(Circular) (Six-base) MAPSORT of: pFN21AB8722.seq Check: 3012 from: 1 to: 6265 pFN21AB8722 6265 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 2379 3608 1937 Size: 217 225 1229 4594 Fragments arranged by size: 4594 1229 225 217 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3538 4328 5712 5980 1632 Size: 1906 790 1384 268 1917 Fragments arranged by size: 1917 1906 1384 790 268 AfeI AGC'GCT Cuts at: 2746 2746 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 2213 820 Size: 197 1196 4872 Fragments arranged by size: 4872 1196 197 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 2260 4994 2260 Size: 2734 3531 AleI CACnn'nnGTG Cuts at: 1166 3299 1166 Size: 2133 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 3561 4008 5580 3561 Size: 447 1572 4246 Fragments arranged by size: 4246 1572 447 ApaI G_GGCC'C Cuts at: 3123 3422 3123 Size: 299 5966 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2101 3574 3800 3952 6061 1493 Size: 608 1473 226 152 2109 1697 Fragments arranged by size: 2109 1697 1473 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2616 3590 1870 Size: 73 673 974 4545 Fragments arranged by size: 4545 974 673 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2957 3586 5041 618 Size: 325 217 627 1170 629 1455 1842 Fragments arranged by size: 1842 1455 1170 629 627 325 217 BanII G_rGCy'C Cuts at: 1391 2451 2637 3123 3422 3495 3584 1391 Size: 1060 186 486 299 73 89 4072 Fragments arranged by size: 4072 1060 486 299 186 89 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3359 928 Size: 971 1460 3834 Fragments arranged by size: 3834 1460 971 BbvCI CC'TCA_GC Cuts at: 3101 3101 Size: 6265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2390 4876 136 Size: 107 122 71 631 605 718 2486 1525 Fragments arranged by size: 2486 1525 718 631 605 122 107 71 BglII A'GATC_T Cuts at: 2086 6260 2086 Size: 4174 2091 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 3123 3422 4326 5482 1790 Size: 1333 299 904 1156 2573 Fragments arranged by size: 2573 1333 1156 904 299 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2153 2309 3049 Size: 416 53 83 399 24 736 156 740 Cuts at: 3049 3528 3996 4954 442 Size: 479 468 958 1753 Fragments arranged by size: 1753 958 740 736 479 468 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2903 4925 1239 Size: 1223 441 2022 2579 Fragments arranged by size: 2579 2022 1223 441 Bpu10I CC'TnA_GC Cuts at: 3101 3101 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3347 4390 5255 5553 3347 Size: 1043 865 298 4059 Fragments arranged by size: 4059 1043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2606 2615 2771 3141 3371 4928 882 Size: 617 1107 9 156 370 230 1557 2219 Fragments arranged by size: 2219 1557 1107 617 370 230 156 9 BsaAI yAC'GTr Cuts at: 493 1204 2848 4056 493 Size: 711 1644 1208 2702 Fragments arranged by size: 2702 1644 1208 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2979 4698 5096 5370 5517 5928 2979 Size: 1719 398 274 147 411 3316 Fragments arranged by size: 3316 1719 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3542 3572 1178 Size: 30 165 30 2139 30 3871 Fragments arranged by size: 3871 2139 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3290 3335 1186 Size: 2104 45 4116 Fragments arranged by size: 4116 2104 45 BseYI C'CCAG_C Cuts at: 2234 3460 5468 5964 2234 Size: 1226 2008 496 2535 Fragments arranged by size: 2535 2008 1226 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2149 2840 2873 1163 Size: 573 413 691 33 4555 Fragments arranged by size: 4555 691 573 413 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2370 3715 4476 5504 1540 Size: 275 186 369 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 369 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 4326 4411 5482 2637 Size: 1689 85 1071 3420 Fragments arranged by size: 3420 1689 1071 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2637 3123 3422 3495 3584 4326 Size: 399 661 186 486 299 73 89 742 Cuts at: 4326 4411 5482 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 486 399 299 186 89 85 73 BspEI T'CCGG_A Cuts at: 2979 5928 2979 Size: 2949 3316 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 2921 3620 844 Size: 1598 479 699 3489 Fragments arranged by size: 3489 1598 699 479 BsrBI CCG'CTC Cuts at: 1394 1823 3349 4152 1394 Size: 429 1526 803 3507 Fragments arranged by size: 3507 1526 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2245 4760 4934 65 Size: 1183 997 2515 174 1396 Fragments arranged by size: 2515 1396 1183 997 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2369 4909 5096 1700 Size: 110 559 2540 187 2869 Fragments arranged by size: 2869 2540 559 187 110 BsrGI T'GTAC_A Cuts at: 96 2037 96 Size: 1941 4324 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2494 2767 2494 Size: 273 5992 BstYI r'GATC_y Cuts at: 1972 2086 2972 3279 3595 3962 4347 4364 5816 Size: 114 886 307 316 367 385 17 1452 Cuts at: 5816 5902 5914 6260 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 886 385 367 346 316 307 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3270 3270 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 2004 2207 3091 3231 513 Size: 552 939 203 884 140 3547 Fragments arranged by size: 3547 939 884 552 203 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2780 487 Size: 683 56 1554 3972 Fragments arranged by size: 3972 1554 683 56 BtsI GCAGTG_nn' Cuts at: 2125 2308 2400 2890 3781 4575 4595 2125 Size: 183 92 490 891 794 20 3795 Fragments arranged by size: 3795 891 794 490 183 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 2353 2353 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3108 3712 4601 Size: 54 1425 50 275 18 1278 604 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1278 889 604 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 3383 3483 4195 1353 Size: 1203 827 100 712 3423 Fragments arranged by size: 3423 1203 827 712 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3422 4852 5226 5372 387 Size: 1064 138 1833 1430 374 146 1280 Fragments arranged by size: 1833 1430 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2903 3538 4328 4925 5712 5980 Size: 393 830 441 635 790 597 787 268 Cuts at: 5980 1239 Size: 1524 Fragments arranged by size: 1524 830 790 787 635 597 441 393 268 EcoO109I rG'GnC_Cy Cuts at: 2262 2431 2482 2578 2683 3418 3419 2262 Size: 169 51 96 105 735 1 5108 Fragments arranged by size: 5108 735 169 105 96 51 1 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 6265 FspI TGC'GCA Cuts at: 3342 4771 3342 Size: 1429 4836 HaeII r_GCGC'y Cuts at: 1678 2748 5412 1678 Size: 1070 2664 2531 Fragments arranged by size: 2664 2531 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 3542 3574 4906 Size: 32 135 32 674 32 1264 32 1332 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 1264 674 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3110 10 Size: 54 1425 343 1278 3165 Fragments arranged by size: 3165 1425 1278 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2067 2765 3299 4223 4582 4741 518 Size: 648 901 698 534 924 359 159 2042 Fragments arranged by size: 2042 924 901 698 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2795 3093 3681 4358 5506 5751 1606 Size: 1189 298 588 677 1148 245 2120 Fragments arranged by size: 2120 1189 1148 677 588 298 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2207 3231 513 Size: 552 939 203 1024 3547 Fragments arranged by size: 3547 1024 939 552 203 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 3442 1108 Size: 1245 1089 3931 Fragments arranged by size: 3931 1245 1089 PfoI T'CCnGG_A Cuts at: 2427 2478 2772 2427 Size: 51 294 5920 Fragments arranged by size: 5920 294 51 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2262 2431 2482 2578 2262 Size: 169 51 96 5949 Fragments arranged by size: 5949 169 96 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 3119 3418 3119 Size: 299 5966 PstI C_TGCA'G Cuts at: 830 2132 2145 2631 3617 830 Size: 1302 13 486 986 3478 Fragments arranged by size: 3478 1302 986 486 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2795 2795 Size: 6265 SacI G_AGCT'C Cuts at: 2637 2637 Size: 6265 SacII CC_GC'GG Cuts at: 3094 3094 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 3383 3483 3383 Size: 100 6165 SbfI CC_TGCA'GG Cuts at: 2631 3617 2631 Size: 986 5279 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2128 2141 2627 3297 3613 4748 5429 Size: 219 1083 13 486 670 316 1135 681 Cuts at: 5429 5620 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1083 681 670 486 316 219 191 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 3362 4369 5270 5532 820 Size: 197 926 270 1149 1007 901 262 1553 Fragments arranged by size: 1553 1149 1007 926 901 270 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2004 2207 2995 3231 3704 513 Size: 552 939 203 788 236 473 3074 Fragments arranged by size: 3074 939 788 552 473 236 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 2456 4292 4309 4462 4647 5092 Size: 1255 780 96 1836 17 153 185 445 Cuts at: 5092 325 Size: 1498 Fragments arranged by size: 1836 1498 1255 780 445 185 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2037 3450 4511 Size: 274 80 33 51 1443 60 1413 1061 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1413 1061 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3238 3238 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 3050 4394 3050 Size: 1344 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI