(Circular) (Six-base) MAPSORT of: pFN21AB8702.seq Check: 2884 from: 1 to: 5818 pFN21AB8702 5818 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3016 3016 Size: 5818 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 3881 5265 5533 1632 Size: 491 1758 1384 268 1917 Fragments arranged by size: 1917 1758 1384 491 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 4717 5694 1203 Size: 3514 977 1327 Fragments arranged by size: 3514 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 3113 4547 3113 Size: 1434 4384 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2018 2326 2581 2632 2668 2887 3561 5133 2018 Size: 308 255 51 36 219 674 1572 2703 Fragments arranged by size: 2703 1572 674 308 255 219 51 36 ApaI G_GGCC'C Cuts at: 2256 2706 2256 Size: 450 5368 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 3127 3353 3505 5614 1493 Size: 1634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2054 2255 2402 2441 2699 2705 Size: 73 104 7 201 147 39 258 6 Cuts at: 2705 2948 3143 1870 Size: 243 195 4545 Fragments arranged by size: 4545 258 243 201 195 147 104 73 39 7 6 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2208 2381 2600 2689 Size: 325 217 627 252 169 173 219 89 Cuts at: 2689 2761 2783 3139 4594 618 Size: 72 22 356 1455 1842 Fragments arranged by size: 1842 1455 627 356 325 252 219 217 173 169 89 72 22 BanII G_rGCy'C Cuts at: 1391 2256 2706 2958 3137 1391 Size: 865 450 252 179 4072 Fragments arranged by size: 4072 865 450 252 179 BbeI G_GCGC'C Cuts at: 2043 2385 2604 2765 2787 2043 Size: 342 219 161 22 5074 Fragments arranged by size: 5074 342 219 161 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2605 928 Size: 971 706 4141 Fragments arranged by size: 4141 971 706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2031 2726 Size: 107 122 71 631 605 337 22 695 Cuts at: 2726 4429 136 Size: 1703 1525 Fragments arranged by size: 1703 1525 695 631 605 337 122 107 71 22 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 3054 3235 3054 Size: 181 5637 Bme1580I G_kGCm'C Cuts at: 1790 2256 2706 3879 5035 1790 Size: 466 450 1173 1156 2573 Fragments arranged by size: 2573 1173 1156 466 450 BmgBI CAC'GTC Cuts at: 1318 2797 1318 Size: 1479 4339 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3549 4507 442 Size: 416 53 83 399 24 2132 958 1753 Fragments arranged by size: 2132 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2584 2914 2584 Size: 330 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2909 2954 4478 1239 Size: 1262 408 45 1524 2579 Fragments arranged by size: 2579 1524 1262 408 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2382 2601 2714 Size: 53 83 186 936 507 342 219 113 Cuts at: 2714 2762 2784 3625 4007 4642 275 Size: 48 22 841 382 635 1451 Fragments arranged by size: 1451 936 841 635 507 382 342 219 186 113 83 53 48 22 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5593 Fragments arranged by size: 5593 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 2966 1186 Size: 1642 138 4038 Fragments arranged by size: 4038 1642 138 BseYI C'CCAG_C Cuts at: 2161 2407 2511 2526 2694 5021 5517 2161 Size: 246 104 15 168 2327 496 2462 Fragments arranged by size: 2462 2327 496 246 168 104 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2681 1163 Size: 573 645 300 4300 Fragments arranged by size: 4300 645 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2421 2481 2730 3268 4029 5057 Size: 275 186 420 60 249 538 761 1028 Cuts at: 5057 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 538 420 275 249 186 60 BsiHKAI G_wGCw'C Cuts at: 2646 2877 2958 3879 3964 5035 2646 Size: 231 81 921 85 1071 3429 Fragments arranged by size: 3429 1071 921 231 85 81 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2646 2706 2877 2958 3137 3879 Size: 399 466 390 60 171 81 179 742 Cuts at: 3879 3964 5035 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 399 390 179 171 85 81 60 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3016 3173 844 Size: 2172 157 3489 Fragments arranged by size: 3489 2172 157 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2082 2141 2416 2476 2489 2597 Size: 110 232 40 59 275 60 13 108 Cuts at: 2597 2686 2725 4462 4649 1700 Size: 89 39 1737 187 2869 Fragments arranged by size: 2869 1737 275 232 187 110 108 89 60 59 40 39 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 2176 2225 2315 2503 2585 1925 Size: 251 49 90 188 82 5158 Fragments arranged by size: 5158 251 188 90 82 49 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 2018 2668 794 Size: 1224 650 3944 Fragments arranged by size: 3944 1224 650 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2377 Size: 5818 BstYI r'GATC_y Cuts at: 1972 3148 3515 3900 3917 5369 5455 5467 5813 Size: 1176 367 385 17 1452 86 12 346 Cuts at: 5813 1972 Size: 1977 Fragments arranged by size: 1977 1452 1176 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3103 3103 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2248 2385 2920 513 Size: 552 1183 137 535 3411 Fragments arranged by size: 3411 1183 552 535 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 2666 3334 4128 4148 2666 Size: 668 794 20 4336 Fragments arranged by size: 4336 794 668 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DraIII CAC_nnn'GTG Cuts at: 2659 2659 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2801 4825 809 Size: 507 1485 2024 1802 Fragments arranged by size: 2024 1802 1485 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2418 2448 2478 Size: 54 1425 50 275 18 588 30 30 Cuts at: 2478 2538 2727 2938 3038 3265 4154 5783 8 Size: 60 189 211 100 227 889 1629 43 Fragments arranged by size: 1629 1425 889 588 275 227 211 189 100 60 54 50 43 30 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2418 2478 2727 3265 1537 Size: 275 606 60 249 538 4090 Fragments arranged by size: 4090 606 538 275 249 60 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2297 3748 1353 Size: 896 48 1451 3423 Fragments arranged by size: 3423 1451 896 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 4405 4779 4925 387 Size: 1064 138 561 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 561 374 146 138 EcoICRI GAG'CTC Cuts at: 2956 2956 Size: 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2501 2909 2954 3881 4478 5265 Size: 393 491 378 408 45 927 597 787 Cuts at: 5265 5533 1239 Size: 268 1524 Fragments arranged by size: 1524 927 787 597 491 408 393 378 268 45 EcoNI CCTnn'n_nnAGG Cuts at: 2276 2300 2276 Size: 24 5794 EcoO109I rG'GnC_Cy Cuts at: 2051 2051 Size: 5818 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2043 2243 2267 2326 2376 2385 2555 2604 Size: 365 200 24 59 50 9 170 49 Cuts at: 2604 2632 2765 2787 4965 1678 Size: 28 133 22 2178 2531 Fragments arranged by size: 2531 2178 365 200 170 133 59 50 49 28 24 22 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3099 3131 4459 4491 4533 Size: 32 135 32 1527 32 1328 32 42 Cuts at: 4533 4565 1373 Size: 32 2626 Fragments arranged by size: 2626 1527 1328 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3162 3408 669 Size: 988 281 1110 114 246 3079 Fragments arranged by size: 3079 1110 988 281 246 114 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KasI G'GCGC_C Cuts at: 2039 2381 2600 2761 2783 2039 Size: 342 219 161 22 5074 Fragments arranged by size: 5074 342 219 161 22 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4932 5116 1305 Size: 3627 184 2007 Fragments arranged by size: 3627 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3040 10 Size: 54 1425 343 1208 2788 Fragments arranged by size: 2788 1425 1208 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3776 4135 4294 518 Size: 648 1455 1155 359 159 2042 Fragments arranged by size: 2042 1455 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2161 2215 2356 2387 2521 2530 2578 Size: 409 146 54 141 31 134 9 48 Cuts at: 2578 2808 2937 3234 3911 5059 5304 1606 Size: 230 129 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 409 297 245 230 146 141 134 129 54 48 31 9 NaeI GCC'GGC Cuts at: 2044 2143 2418 2478 2688 2044 Size: 99 275 60 210 5174 Fragments arranged by size: 5174 275 210 99 60 NarI GG'CG_CC Cuts at: 2040 2382 2601 2762 2784 2040 Size: 342 219 161 22 5074 Fragments arranged by size: 5074 342 219 161 22 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5266 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 2042 2141 2416 2476 2686 2042 Size: 99 275 60 210 5174 Fragments arranged by size: 5174 275 210 99 60 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2626 3176 4721 1132 Size: 97 1397 550 1545 2229 Fragments arranged by size: 2229 1545 1397 550 97 PasI CC'CwG_GG Cuts at: 1348 2377 2779 1348 Size: 1029 402 4387 Fragments arranged by size: 4387 1029 402 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 2581 2887 2977 1108 Size: 1218 255 306 90 3949 Fragments arranged by size: 3949 1218 306 255 90 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2770 3887 3919 5400 5432 2738 Size: 32 1117 32 1481 32 3124 Fragments arranged by size: 3124 1481 1117 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2051 2051 Size: 5818 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 2252 2702 2252 Size: 450 5368 PspXI vC'TCGA_Gb Cuts at: 2402 2441 2402 Size: 39 5779 PstI C_TGCA'G Cuts at: 830 2023 2361 2550 3027 3170 830 Size: 1193 338 189 477 143 3478 Fragments arranged by size: 3478 1193 477 338 189 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2161 2215 2521 2530 2578 2161 Size: 54 306 9 48 5401 Fragments arranged by size: 5401 306 54 48 9 SacI G_AGCT'C Cuts at: 2958 2958 Size: 5818 SacII CC_GC'GG Cuts at: 2388 2388 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2051 2051 Size: 5818 SapI GCTCTTCn'nnn_ Cuts at: 2249 2249 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 2897 4066 5778 2897 Size: 1169 1712 2937 Fragments arranged by size: 2937 1712 1169 SfcI C'TryA_G Cuts at: 826 1045 2019 2357 2546 3023 3166 4301 4982 Size: 219 974 338 189 477 143 1135 681 Cuts at: 4982 5173 826 Size: 191 1471 Fragments arranged by size: 1471 1135 974 681 477 338 219 191 189 143 SfoI GGC'GCC Cuts at: 2041 2383 2602 2763 2785 2041 Size: 342 219 161 22 5074 Fragments arranged by size: 5074 342 219 161 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 2597 1700 Size: 897 4921 SmaI CCC'GGG Cuts at: 1872 2049 2257 2701 2707 3145 1872 Size: 177 208 444 6 438 4545 Fragments arranged by size: 4545 444 438 208 177 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 2441 3922 4823 5085 820 Size: 197 926 459 39 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 459 262 197 39 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 2626 3176 1132 Size: 1494 550 3774 Fragments arranged by size: 3774 1494 550 SrfI GCCC'GGGC Cuts at: 2701 2701 Size: 5818 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2025 1517 Size: 508 5310 StyI C'CwwG_G Cuts at: 513 1065 3257 513 Size: 552 2192 3074 Fragments arranged by size: 3074 2192 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 4064 5776 Size: 274 80 33 51 1443 918 1169 1712 Cuts at: 5776 96 Size: 138 Fragments arranged by size: 1712 1443 1169 918 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5778 5810 5778 Size: 32 5786 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 2402 2441 1943 Size: 459 39 5320 Fragments arranged by size: 5320 459 39 XmaI C'CCGG_G Cuts at: 1870 2047 2255 2699 2705 3143 1870 Size: 177 208 444 6 438 4545 Fragments arranged by size: 4545 444 438 208 177 6 XmnI GAAnn'nnTTC Cuts at: 2111 3947 2111 Size: 1836 3982 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SexAI SfiI SwaI XcmI