraI TTT'AAA Cuts at: 4001 4001 Size: 6697 DraIII CAC_nnn'GTG Cuts at: 2257 2680 3350 2257 Size: 423 670 5604 Fragments arranged by size: 5604 670 423 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5704 809 Size: 507 4388 1802 Fragments arranged by size: 4388 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2207 2248 Size: 54 1425 50 275 18 294 83 41 Cuts at: 2248 2265 4144 5033 6662 8 Size: 17 1879 889 1629 43 Fragments arranged by size: 1879 1629 1425 889 294 275 83 54 50 43 41 18 17 EagI C'GGCC_G Cuts at: 1537 1812 4144 1537 Size: 275 2332 4090 Fragments arranged by size: 4090 2332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3221 3365 3431 3608 3726 3867 4627 1353 Size: 1868 144 66 177 118 141 760 3423 Fragments arranged by size: 3423 1868 760 177 144 141 118 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5284 5658 5804 387 Size: 1064 138 3695 374 146 1280 Fragments arranged by size: 3695 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 3121 3180 3268 2068 Size: 1053 59 88 5497 Fragments arranged by size: 5497 1053 88 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2268 3033 3793 4760 5357 6144 Size: 393 409 227 765 760 967 597 787 Cuts at: 6144 6412 1239 Size: 268 1524 Fragments arranged by size: 1524 967 787 765 760 597 409 393 268 227 EcoO109I rG'GnC_Cy Cuts at: 2590 3752 2590 Size: 1162 5535 EcoRI G'AATT_C Cuts at: 4006 4384 4006 Size: 378 6319 EcoRV GAT'ATC Cuts at: 2089 2089 Size: 6697 FspI TGC'GCA Cuts at: 3695 5203 3695 Size: 1508 5189 HaeII r_GCGC'y Cuts at: 1678 2905 3207 5844 1678 Size: 1227 302 2637 2531 Fragments arranged by size: 2637 2531 1227 302 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 5338 5370 5412 Size: 32 135 32 905 32 2829 32 42 Cuts at: 5412 5444 1373 Size: 32 2626 Fragments arranged by size: 2829 2626 905 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4041 4287 669 Size: 988 281 2103 246 3079 Fragments arranged by size: 3079 2103 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6697 HpaI GTT'AAC Cuts at: 4287 4287 Size: 6697 KpnI G_GTAC'C Cuts at: 3075 4022 3075 Size: 947 5750 MfeI C'AATT_G Cuts at: 4296 4296 Size: 6697 MluI A'CGCG_T Cuts at: 6573 6573 Size: 6697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2149 2750 3128 3665 5811 5995 1305 Size: 805 39 601 378 537 2146 184 2007 Fragments arranged by size: 2146 2007 805 601 537 378 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 10 Size: 54 1425 343 418 4457 Fragments arranged by size: 4457 1425 418 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 2987 2990 3626 3890 3959 4655 Size: 648 1473 348 3 636 264 69 696 Cuts at: 4655 5014 5173 518 Size: 359 159 2042 Fragments arranged by size: 2042 1473 696 648 636 359 348 264 159 69 3 MspA1I CmG'CkG Cuts at: 1606 2039 2422 4113 4790 5938 6183 1606 Size: 433 383 1691 677 1148 245 2120 Fragments arranged by size: 2120 1691 1148 677 433 383 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2828 3222 513 Size: 552 939 824 394 3988 Fragments arranged by size: 3988 939 824 552 394 NdeI CA'TA_TG Cuts at: 387 387 Size: 6697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6697 NotI GC'GGCC_GC Cuts at: 4144 4144 Size: 6697 NsiI A_TGCA'T Cuts at: 3988 3988 Size: 6697 NspI r_CATG'y Cuts at: 1132 1229 4055 5600 1132 Size: 97 2826 1545 2229 Fragments arranged by size: 2826 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6697 PciI A'CATG_T Cuts at: 5596 5596 Size: 6697 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 2834 1108 Size: 1149 577 4971 Fragments arranged by size: 4971 1149 577 PmeI GTTT'AAAC Cuts at: 4001 4001 Size: 6697 PmlI CAC'GTG Cuts at: 2932 2932 Size: 6697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4798 6279 6311 4766 Size: 32 1481 32 5152 Fragments arranged by size: 5152 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6697 PsiI TTA'TAA Cuts at: 4267 4267 Size: 6697 PstI C_TGCA'G Cuts at: 830 4049 830 Size: 3219 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6697 SacI G_AGCT'C Cuts at: 2070 3123 3182 3270 2070 Size: 1053 59 88 5497 Fragments arranged by size: 5497 1053 88 59 SacII CC_GC'GG Cuts at: 2040 2040 Size: 6697 SalI G'TCGA_C Cuts at: 1936 4039 1936 Size: 2103 4594 SbfI CC_TGCA'GG Cuts at: 4049 4049 Size: 6697 ScaI AGT'ACT Cuts at: 4945 6657 4945 Size: 1712 4985 SfcI C'TryA_G Cuts at: 826 1045 4045 5180 5861 6052 826 Size: 219 3000 1135 681 191 1471 Fragments arranged by size: 3000 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6697 SmaI CCC'GGG Cuts at: 1872 4024 1872 Size: 2152 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4801 5702 5964 820 Size: 197 926 2858 901 262 1553 Fragments arranged by size: 2858 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4488 493 Size: 3995 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6697 SphI G_CATG'C Cuts at: 1132 4055 1132 Size: 2923 3774 SspI AAT'ATT Cuts at: 5 52 3065 4621 5 Size: 47 3013 1556 2081 Fragments arranged by size: 3013 2081 1556 47 StuI AGG'CCT Cuts at: 1517 2526 3144 1517 Size: 1009 618 5070 Fragments arranged by size: 5070 1009 618 StyI C'CwwG_G Cuts at: 513 1065 2004 2777 2828 3075 3222 3237 3475 Size: 552 939 773 51 247 147 15 238 Cuts at: 3475 4136 513 Size: 661 3074 Fragments arranged by size: 3074 939 773 661 552 247 238 147 51 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3313 3415 3871 4724 4741 4894 5079 Size: 1255 1733 102 456 853 17 153 185 Cuts at: 5079 5524 325 Size: 445 1498 Fragments arranged by size: 1733 1498 1255 853 456 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2709 3018 3585 Size: 274 80 33 51 1443 732 309 567 Cuts at: 3585 4943 6655 96 Size: 1358 1712 138 Fragments arranged by size: 1712 1443 1358 732 567 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6657 6689 6657 Size: 32 6665 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6697 XbaI T'CTAG_A Cuts at: 4033 4033 Size: 6697 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2983 3459 2983 Size: 476 6221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6697 XmaI C'CCGG_G Cuts at: 1870 4022 1870 Size: 2152 4545 XmnI GAAnn'nnTTC Cuts at: 4826 4826 Size: 6697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4505 276 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI