(Circular) (Six-base) MAPSORT of: pFN21AB8707.seq Check: 4354 from: 1 to: 6322 pFN21AB8707 6322 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3526 3665 1937 Size: 1589 139 4594 Fragments arranged by size: 4594 1589 139 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3570 4385 5769 6037 1632 Size: 1938 815 1384 268 1917 Fragments arranged by size: 1938 1917 1384 815 268 AflII C'TTAA_G Cuts at: 820 1017 2554 820 Size: 197 1537 4588 Fragments arranged by size: 4588 1537 197 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 3476 4065 5637 3476 Size: 589 1572 4161 Fragments arranged by size: 4161 1572 589 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2273 2478 2669 2843 3208 3631 3857 4009 Size: 780 205 191 174 365 423 226 152 Cuts at: 4009 6118 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 780 423 365 226 205 191 174 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 3647 1870 Size: 73 1704 4545 Fragments arranged by size: 4545 1704 73 AvrII C'CTAG_G Cuts at: 2870 2870 Size: 6322 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3643 5098 618 Size: 325 217 627 1856 1455 1842 Fragments arranged by size: 1856 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3641 1391 Size: 2250 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 2997 928 Size: 971 186 912 4253 Fragments arranged by size: 4253 971 912 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2575 2609 4513 4547 1903 Size: 34 638 34 1904 34 3678 Fragments arranged by size: 3678 1904 638 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BfrBI ATG'CAT Cuts at: 2566 2566 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4933 136 Size: 107 122 71 631 605 3261 1525 Fragments arranged by size: 3261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 4383 5539 1790 Size: 2593 1156 2573 Fragments arranged by size: 2593 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4053 5011 442 Size: 416 53 83 399 24 2636 958 1753 Fragments arranged by size: 2636 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 4982 1239 Size: 1530 2213 2579 Fragments arranged by size: 2579 2213 1530 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4447 5312 5610 4447 Size: 865 298 5159 Fragments arranged by size: 5159 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3525 4985 882 Size: 617 2026 1460 2219 Fragments arranged by size: 2219 2026 1460 617 BsaAI yAC'GTr Cuts at: 493 1204 3380 4113 493 Size: 711 2176 733 2702 Fragments arranged by size: 2702 2176 733 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3325 4129 4511 5146 Size: 53 83 186 936 1792 804 382 635 Cuts at: 5146 275 Size: 1451 Fragments arranged by size: 1792 1451 936 804 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3194 4755 5153 5427 5574 5985 3194 Size: 1561 398 274 147 411 3531 Fragments arranged by size: 3531 1561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 1178 Size: 30 165 30 778 30 5289 Fragments arranged by size: 5289 778 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6322 BseYI C'CCAG_C Cuts at: 5525 6021 5525 Size: 496 5826 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3065 1163 Size: 573 1329 4420 Fragments arranged by size: 4420 1329 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3411 4383 4468 5539 3411 Size: 972 85 1071 4194 Fragments arranged by size: 4194 1071 972 85 BsiWI C'GTAC_G Cuts at: 2582 2582 Size: 6322 BsmI GAATG_Cn' Cuts at: 2639 3833 3926 2639 Size: 1194 93 5035 Fragments arranged by size: 5035 1194 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3316 3316 Size: 6322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3411 3641 4383 4468 5539 1391 Size: 399 1621 230 742 85 1071 2174 Fragments arranged by size: 2174 1621 1071 742 399 230 85 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 2074 2662 4211 5941 2074 Size: 588 1549 1730 2455 Fragments arranged by size: 2455 1730 1549 588 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3037 3677 844 Size: 2193 640 3489 Fragments arranged by size: 3489 2193 640 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4966 5153 1700 Size: 110 3156 187 2869 Fragments arranged by size: 3156 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstXI CCAn_nnnn'nTGG Cuts at: 2238 2238 Size: 6322 BstYI r'GATC_y Cuts at: 1972 3415 3652 4019 4404 4421 5873 5959 5971 Size: 1443 237 367 385 17 1452 86 12 Cuts at: 5971 6317 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1443 385 367 346 237 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2406 2406 Size: 6322 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4831 Fragments arranged by size: 4831 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 3838 4632 4652 3838 Size: 794 20 5508 Fragments arranged by size: 5508 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 2386 3626 2386 Size: 1240 5082 DraIII CAC_nnn'GTG Cuts at: 3383 3383 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287 Size: 54 1425 50 275 18 1939 889 1629 Cuts at: 6287 8 Size: 43 Fragments arranged by size: 1939 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2607 3154 3331 4252 1353 Size: 1181 73 547 177 921 3423 Fragments arranged by size: 3423 1181 921 547 177 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4909 5283 5429 387 Size: 1064 138 435 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2769 3570 4385 4982 5769 6037 1239 Size: 393 1137 801 815 597 787 268 1524 Fragments arranged by size: 1524 1137 815 801 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3179 3179 Size: 6322 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2102 2102 Size: 6322 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 5469 1678 Size: 3791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2535 2567 4963 4995 5037 Size: 32 135 32 963 32 2396 32 42 Cuts at: 5037 5069 1373 Size: 32 2626 Fragments arranged by size: 2626 2396 963 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2863 5436 5620 1305 Size: 1558 2573 184 2007 Fragments arranged by size: 2573 2007 1558 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4500 Fragments arranged by size: 4500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2236 3309 4280 4639 4798 518 Size: 648 1070 1073 971 359 159 2042 Fragments arranged by size: 2042 1073 1070 971 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3127 3738 4415 5563 5808 1606 Size: 1521 611 677 1148 245 2120 Fragments arranged by size: 2120 1521 1148 677 611 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4831 Fragments arranged by size: 4831 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NsiI A_TGCA'T Cuts at: 2568 2568 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2475 2566 3680 5225 1132 Size: 97 1246 91 1114 1545 2229 Fragments arranged by size: 2229 1545 1246 1114 97 91 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6322 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 3383 1108 Size: 2275 4047 PfoI T'CCnGG_A Cuts at: 2746 2746 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PmlI CAC'GTG Cuts at: 3380 3380 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3179 3179 Size: 6322 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PstI C_TGCA'G Cuts at: 830 3472 3674 830 Size: 2642 202 3478 Fragments arranged by size: 3478 2642 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 3127 3127 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 3502 4570 6282 3502 Size: 1068 1712 3542 Fragments arranged by size: 3542 1712 1068 SfcI C'TryA_G Cuts at: 826 1045 3110 3468 3670 4805 5486 5677 826 Size: 219 2065 358 202 1135 681 191 1471 Fragments arranged by size: 2065 1471 1135 681 358 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2554 4426 5327 5589 820 Size: 197 926 611 1872 901 262 1553 Fragments arranged by size: 1872 1553 926 901 611 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 2475 3680 1132 Size: 1343 1205 3774 Fragments arranged by size: 3774 1343 1205 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6322 StyI C'CwwG_G Cuts at: 513 1065 2004 2870 3761 513 Size: 552 939 866 891 3074 Fragments arranged by size: 3074 939 891 866 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2226 4349 4366 4519 4704 5149 325 Size: 1255 646 2123 17 153 185 445 1498 Fragments arranged by size: 2123 1498 1255 646 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3500 4568 6280 Size: 274 80 33 51 1443 1523 1068 1712 Cuts at: 6280 96 Size: 138 Fragments arranged by size: 1712 1523 1443 1068 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6282 6314 6282 Size: 32 6290 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3055 3658 3055 Size: 603 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2231 2238 2231 Size: 7 6315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 2337 3349 4451 2337 Size: 1012 1102 4208 Fragments arranged by size: 4208 1102 1012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI