(Circular) (Six-base) MAPSORT of: pFN21AB8694.seq Check: 9507 from: 1 to: 6070 pFN21AB8694 6070 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2458 3413 1937 Size: 521 955 4594 Fragments arranged by size: 4594 955 521 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2633 2684 3237 4133 5517 5785 1632 Size: 1001 51 553 896 1384 268 1917 Fragments arranged by size: 1917 1384 1001 896 553 268 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 3208 4969 5946 1203 Size: 2005 1761 977 1327 Fragments arranged by size: 2005 1761 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 2213 2820 3813 5385 2213 Size: 607 993 1572 2898 Fragments arranged by size: 2898 1572 993 607 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2747 3379 3605 3757 5866 1493 Size: 1254 632 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2307 2592 3395 1870 Size: 73 72 292 285 803 4545 Fragments arranged by size: 4545 803 292 285 73 72 AvrII C'CTAG_G Cuts at: 2911 2911 Size: 6070 BamHI G'GATC_C Cuts at: 2915 3400 3767 2915 Size: 485 367 5218 Fragments arranged by size: 5218 485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2231 3391 4846 618 Size: 325 217 627 224 220 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 325 224 220 217 BanII G_rGCy'C Cuts at: 1391 2506 3389 1391 Size: 1115 883 4072 Fragments arranged by size: 4072 1115 883 BbeI G_GCGC'C Cuts at: 2235 2235 Size: 6070 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2749 3962 5172 2749 Size: 1213 1210 3647 Fragments arranged by size: 3647 1213 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4681 136 Size: 107 122 71 631 605 337 2672 1525 Fragments arranged by size: 2672 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2325 6065 2325 Size: 3740 2330 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 4131 5287 1790 Size: 2341 1156 2573 Fragments arranged by size: 2573 2341 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 3309 3360 4730 1239 Size: 1046 1024 51 1370 2579 Fragments arranged by size: 2579 1370 1046 1024 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2518 4195 5060 5358 2518 Size: 1677 865 298 3230 Fragments arranged by size: 3230 1677 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2261 4733 882 Size: 617 762 2472 2219 Fragments arranged by size: 2472 2219 762 617 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 3877 4259 4894 Size: 53 83 186 936 699 1645 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1645 1451 936 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3083 4503 4901 5175 5322 5733 3083 Size: 1420 398 274 147 411 3420 Fragments arranged by size: 3420 1420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5845 Fragments arranged by size: 5845 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 5178 BseYI C'CCAG_C Cuts at: 2046 3074 5273 5769 2046 Size: 1028 2199 496 2347 Fragments arranged by size: 2347 2199 1028 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 1163 Size: 573 651 4846 Fragments arranged by size: 4846 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2536 3520 4281 5309 1540 Size: 275 186 36 499 984 761 1028 2301 Fragments arranged by size: 2301 1028 984 761 499 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2506 4131 4216 5287 2506 Size: 1625 85 1071 3289 Fragments arranged by size: 3289 1625 1071 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2506 3389 4131 4216 5287 1391 Size: 399 716 883 742 85 1071 2174 Fragments arranged by size: 2174 1071 883 742 716 399 85 BspEI T'CCGG_A Cuts at: 3083 5733 3083 Size: 2650 3420 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3425 844 Size: 2581 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2325 2574 2915 3400 3767 4152 4169 5621 Size: 353 249 341 485 367 385 17 1452 Cuts at: 5621 5707 5719 6065 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 485 385 367 353 346 341 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2884 513 Size: 552 939 880 3699 Fragments arranged by size: 3699 939 880 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 2258 2868 3586 4380 4400 2258 Size: 610 718 794 20 3928 Fragments arranged by size: 3928 794 718 610 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 2083 3374 2083 Size: 1291 4779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2041 2895 5077 809 Size: 507 725 854 2182 1802 Fragments arranged by size: 2182 1802 854 725 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3517 4406 6035 Size: 54 1425 50 275 18 1687 889 1629 Cuts at: 6035 8 Size: 43 Fragments arranged by size: 1687 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2618 3131 4000 1353 Size: 702 563 513 869 3423 Fragments arranged by size: 3423 869 702 563 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2504 2504 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2633 2684 3237 3309 3360 4133 Size: 393 653 348 51 553 72 51 773 Cuts at: 4133 4730 5517 5785 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 773 653 597 553 393 348 268 72 51 51 EcoO109I rG'GnC_Cy Cuts at: 2352 2352 Size: 6070 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 6070 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 2235 2911 3082 5217 1678 Size: 557 676 171 2135 2531 Fragments arranged by size: 2531 2135 676 557 171 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 4711 4743 4785 Size: 32 135 32 815 32 2292 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 2292 815 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2459 2569 3013 3414 3660 669 Size: 988 281 521 110 444 401 246 3079 Fragments arranged by size: 3079 988 521 444 401 281 246 110 HindIII A'AGCT_T Cuts at: 748 2115 748 Size: 1367 4703 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KasI G'GCGC_C Cuts at: 2231 2231 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 3208 5946 3208 Size: 2738 3332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2057 3197 5184 5368 1305 Size: 752 1140 1987 184 2007 Fragments arranged by size: 2007 1987 1140 752 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4248 Fragments arranged by size: 4248 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3000 4028 4387 4546 518 Size: 648 1834 1028 359 159 2042 Fragments arranged by size: 2042 1834 1028 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3486 4163 5311 5556 1606 Size: 1880 677 1148 245 2120 Fragments arranged by size: 2120 1880 1148 677 245 NarI GG'CG_CC Cuts at: 2232 2232 Size: 6070 NcoI C'CATG_G Cuts at: 513 1065 2004 2884 513 Size: 552 939 880 3699 Fragments arranged by size: 3699 939 880 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NruI TCG'CGA Cuts at: 3277 3277 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 3428 4973 1132 Size: 97 2199 1545 2229 Fragments arranged by size: 2229 2199 1545 97 PasI CC'CwG_GG Cuts at: 1348 2347 1348 Size: 999 5071 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2352 2352 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PstI C_TGCA'G Cuts at: 830 2816 3422 830 Size: 1986 606 3478 Fragments arranged by size: 3478 1986 606 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 SacI G_AGCT'C Cuts at: 2506 2506 Size: 6070 SalI G'TCGA_C Cuts at: 1936 2457 3412 1936 Size: 521 955 4594 Fragments arranged by size: 4594 955 521 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SexAI A'CCwGG_T Cuts at: 2763 3163 2763 Size: 400 5670 SfcI C'TryA_G Cuts at: 826 1045 2812 3418 4553 5234 5425 826 Size: 219 1767 606 1135 681 191 1471 Fragments arranged by size: 1767 1471 1135 681 606 219 191 SfoI GGC'GCC Cuts at: 2233 2233 Size: 6070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2497 2592 4174 5075 5337 820 Size: 197 926 554 95 1582 901 262 1553 Fragments arranged by size: 1582 1553 926 901 554 262 197 95 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 2726 3994 5 Size: 47 2674 1268 2081 Fragments arranged by size: 2674 2081 1268 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2004 2884 2911 2937 3036 3509 513 Size: 552 939 880 27 26 99 473 3074 Fragments arranged by size: 3074 939 880 552 473 99 27 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 2081 2550 4097 4114 4267 4452 Size: 1255 471 30 469 1547 17 153 185 Cuts at: 4452 4897 325 Size: 445 1498 Fragments arranged by size: 1547 1498 1255 471 469 445 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4316 6028 96 Size: 274 80 33 51 1443 2339 1712 138 Fragments arranged by size: 2339 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3352 3384 6030 6062 3352 Size: 32 2646 32 3360 Fragments arranged by size: 3360 2646 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 2592 1943 Size: 649 5421 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 2166 2395 2489 2606 4199 2166 Size: 229 94 117 1593 4037 Fragments arranged by size: 4037 1593 229 117 94 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI