(Circular) (Six-base) MAPSORT of: pFN21AB8720.seq Check: 3584 from: 1 to: 6865 pFN21AB8720 6865 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4675 278 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4208 1937 Size: 2271 4594 Acc65I G'GTAC_C Cuts at: 4186 4186 Size: 6865 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2903 4928 6312 6580 1632 Size: 714 557 2025 1384 268 1917 Fragments arranged by size: 2025 1917 1384 714 557 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6668 AflIII A'CryG_T Cuts at: 1203 3982 5764 6741 1203 Size: 2779 1782 977 1327 Fragments arranged by size: 2779 1782 1327 977 AgeI A'CCGG_T Cuts at: 4069 5696 4069 Size: 1627 5238 AhdI GACnn_n'nnGTC Cuts at: 5594 5594 Size: 6865 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6865 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6865 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4130 4162 4130 Size: 32 6833 AlwNI CAG_nnn'CTG Cuts at: 3113 3238 3923 4608 6180 3113 Size: 125 685 685 1572 3798 Fragments arranged by size: 3798 1572 685 685 125 ApaI G_GGCC'C Cuts at: 2475 2475 Size: 6865 ApaLI G'TGCA_C Cuts at: 2974 3436 4922 6078 2974 Size: 462 1486 1156 3761 Fragments arranged by size: 3761 1486 1156 462 ApoI r'AATT_y Cuts at: 1493 2051 3011 3316 3360 4174 4400 4552 6661 Size: 558 960 305 44 814 226 152 2109 Cuts at: 6661 1493 Size: 1697 Fragments arranged by size: 2109 1697 960 814 558 305 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 5419 160 Size: 872 4387 1606 Fragments arranged by size: 4387 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 2547 2639 2661 2800 2941 3831 4190 Size: 73 604 92 22 139 141 890 359 Cuts at: 4190 1870 Size: 4545 Fragments arranged by size: 4545 890 604 359 141 139 92 73 22 BamHI G'GATC_C Cuts at: 4195 4562 4195 Size: 367 6498 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2528 2551 3181 4186 5641 Size: 325 217 627 741 23 630 1005 1455 Cuts at: 5641 618 Size: 1842 Fragments arranged by size: 1842 1455 1005 741 630 627 325 217 23 BanII G_rGCy'C Cuts at: 1391 2222 2475 3611 3651 4100 4121 4184 1391 Size: 831 253 1136 40 449 21 63 4072 Fragments arranged by size: 4072 1136 831 449 253 63 40 21 BbeI G_GCGC'C Cuts at: 2555 2555 Size: 6865 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 2067 2098 2334 2894 928 Size: 971 132 36 31 236 560 4899 Fragments arranged by size: 4899 971 560 236 132 36 31 BbvCI CC'TCA_GC Cuts at: 3190 3190 Size: 6865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5056 5090 1903 Size: 34 3119 34 3678 Fragments arranged by size: 3678 3119 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4757 5967 4757 Size: 1210 5655 BclI T'GATC_A Cuts at: 1548 1548 Size: 6865 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2838 5476 136 Size: 107 122 71 631 605 1166 2638 1525 Fragments arranged by size: 2638 1525 1166 631 605 122 107 71 BglII A'GATC_T Cuts at: 6860 6860 Size: 6865 BlpI GC'TnA_GC Cuts at: 3512 4115 4282 3512 Size: 603 167 6095 Fragments arranged by size: 6095 603 167 Bme1580I G_kGCm'C Cuts at: 1790 2475 2533 2978 3421 3440 4926 6082 1790 Size: 685 58 445 443 19 1486 1156 2573 Fragments arranged by size: 2573 1486 1156 685 445 443 58 19 BmgBI CAC'GTC Cuts at: 1318 2424 3441 1318 Size: 1106 1017 4742 Fragments arranged by size: 4742 1106 1017 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4596 5554 442 Size: 416 53 83 399 24 3179 958 1753 Fragments arranged by size: 3179 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4119 4119 Size: 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 3229 3765 3900 4020 5525 1239 Size: 997 993 536 135 120 1505 2579 Fragments arranged by size: 2579 1505 997 993 536 135 120 Bpu10I CC'TnA_GC Cuts at: 3190 4094 3190 Size: 904 5961 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4142 4990 5855 6153 4142 Size: 848 865 298 4854 Fragments arranged by size: 4854 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5528 882 Size: 617 4029 2219 Fragments arranged by size: 4029 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3985 4656 493 Size: 711 2781 671 2702 Fragments arranged by size: 2781 2702 711 671 BsaBI GATnn'nnATC Cuts at: 6859 6859 Size: 6865 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2552 4672 5054 5689 Size: 53 83 186 936 1019 2120 382 635 Cuts at: 5689 275 Size: 1451 Fragments arranged by size: 2120 1451 1019 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2167 3217 4069 5298 5696 5970 6117 6528 2167 Size: 1050 852 1229 398 274 147 411 2504 Fragments arranged by size: 2504 1229 1050 852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4018 4048 4082 4112 1178 Size: 30 165 30 2615 30 34 30 3931 Fragments arranged by size: 3931 2615 165 34 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2494 2929 3735 4145 1186 Size: 1308 435 806 410 3906 Fragments arranged by size: 3906 1308 806 435 410 BseYI C'CCAG_C Cuts at: 2467 2519 2752 2983 6068 6564 2467 Size: 52 233 231 3085 496 2768 Fragments arranged by size: 3085 2768 496 233 231 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6292 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2823 3428 4315 5076 6104 1540 Size: 275 186 822 605 887 761 1028 2301 Fragments arranged by size: 2301 1028 887 822 761 605 275 186 BsiHKAI G_wGCw'C Cuts at: 2978 3440 4100 4926 5011 6082 2978 Size: 462 660 826 85 1071 3761 Fragments arranged by size: 3761 1071 826 660 462 85 BsmI GAATG_Cn' Cuts at: 4376 4469 4376 Size: 93 6772 BsmBI CGTCTCn'nnnn_ Cuts at: 2430 3628 2430 Size: 1198 5667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2222 2475 2533 2978 3421 3440 3611 Size: 399 432 253 58 445 443 19 171 Cuts at: 3611 3651 4100 4121 4184 4926 5011 6082 1391 Size: 40 449 21 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 445 443 432 399 253 171 85 63 58 40 21 19 BspEI T'CCGG_A Cuts at: 3217 6528 3217 Size: 3311 3554 BspHI T'CATG_A Cuts at: 4023 4754 6484 4023 Size: 731 1730 4404 Fragments arranged by size: 4404 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2803 4220 844 Size: 1959 1417 3489 Fragments arranged by size: 3489 1959 1417 BsrBI CCG'CTC Cuts at: 1394 1823 2666 2958 4752 1394 Size: 429 843 292 1794 3507 Fragments arranged by size: 3507 1794 843 429 292 BsrDI GCAATG_nn' Cuts at: 65 1248 3672 5360 5534 65 Size: 1183 2424 1688 174 1396 Fragments arranged by size: 2424 1688 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2818 3552 4069 5509 5696 1700 Size: 110 1008 734 517 1440 187 2869 Fragments arranged by size: 2869 1440 1008 734 517 187 110 BsrGI T'GTAC_A Cuts at: 96 2250 96 Size: 2154 4711 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 4925 5937 4925 Size: 1012 5853 BstAPI GCAn_nnn'nTGC Cuts at: 794 2969 794 Size: 2175 4690 BstBI TT'CG_AA Cuts at: 5669 5669 Size: 6865 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6865 BstXI CCAn_nnnn'nTGG Cuts at: 3604 3718 3604 Size: 114 6751 BstYI r'GATC_y Cuts at: 1972 3995 4195 4562 4947 4964 6416 6502 6514 Size: 2023 200 367 385 17 1452 86 12 Cuts at: 6514 6860 1972 Size: 346 1977 Fragments arranged by size: 2023 1977 1452 385 367 346 200 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3762 4122 3762 Size: 360 6505 BtgI C'CryG_G Cuts at: 513 1065 2004 3603 513 Size: 552 939 1599 3775 Fragments arranged by size: 3775 1599 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6126 Fragments arranged by size: 6126 683 56 BtsI GCAGTG_nn' Cuts at: 2598 2994 4381 5175 5195 2598 Size: 396 1387 794 20 4268 Fragments arranged by size: 4268 1387 794 396 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3832 3867 529 Size: 35 3268 35 3527 Fragments arranged by size: 3527 3268 35 35 DraI TTT'AAA Cuts at: 3358 4169 3358 Size: 811 6054 DraIII CAC_nnn'GTG Cuts at: 3524 3524 Size: 6865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5872 809 Size: 507 4556 1802 Fragments arranged by size: 4556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2226 2820 Size: 54 1425 50 275 18 210 186 594 Cuts at: 2820 3092 3229 3425 4056 4312 5201 6830 8 Size: 272 137 196 631 256 889 1629 43 Fragments arranged by size: 1629 1425 889 631 594 275 272 256 210 196 186 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2820 3425 4312 1537 Size: 275 1008 605 887 4090 Fragments arranged by size: 4090 1008 887 605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3882 4795 1353 Size: 2529 913 3423 Fragments arranged by size: 3423 2529 913 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5452 5826 5972 387 Size: 1064 138 3863 374 146 1280 Fragments arranged by size: 3863 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4098 4098 Size: 6865 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2346 2903 3229 3765 3900 4020 Size: 393 604 110 557 326 536 135 120 Cuts at: 4020 4928 5525 6312 6580 1239 Size: 908 597 787 268 1524 Fragments arranged by size: 1524 908 787 604 597 557 536 393 326 268 135 120 110 EcoO109I rG'GnC_Cy Cuts at: 2471 2472 2558 2945 3077 3110 2471 Size: 1 86 387 132 33 6226 Fragments arranged by size: 6226 387 132 86 33 1 EcoRI G'AATT_C Cuts at: 4174 4552 4174 Size: 378 6487 EcoRV GAT'ATC Cuts at: 3889 3889 Size: 6865 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2048 2048 Size: 6865 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 2555 3559 6012 1678 Size: 877 1004 2453 2531 Fragments arranged by size: 2531 2453 1004 877 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 4018 4050 4082 Size: 32 135 32 1178 32 1236 32 32 Cuts at: 4082 4114 5506 5538 5580 5612 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 1236 1178 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 3407 748 Size: 2659 4206 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KasI G'GCGC_C Cuts at: 2551 2551 Size: 6865 KpnI G_GTAC'C Cuts at: 4190 4190 Size: 6865 MfeI C'AATT_G Cuts at: 3272 4464 3272 Size: 1192 5673 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5979 6163 1305 Size: 4674 184 2007 Fragments arranged by size: 4674 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4058 10 Size: 54 1425 343 2226 2817 Fragments arranged by size: 2817 2226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2775 4823 5182 5341 518 Size: 648 1609 2048 359 159 2042 Fragments arranged by size: 2048 2042 1609 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3167 3424 4114 4281 4958 6106 6351 Size: 1150 411 257 690 167 677 1148 245 Cuts at: 6351 1606 Size: 2120 Fragments arranged by size: 2120 1150 1148 690 677 411 257 245 167 NaeI GCC'GGC Cuts at: 2820 3554 2820 Size: 734 6131 NarI GG'CG_CC Cuts at: 2552 2552 Size: 6865 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5374 Fragments arranged by size: 5374 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6865 NgoMIV G'CCGG_C Cuts at: 2818 3552 2818 Size: 734 6131 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6865 NotI GC'GGCC_GC Cuts at: 3425 4312 3425 Size: 887 5978 NspI r_CATG'y Cuts at: 1132 1229 4223 5768 1132 Size: 97 2994 1545 2229 Fragments arranged by size: 2994 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2586 2693 3597 1348 Size: 1238 107 904 4616 Fragments arranged by size: 4616 1238 904 107 PciI A'CATG_T Cuts at: 5764 5764 Size: 6865 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 1108 Size: 1239 5626 PfoI T'CCnGG_A Cuts at: 2738 2738 Size: 6865 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PmlI CAC'GTG Cuts at: 3985 3985 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4934 4966 6447 6479 4934 Size: 32 1481 32 5320 Fragments arranged by size: 5320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3077 3077 Size: 6865 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6865 PsiI TTA'TAA Cuts at: 4435 4435 Size: 6865 PspOMI G'GGCC_C Cuts at: 2471 2471 Size: 6865 PspXI vC'TCGA_Gb Cuts at: 2661 2661 Size: 6865 PstI C_TGCA'G Cuts at: 830 3109 4217 830 Size: 2279 1108 3478 Fragments arranged by size: 3478 2279 1108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 2756 4114 2756 Size: 1358 5507 SacI G_AGCT'C Cuts at: 4100 4100 Size: 6865 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SanDI GG'GwC_CC Cuts at: 3077 3077 Size: 6865 SapI GCTCTTCn'nnn_ Cuts at: 3882 3882 Size: 6865 SbfI CC_TGCA'GG Cuts at: 3109 4217 3109 Size: 1108 5757 ScaI AGT'ACT Cuts at: 3814 5113 6825 3814 Size: 1299 1712 3854 Fragments arranged by size: 3854 1712 1299 SfcI C'TryA_G Cuts at: 826 1045 3105 3990 4213 5348 6029 6220 826 Size: 219 2060 885 223 1135 681 191 1471 Fragments arranged by size: 2060 1471 1135 885 681 223 219 191 SfoI GGC'GCC Cuts at: 2553 2553 Size: 6865 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 4069 1700 Size: 2369 4496 SmaI CCC'GGG Cuts at: 1872 2549 2641 2802 4192 1872 Size: 677 92 161 1390 4545 Fragments arranged by size: 4545 1390 677 161 92 SmlI C'TyrA_G Cuts at: 820 1017 1943 2661 4157 4969 5870 6132 820 Size: 197 926 718 1496 812 901 262 1553 Fragments arranged by size: 1553 1496 926 901 812 718 262 197 SnaBI TAC'GTA Cuts at: 493 4656 493 Size: 4163 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6865 SphI G_CATG'C Cuts at: 1132 4223 1132 Size: 3091 3774 SspI AAT'ATT Cuts at: 5 52 4789 5 Size: 47 4737 2081 Fragments arranged by size: 4737 2081 47 StuI AGG'CCT Cuts at: 1517 4009 1517 Size: 2492 4373 StyI C'CwwG_G Cuts at: 513 1065 2004 4304 513 Size: 552 939 2300 3074 Fragments arranged by size: 3074 2300 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2471 3218 4892 4909 5062 5247 5692 Size: 1255 891 747 1674 17 153 185 445 Cuts at: 5692 325 Size: 1498 Fragments arranged by size: 1674 1498 1255 891 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2250 3812 5111 Size: 274 80 33 51 1443 273 1562 1299 Cuts at: 5111 6823 96 Size: 1712 138 Fragments arranged by size: 1712 1562 1443 1299 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6825 6857 6825 Size: 32 6833 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6865 XbaI T'CTAG_A Cuts at: 4201 4201 Size: 6865 XhoI C'TCGA_G Cuts at: 1943 2661 1943 Size: 718 6147 XmaI C'CCGG_G Cuts at: 1870 2547 2639 2800 4190 1870 Size: 677 92 161 1390 4545 Fragments arranged by size: 4545 1390 677 161 92 XmnI GAAnn'nnTTC Cuts at: 2028 4994 2028 Size: 2966 3899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4673 276 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI FseI FspAI NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI